The Effect of Carprofen Treatment on Conception Rate After Insemination in Cows with Increased Days in Milk

Abstract

In this study the effect of carprofen treatment on conception rate after insemination in Holstein cows that did not conceive for a long time after parturition was evaluated. In the study, 200 Holstein cows with days in milk >120 were used. A progesterone + ovsynch-based estrus synchronization protocol was applied to the cows included in the study. For this purpose, the progesterone device was placed intravaginally together with GnRH injection. Seven days later, the progesterone source was removed from the vagina and PGF2α injection was administered. GnRH injection was administered 48 hours following PGF2α injection and fixed-time insemination was performed 12-16 hours later. After insemination, animals were randomly divided into 2 groups. Subcutaneous carprofen was applied to the carprofen group (n = 100) on the 14th day after insemination and physiological saline was applied to the control group (n = 100) on the same day. Three cows from the carprofen group and 5 cows from the control group were excluded from the study for various reasons. The pregnancy rate was 42.26% (41/97) in the carprofen group and 25.26% (24/95) in the control group (p <0.05). However, the rate of conception was found to be the lowest (10.52%) in cows with high milk yield (>30 kg) in the control group. In addition, it was found that carprofen administration to cows with high milk yield increased the rate of conception. However, it was determined that the days in milk had no effect on pregnancy rate. As a result, it was concluded that the administration of carprofen on the 14th day after insemination to cows who could not conceive for a long time after parturition may be effective in increasing the rate of conception.

Keywords

• Carprofen
• cow
• days in milk
• milk yield
• pregnancy rate

Sağımda Geçen Gün Süresi Artmış Olan İneklerde Tohumlama Sonrası Karprofen Uygulamasının Gebe Kalma Oranı Üzerine Etkisi

Öz

Bu çalışmada doğumdan sonra uzun süre gebeliğin sağlanamadığı Holstein ırkı ineklerde, tohumlama sonrası karprofen uygulamasının gebe kalma oranı üzerine etkisi değerlendirilmiştir. Çalışmada sağımda geçen gün süresi ˃120 gün olan 200 Holstein ırkı inek kullanıldı. Çalışmaya dahil edilen ineklere progesteron+ovsynch esaslı östrüs senkronizasyon protokolü uygulandı. Bu amaçla GnRH enjeksiyonu ile birlikte progesteron kaynağı intravaginal olarak yerleştirildi. Yedi gün sonra progesteron kaynağı vaginadan uzaklaştırıldı ve PGF2α enjeksiyonu yapıldı. PGF2α enjeksiyonundan 48 saat sonra GnRH enjeksiyonu uygulandı ve 12-16 saat sonra ineklere sabit zamanlı tohumlama yapıldı. Tohumlama sonrası hayvanlar rastgele olarak 2 gruba ayrıldı. Karprofen grubuna (n=100) tohumlama sonrası 14. gün deri altı karprofen ve kontrol grubuna (n=100) ise aynı gün fizyolojik tuzlu su uygulandı. Karprofen grubundan 3 ve kontrol grubundan ise 5 inek çeşitli nedenlerle çalışmadan çıkarıldı. Gebe kalma oranı; karprofen grubunda %42,26 (41/97), kontrol grubunda ise %25,26 (24/95) olarak belirlendi (p<0,05). Bununla birlikte kontrol grubunda yüksek süt verimli (≥30 kg) ineklerde gebe kalma oranları en düşük (%10,52) bulundu. Ayrıca yüksek süt verimli ineklere karprofen uygulamasının gebe kalma oranını artırdığı tespit edildi. Ancak sağımda geçen gün süresinin gebelik oranı üzerine etkisinin olmadığı belirlendi. Sonuç olarak doğumdan sonra uzun bir süre gebe kalamamış ineklere, tohumlama sonrası 14. günde karprofen uygulamasının gebe kalma oranını artırmada etkili olabileceği kanısına varıldı.

