Comparison of two Hematocrite Measurement Methods in Diarrheic Neonatal Calves

Year 2018, Volume: 11 Issue: 1, 24 - 29, 01.03.2018

Hasan Erdoğan , Mehmet Gültekin Serdar Paşa Kerem Ural

Abstract

In
this study, it was aimed to compare two different hematocrit measurement techniques
which are commonly used in veterinary medicine by cell count, and amperometric
method. A total of 84 neonatal
diarrheic calves from both sexes with different etiologies were included in the
study. For hematocrit measurements, blood samples (4 ml) were taken to blood
gas injectors and tubes containing lithium heparin from calves. The Abacus
Junior Vet 5 whole blood cell counter and Irma True Point blood gas analyzer
were used for hematocrit measurements. According
to the results of linear regression analysis and Pearson correlation, it was
determined that the data obtained from the hematocrit measurements had positive
correlations between r = 0.90 (p <0.000) between the two measurement
techniques, but statistically significant differences were found between the
mean values of the two test techniques. In Passing-bablok analysis y= 0.88x+1.81 equation was found. According to the results, both measurements can be utilized in
assessing hematocrit measurement, but in case of the difference between the two
measuring methods, it was concluded that suspected cases should be made based
on the conventional measurement microhematocrit centrifugation.

Keywords

Calf, Diarrhoea, Hematocrit, Measurement technique, Passing-bablok

Neonatal İshalli Buzağılarda İki Farklı Hematokrit Ölçüm Tekniğinin Karşılaştırılması

Abstract

Bu çalışmada veteriner hekimlikte sıklıkla kullanılan
hücre sayımı temel prensipli ve amperometrik yöntem ile ölçüm yapan iki farklı
hematokrit ölçüm tekniğinin karşılaştırılması amaçlandı. Farklı etiyolojileri
bulunan her iki cinsiyetten toplam 84 neonatal ishalli buzağı çalışmaya dahil
edildi. Hematokrit ölçümleri için buzağılardan lityum heparin içeren kan gazı
enjektörlerine ve tüplere kan örnekleri, toplamda 4 ml olacak şekilde alındı.
Hematokrit ölçümleri için Abacus Junior Vet 5 kan sayım cihazı ve Irma
Truepoint kan gazı analiz cihazından yararlanıldı. Linear regresyon analizi ve pearson korelasyonu sonuçlarına göre
hematokrit ölçümlerinden elde edilen verilerin her iki ölçüm tekniği arasında
r=0.90 (p<0.000) düzeyinde pozitif yönde korelasyona sahip olduğu, ancak iki
test tekniğinin ortalama değerleri arasında istatistiksel yönden farklılıkların
bulunduğu belirlendi. Passing-bablok regresyon analizinde ise y= 0.88x+1.81 denklemi elde edildi. Çalışma sonuçlarına göre, klinikte
yoğun şekilde yapılan hematokrit ölçümlerinin değerlendirilmesinde her iki
ölçüm tekniğinden yararlanılabileceği ancak iki ölçüm metodu arasında
farklılıkların bulunmasına bağlı olarak hasta hayvanlarda şüpheye düşüldüğü
durumlarda klasik santrifüj yöntemine dayalı mikrohematokrit ölçümlerinin
yapılması gerektiği sonucuna varıldı.

