Estimation of Volume of Ox Brain and Gray and White Matter with Cavalier’s Principle

Year 2018, Volume: 11 Issue: 1, 30 - 34, 01.03.2018

Durmuş Bolat

Abstract

We
estimated the volume values, ratios, and the relationship between brain weight and
volumetric values ​​of gray and white matter of the ox brain. Six fresh brains
of Holstein-Friesian strain healthy ox (17-20 months old) were used. Fresh
weights of the brains were measured using digital weighing. Brains were fixed
by immersion method in 10% formalin solution for 3 weeks before measurements of
gray and white matter volumes using Cavalieri’s principle. The brains were
serially cut using a salami slicer machine (Elta GmBH, Roedermark, Germany)
with a mean thickness of 8.25 mm from rostral to caudal end and averages of 11
sections were obtained from the brains. The brain volume was 348.00 ± 50.48 cm³,
175.70 ± 11.11 cm³ in gray matter volume and 172.30 ± 42.90 cm³ in white matter
volume. Volume ratios of gray and white matter were 50.98% and 49.02%
respectively. The ratio of brain weight to brain volume was 1. The ratio of
gray and white matter in the brain of the ox is similar to that of the gray and
white matter in the human brain, but that the sheep and horse are different.

 

Keywords

point counting method, brain volume, weight volume ratio, morphometry, stereology

Sığırlarda Beyin, Ak Madde ve Gri Maddenin Cavalieri Prensibi ile Hacim Hesaplamaları

Abstract

Bu
çalışmada sığır beynindeki gri ve beyaz maddenin hacim değerlerini, oranları ve
beyin ağırlığı ile volumetrik değerleri arasındaki ilişkiyi hesapladık.
Holstein-Friesian ırkı sağlıklı sığıra ait (17-20 aylık) altı taze beyin
kullanıldı. Beynin taze ağırlığı dijital tartım kullanılarak ölçüldü. Cavalieri
ilkesini kullanarak gri ve beyaz madde hacimlerinin ölçümünden önce beyinler
daldırma yöntemi ile % 10 formalin solüsyonunda 3 hafta tespit edildi. Beyinler
rostral’den kaudal’e doğru salam dilimleme makinesi (Elta GmBH, Roedermark,
Almanya) ile ortalama 8.25 mm kalınlıkta seri olarak kesildi ve beyinlerden
ortalama 11  kesit  elde edildi. Beyin hacmi 348.00 ± 50.48 cm³,
gri madde hacmi 175.70 ± 11.11 cm³ ve beyaz madde hacmide 172.30 ± 42.90 cm³
idi. Gri ve beyaz maddenin hacim oranları sırasıyla % 50.98 ve % 49.02' dir.
Beyin ağırlığının beyin hacmine oranı 1 idi. Sığır beynindeki gri ve beyaz
maddenin oranı insan beynindeki gri ve beyaz cevherinkine benzer, ancak
koyunlardan ve atlardan farklıdır.

Keywords

noktalı alan metodu, beyin hacmi, ağırlık hacim oranı, morfometri, stereoloji

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