Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells

Year 2019, Volume: 12 Issue: 3, 264 - 267, 30.09.2019

Hidayet Tutun

https://doi.org/10.30607/kvj.576695

Cited By: 1

Abstract

Melamine is an organic chemical used primarily as a
plastics stabilizer and fire retardant. Also, it was used to fraudulently
elevate the protein content of animal feed and dairy products, which led to
infant kidney diseases and pet deaths. In this study, melamine was studied for
its effects on growth in the human colon adenocarcinoma cells (Caco-2).
Different concentrations of medium-dissolved melamine were incubated for 24 h
with Caco-2 cells and tested for its anti-proliferative potential using MTT
assay. Half-maximal inhibitory concentration (IC₅₀) of melamine was
calculated from dose-response curve and found to be 14636 µg/L in the cells.
The results demonstrated that the dose dependent melamine decreased cell
proliferation and direct anti-proliferative effect in Caco-2 cells. 

Keywords

Anti-proliferative, Caco-2, Melamine, MTT

Melaminin İnsan Kolon Adenokarsinom Hücreleri Üzerindeki Anti-Proliferatif Etkisi

Abstract

Melamin, öncelikle
plastik dengeleyici ve yangın geciktirici olarak kullanılan organik bir
kimyasaldır. Hayvansal yem ve süt ürünlerinin protein içeriğini suni şekilde
yükseltmek için kullanılmasıyla bebeklerde böbrek hastalıklarına ve hayvan
ölümlerine yol açmıştır. Bu çalışmada, melaminin insan kolon adenokarsinom
hücrelerinin (Caco-2) proliferasyonu üzerine etkileri araştırılmıştır. Farklı
konsantrasyonlarda çözülmüş melamin Caco-2 hücreleri ile 24 saat inkübe edildi
ve MTT testi kullanılarak anti-proliferatif potansiyeli test edildi. Melaminin
yarı maksimal inhibisyon konsantrasyonu (IC₅₀), doz-cevap eğrisinden
hesaplandı ve hücrelerde 14636 µg/L olarak bulundu. Sonuçlar doza bağımlı
olarak melaminin Caco-2 hücrelerinin proliferasyonunu azalttığını ve Caco-2
hücrelerine karşı anti-proliferatif etkiye sahip olduğunu göstermiştir.

Keywords

Anti-proliferatif, Caco-2, Melamin, MTT

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