Anahtar Kelimeler

• Gebelik oranı
• inek
• karprofen
• sağımda geçen gün süresi
• süt verimi

References

1. Alkan H, Erdem H. Repeat breeder ineklerde tohumlama sonrası yapılan hormonal desteğe, laktasyon sayısı, süt verimi ve sağımda geçen gün süresinin etkisi. Eurasian J Vet Sci. 2020; 36(4): 298-305.
2. Alkan H, Erdem H. Reproductive use of nonsteroidal antiinflammatory drugs in cows. Ataturk Univ Vet Bil Derg. 2018; 13(1): 112-120.
3. Amiridis GS, Tsiligianni T, Dovolou E, Rekkas C, Vouzaras D, Menegatos I. Combined administration of gonadotropin-releasing hormone, progesterone, and meloxicam is an effective treatment for the repeat-breeder cow. Theriogenology. 2009; 72(4): 542-548.
4. Bartlett PC, Kirk JH, Mather EC. Repeated insemination in Michigan Holstein-Friesian cattle: incidence, descriptive epidemiology and estimated economic impact. Theriogenelogy. 1986; 26(3): 309-322.
5. Bedere N, Cutullic E, Delaby L, Garcia-Launay F, Disenhaus C. Meta-analysis of the relationships between reproduction, milk yield and body condition score in dairy cows. Livest Sci. 2018; 210: 73-84.
6. Berry DP, Friggens NC, Lucy M, Roche JR. Milk production and fertility in cattle. Annu Rev Anim Biosci. 2016; 4: 269-290. Binelli M, Thatcher WW, Mattos R, Baruselli PS. Antiluteolytic strategies to improve fertility in cattle. Theriogenology, 2001; 56(9): 1451-1463.
7. Buckley F, O'sullivan K, Mee JF, Evans RD, Dillon P. Relationships among milk yield, body condition, cow weight, and reproduction in spring-calved Holstein-Friesians. J Dairy Sci. 2003; 86(7): 2308-2319.
8. De Vries A. Determinants of the cost of days open in dairy cattle. In proceedings of the 11th international symposium on veterinary epidemiology and economics. 2006; pp. 19-20.

