Comparison of two Hematocrite Measurement Methods in Diarrheic Neonatal Calves

Yıl 2018, Cilt: 11 Sayı: 1, 24 - 29, 01.03.2018

Hasan Erdoğan Mehmet Gültekin Serdar Paşa Kerem Ural

Öz

In
this study, it was aimed to compare two different hematocrit measurement techniques
which are commonly used in veterinary medicine by cell count, and amperometric
method. A total of 84 neonatal
diarrheic calves from both sexes with different etiologies were included in the
study. For hematocrit measurements, blood samples (4 ml) were taken to blood
gas injectors and tubes containing lithium heparin from calves. The Abacus
Junior Vet 5 whole blood cell counter and Irma True Point blood gas analyzer
were used for hematocrit measurements. According
to the results of linear regression analysis and Pearson correlation, it was
determined that the data obtained from the hematocrit measurements had positive
correlations between r = 0.90 (p <0.000) between the two measurement
techniques, but statistically significant differences were found between the
mean values of the two test techniques. In Passing-bablok analysis y= 0.88x+1.81 equation was found. According to the results, both measurements can be utilized in
assessing hematocrit measurement, but in case of the difference between the two
measuring methods, it was concluded that suspected cases should be made based
on the conventional measurement microhematocrit centrifugation.

Anahtar Kelimeler

Calf, Diarrhoea, Hematocrit, Measurement technique, Passing-bablok

Neonatal İshalli Buzağılarda İki Farklı Hematokrit Ölçüm Tekniğinin Karşılaştırılması

Öz

Bu çalışmada veteriner hekimlikte sıklıkla kullanılan
hücre sayımı temel prensipli ve amperometrik yöntem ile ölçüm yapan iki farklı
hematokrit ölçüm tekniğinin karşılaştırılması amaçlandı. Farklı etiyolojileri
bulunan her iki cinsiyetten toplam 84 neonatal ishalli buzağı çalışmaya dahil
edildi. Hematokrit ölçümleri için buzağılardan lityum heparin içeren kan gazı
enjektörlerine ve tüplere kan örnekleri, toplamda 4 ml olacak şekilde alındı.
Hematokrit ölçümleri için Abacus Junior Vet 5 kan sayım cihazı ve Irma
Truepoint kan gazı analiz cihazından yararlanıldı. Linear regresyon analizi ve pearson korelasyonu sonuçlarına göre
hematokrit ölçümlerinden elde edilen verilerin her iki ölçüm tekniği arasında
r=0.90 (p<0.000) düzeyinde pozitif yönde korelasyona sahip olduğu, ancak iki
test tekniğinin ortalama değerleri arasında istatistiksel yönden farklılıkların
bulunduğu belirlendi. Passing-bablok regresyon analizinde ise y= 0.88x+1.81 denklemi elde edildi. Çalışma sonuçlarına göre, klinikte
yoğun şekilde yapılan hematokrit ölçümlerinin değerlendirilmesinde her iki
ölçüm tekniğinden yararlanılabileceği ancak iki ölçüm metodu arasında
farklılıkların bulunmasına bağlı olarak hasta hayvanlarda şüpheye düşüldüğü
durumlarda klasik santrifüj yöntemine dayalı mikrohematokrit ölçümlerinin
yapılması gerektiği sonucuna varıldı.

Anahtar Kelimeler

Buzağı, Hematokrit, İshal, Ölçüm tekniği, Passing-bablok

Kaynakça

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