Keywords

Buzağı, Hematokrit, İshal, Ölçüm tekniği, Passing-bablok

References

• Anonim.http://www.beckman.com/products/instrument/partchar/technology/coulterprinciple.asp. Erişim tarihi: 30.10.2017.
• Bamberg R, Gwyn T, Miller J, Thompson M, Transou P. The effects of over-anticoagulated blood on hematocrit values by the microcentrifuge method. Clin Lab Sci, 2008; 21(3), 146. Brian SB, Karen LH. Is the Packed Cell Volume (PCV) Reliable? Lab Hematol. 2001; 7: 191-196.
• Bull BS, Fujimoto K, Houwen B, Klee G, van Hove L, Van Assendelft OW, Lewis SM. International Council for Standardization in Haematology (ICSH) Recommendations for “Surrogate Reference” Method for the Packed Cell Volume. Lab Hematol. 2003; 9: 1-9.
• Bull BS, Koepke JA, Simson E. Procedure for Determining Packed Cell Volume by the Hematocrit Method. 3th Ed., . NCCLS publication H7-A3. Wayne, Pennsylvania, NCCLS, 2000.
• CLSI. Procedure for Determining Packed Cell Volume by the Microhematocrit Method; Approved Standard. 3rd Ed., Wayne, Pennsylvania, USA, 2000.
• Constable PD, Walker PG, Morın DE, Foreman JH. Clinical and Laboratory Assessment of Hydration Status of Neonatal Calves with Diarrhea. J Am Vet Med Assoc. 1998; 212: 991-996.
• Dubin S, Piszczek J, Beard R, Schmukler R. Effects of anticoagulants on packed cell volume measurement. Lab Anim Sci. 1976; 26: 586–591.
• El-sheikh AKR, Hayam M, Morsy S, Tarek H, Abbas A, Wafaa M. Clinical and laboratory examinations of diarrhea and dehydration in newborn friesian calves with special reference to therapy with hypertonic and isotonic solution. Life Sci J 2012; 9(4)
• Gebretsadkan TK, Ambachew G, Birhaneselassie H. The Comparison Between Microhematocrit and Automated Methods for Hematocrit Determination. Int J Blood Res Disord. 2015; 2(1): 1-3.
• Hartmann H, Reder S. Effect of Dehydration on Functional Indicators of Fluid Metabolism in Calves and the Efficacy of Rehydration with Crystalline or Colloidal Saline Infusions. Tierarztl Prax. 1995; 23: 342–350.
• Kathleen K. The Clinical Laboratory Improvement Act (CLIA) and the Physician’s Of ce Laboratory. Continuing Medical Education. 2007.
• Kronenberg F, Trenkwalder E, Kronenberg MF, König P, Utermann G, Dieplinger H. Influence of Hematocrit on the Measurement of Lipoproteins Demonstrated by the Example of Lipoprotein. Kidney Int. 1998; 54(4): 1385-1389.
• Linné JJ, Ringsrud KM. Principles and Practice of Clinical Hematology, In: Clinical Laboratory Science, the Basics and Routine Techniques, Ed; Turgeon ML, 4th Ed., St. Louis, Missouri, Mosby, 1999; pp. 279-295.
• McMahon DJ, Carpenter RL. A Comparison of Conductivity-based Hematocrit Determinations with convenTional Laboratory Methods in Autologous Blood Transfusions. Anesth Analg.1990; 71(5): 541-544.
• Mock T, Morrison D, Yatscoff R. Evaluation of the i-STAT™ System: A Portable Chemistry Analyzer for the Measurement of Sodium, Potassium, Chloride, Urea, Glucose, and Hematocrit. Clin Biochem. 1995; 28(2): 187-192.
• Peiró JR, Borges AS, Gonçalves RC, Mendes L. Evaluation of a portable clinical analyzer for the determination of blood gas partial pressures, electrolyte concentrations, and hematocrit in venous blood samples collected from cattle, horses, and sheep. Am J Vet Res. 2010; 71(5): 515-521.
• Pennock C, Jones K. Effect of ethylene-diamine- tetra-acetic (dipotassium salt) and heparin on the estimation of packed cell volume. J Clin Pathol. 1966; 19: 196–199.
• Prihirunkit K, Lekcharoensuk C, Pisetpaisan K. Comparison between manual and automated methods for determination of canine and feline hematocrit and hemoglobin concentration. Kasetsart Journal (Natural Science). 2008; 42(4): 655-659.
• Rao LV, Jakubiak F, Sidwell JS, Winkelman JW, Snyder ML. Accuracy evaluation of a new glucometer with automated hematocrit measurement and correction. Clini chim acta. 2005; 356(1): 178-183.
• Slanina L. Stoffwechselüberwachung in Kaelbernbestaende, In: Innere Krankheiten der Haustiere, Ed; Rossow N, Horvath Z, Bd ll: Funktionelle Störungen, G. Fischer, 1998; pp. 536-544.
• Smith GW, Berchtold J. Fluid Therapy in Calves. Vet Clin North Am Food Anim Pract. 2014;30(2):409-427
• Stott RAW, Hortin GL, Wilhite TR, Miller SB, Smith CH, Landt M. Analytical Artifacts in Hematocrit Measurements by Whole-blood Chemistry Analyzers. Clin Chem. 1995; 41(2): 306-311.
• Şen İ, Constable PD. General overview to treatment of strong ion (metabolic) acidosis in neonatal calves with diarrhea. Eurasian J Vet Sci, 2013; 29(3): 114-120.