9. Diskin MG, Parr MH, Morris DG. Embryo death in cattle: an update. Reprod Fertil Dev. 2012; 24(1): 244-251.
10. Diskin MG, Waters SM, Parr MH, Kenny DA. Pregnancy losses in cattle: potential for improvement. Reprod Fertil Dev. 2016; 28(2): 83-93.
11. Dochi O, Takahashi K, Hirai T, Hayakawa H, Tanisawa M, Yamamoto Y, Koyama H. The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle. Theriogenology. 2008; 69 (1):124-128.
12. Doğruer G, Sarıbay MK, Karaca F. Repeat breeder sorunlu düvelerde fluniksin meglumin uygulamalarının gebelik oranı üzerine etkisi. Fırat Univ Saglık Bilim Derg. 2007; 21(6): 263-268.
13. Dursun Ş. Laktasyonda olmayan İsviçre Esmeri İnek ve düvelerde ketoprofen ve flunixin meglumin uygulamasının gebe kalma oranı üzerine etki. Doktora Tezi, Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü, Konya, 2011.
14. Emre B, Zonturlu AK, Korkmaz Ö. Sütçü ineklerde ovsynch protokolünü takiben uygulanan fluniksin meglumin’in gebelik oranı üzerine etkisi. Harran Üniv Vet Fak Derg. 2012; 1(2): 88-91.
15. Erdem H, Güzeloğlu A. Effect of meloxicam treatment during early pregnancy in holstein heifers. Reprod Dom Anim. 2010; 45: 625–628.
16. Ferguson JD, Skidmore A. Reproductive performance in a select sample of dairy herds. J Dairy Sci. 2013; 96(2): 1269-1289.
17. Fischer‐Tenhagen C, Thiele G, Heuwieser W, Tenhagen BA. Efficacy of a treatment with hCG 4 days after AI to reduce pregnancy losses in lactating dairy cows after synchronized ovulation. Reprod Domest Anim. 2010; 45(3): 468-472.
18. Gabor G, Tóth F., Ózsvári L, Abonyi‐Tóth Z, Sasser RG. Factors influencing pregnancy rate and late embryonic loss in dairy cattle. Reprod Domest Anim. 2008; 43(1): 53-58.
19. Güzeloğlu A, Erdem H, Saribay MK, Thatcher WW, Tekeli T. Effect of the administration of flunixin meglumine on pregnancy rates in Holstein heifers. Vet Rec. 2007; 160: 404-406.
20. Hagiya K, Terawaki Y, Yamazaki T, Nagamine Y, Itoh F, Yamaguchi S, Abe H, Gotoh Y, Kawahara T, Masuda Y, Suzuki M. Relationships between conception rate in Holstein heifers and cows and milk yield at various stages of lactation. Animal. 2013; 7(9): 1423.
21. Inskeep EK, Dailey RA. Embryonic death in cattle. Vet Clin Food Anim Prac. 2005; 21(2): 437-461.
22. LeBlanc SJ. Is a high level of milk production compatible with good reproductive performance in dairy cows?. Anim Front. 2013; 3(4): 84-91.
23. Lonergan P, Forde N. Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle. Animal. 2014; 8(s1): 64-69.
24. Lucacin E, Pinto-Neto A, Mota M, Acco A, Souza M, Alberton J, Silva A. Effects of flunixin meglumine on reproductive parameters in beef cattle. Anim Reprod. 2018; 7(2): 75-79.
25. Mann GE, Lamming GE. The influence of progesterone during early pregnancy in cattle. Reprod Dom. 1999; 34: 269-274.
26. Mellado M, Zuñiga A, Veliz FG, de Santiago A, Garcia JE, Mellado J. Factors influencing pregnancy per artificial insemination in repeat-breeder cows induced to ovulate with a CIDR-based protocol. Anim Reprod Sci. 2012; 134(3-4): 105-111.
27. Mishra S, Kumari K, Dubey A. Body condition scoring of dairy cattle: A review. RRJVS, 2016; 2, 58-65.
28. Morris D, Diskin M. Effect of progesterone on embryo survival. Animal. 2008; 2(8):1112-1119.
29. Mortimer RG, Farin PW, Stevens RD. Reproductive examination of the non- pregnant cow. In: Current Therapy in Large Animal Theriogenology. Ed; Youngquist RS, W.B Saunders Co., Philadelphia, USA. 1997; pp.268-275
30. Nebel RL, McGilliard ML. Interactions of high milk yield and reproductive performance in dairy cows. J Dairy Sci. 1993; 76(10): 3257-3268.
31. Odensvik K, Gustafsson H, Kindahl H. The effect on luteolysis by intensive oral administration of flunixin granules in heifers. Anim Reprod Sci. 1998; 50(1-2): 35-44.
32. Perez-Marin CC, Moreno LM, Calero GV. Clinical approach to the repeat breeder cow syndrome. In: A bird's-eye view of veterinary medicine, Ed; Perez-Marin CC, 1st Ed, Intech, Rijeka, Croatia. 2012; pp. 337-362.
33. Santos JEP, Thatcher WW, Chebel RC, Cerri RLA, Galvao KN. The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim Reprod Sci. 2004; 82: 513-535.
34. Shams-Esfanabadi N, Shirazi A. Effects of supplementation of repeat-breeder dairy cows with CIDR from 5-19 post-insemination on pregnancy rate. Pakistan J Biol Sci, 2006; 9(11): 2173-2176.
35. Shams-Esfandabadi N, Shirazi A, Mirshokrai P, Bonyadian M. Influence of hCG administration after AI on conception rates and serum progesterone concentration in cattle. PJBS, 2007; 10(16): 2709.
36. Spencer TE, Forde N, Dorniak P, Hansen TR, Romero JJ, Lonergan P. Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants. Reproduction. 2013; 146(4): 377-387.
37. Thatcher WW, Binelli M, Burke J, Staples CR, Ambrose JD, Coelho S. Antiluteolytic signals between the conceptus and endometrium. Theriogenology, 1997; 47(1): 131-140.
38. Thatcher WW, Guzeloglu A, Bilby TR. Early embryonic mortality in modern dairy cows: causes, consequences and remedies. Vlaams Dıergen Tıjds. 2006; 75: 106-113.
39. Thatcher WW, Staples CR, Danet-Desnoyers G, Oldick B, Schmitt EP. Embryo health and mortality in sheep and cattle. J Anim Sci Technol. 1994; 72: 16-30.
40. Vasconcelos JLM, Demétrio DGB, Santos RM, Chiari JR, Rodrigues CA, Sa Filho OG. Factors potentially affecting fertility of lactating dairy cow recipients. Theriogenology, 2006; 65(1): 192-200.
41. Villarroel A, Martino A, BonDurant RH, Dèletang F, Sischo WM. Effect of post-insemination supplementation with PRID on pregnancy in repeat-breeder Holstein cows. Theriogenology, 2004; 61(7-8): 1513-1520.
42. von Krueger X, Heuwieser W. Effect of flunixin meglumine and carprofen on pregnancy rates in dairy cattle. J Dairy Sci. 2010; 93: 5140–5146.
43. Walsh SW, Williams EJ, Evans ACO. A review of the causes of poor fertility in high milk producing dairy cows. Anim Reprod Sci. 2011; 123(3-4): 127-138.
44. Wathes C, Taylor V. Fertility in High Yielding Dairy Cows. Holstein J. 2002; 1: 108-118.
45. Watson AJ. Oocyte cytoplasmic maturation: a key mediator of oocyte and embryo developmental competence. J Anim Sci. 2007; 85(suppl_13): E1-E3.
46. Weems CW, Weems YS, Randel RD. Prostaglandins and reproduction in female farm animals. Vet J, 2006; 171(2): 206-228.
47. Wiltbank MC, Baez GM, Garcia-Guerra A, Toledo MZ, Monteiro PL, Melo LF, Ochoa JC, Santos JEP, Sartori R. Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows. Theriogenology. 2016; 86(1): 239-253.
48. Young CD. Reproductive efficiency following administration of an inhibitor of prostaglandin F2α during early embryonic development in dairy cattle. Master thesis, The University of Tennessee, Knoxville, 2004.