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The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow

Year 2021, , 82 - 87, 23.12.2021
https://doi.org/10.46897/livestockstudies.610206

Abstract

This study aimed to evaluate the superovulation response in Eastern Anatolian Red (EAR) cows treated with a single epidural injection or traditional administration of follicle stimulating hormone (FSH). Eight cows were randomly divided into two groups, as control (CG) and experiment (EG). Four replications were applied for each animal in the groups. A total of 16 studies were carried out in each group. In this study, 300 mg intramuscular (i.m) FSH injection was applied with decreasing doses twice daily for four days to the cows in the CG. (70:70, 50:50, 20:20, 10:10 mg). On the other hand, 300 mg FSH was administered as 150 mg epidural and 150 mg intramuscular injection to the cows in EG. The number of untransferable embryos and the total CLs were found statistically significant (p<0.05). However, the number of transferable embryos and unfertilized oocyte (UFO) were found statistically insignificant among the groups (p>0.05). According to the obtained data, it was observed that the epidural administration of FSH for superovulation in EAR cows did not provide sufficient follicle and oocyte development. In conclusion, different studies with different superovulation protocols and FSH doses in local breed EAR cows will be needed for further studies.

References

  • Alvarez, R.H., Martinez, A.C., Pires, R.M.L. (2010). Superovulatory response of Zebu cows treated with pFSH in a single subcutaneous injection followed by an additional intramuscular sub‐dose 48 h later. Reproduction in Domestic Animals, 45 (3):421-4. doi: 10.1111/j.1439-0531.2008.01209.x
  • Bailey, J.L., Blodeau, J.F., & Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon minireview. Copyrightq American Society of Andrology, 21(1):1-7. doi.org/10.1002/j.1939-4640.2000.tb03268.x.
  • Barati F, Niasari-Naslaji A, Bolourchi M, Sarhaddi F, Razavi K, Naghzali E, & Thatcher, W.W. (2006). Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology, 66 (5):1149-55. doi: 10.1016/j.theriogenology.2006.03.026.
  • Baruselli, P.S., de Sá Filho, M.F., Martins, C.M., Nasser, L.F., Nogueira, M.F.G., Barros, C.M., & Bó, G.A. (2006): Superovulation and embryo transfer in Bos indicus cattle. Theriogenology, 65 (1): 77-88. doi: 10.1016/j.theriogenology.2005.10.006.
  • Baylor, N.W., Egan, W., & Richman, P. (2002). Aluminum salts in vaccines-US perspective. Vaccine, 20:S18-23. doi: 10.1016/s0264-410x(02)00166-4.
  • Bó, G.A., Baruselli, P.S., Moreno, D., Cutaia, L., Caccia, M., Tribulo, R., Tríbulo, H., Mapletoft, R.J. (2002). The control of follicular wave development for self-appointed embryo transfer programs in cattle. Theriogenology, 57 (1): 53-72. doi: 10.1016/s0093-691x(01)00657-4.
  • Bó, G.A., Baruselli, P.S., Martınez, M.F. (2003): Pattern and manipulation of follicular development in Bos indicus cattle. Animal Reproduction Science, 78 (3): 307-326. doi: 10.1016/s0378-4320(03)00097-6.
  • Bó, G.A., & Mapletoft, R.J. (2014). Historical perspectives and recent research on superovulation in cattle. Theriogenology, 81(1):38-48. doi: 10.1016/j.theriogenology.2013.09.020.
  • Bó, G.A., Guerrero, D.C., Tríbulo, A., Tríbulo, H., Tríbulo, R., Rogan, D., Mapletoft, R.J. (2010). New approaches to superovulation in the cow. Reproduction, Fertility and Development, 22(1):106-12. doi: 10.1071/RD09226.
  • Bó, G.A., Adams, G.P., Pierson, R.A., & Mapletoft, R.J. (1996): Effect of progestogen plus E-17b treatment on superovulatory response in beef cattle. Theriogenology, 45(5): 897–910. doi: 10.1016/0093-691x(96)00020-9.
  • Carvalho, J.B.P. (2004). Synchronization of ovulation with intravaginal with progesterone device in Bos indicus, Bos indicus x Bos taurusand Bos taurus heifers. Ph.D. Thesis, University of SãoPaulo, Brasil, 2004.
  • Hasler, J.F. (2006). The Holstein cow in embryo transfer today as compared to 20 years ago. Theriogenology, 65 (1):4-16. doi: 10.1016/j.theriogenology.2005.10.002.
  • Hiemstra, S.J. (2004).Guidelines for the constitution of national cryopreservation programmes for farm animals. European Region Focal Point For Animal Genetic Resources. Available in: http://www.agrowebcee.net/ fileadmin/content/aw lithuania/files/Guidelinest.pdf.
  • Imai, K. (2005). Embryo Transfer Technology For Domestic Animal. Fucushima, Japan: Japan International Cooperation Agency National Livestock Breeding Center.
  • Kanitza, W., Beckera, F., Schneidera, F., Kanitzb, E., Leidingc, C., Nohnerc, H.P., & Pöhlanda, R. (2002). Superovulation in cattle: practical aspects of gonadotropin treatment and insemination. Reproduction Nutrition Development, 42 (6):587-99. doi: 10.1051/rnd:2002045.
  • Karaşahin, T., Kızıl, S.H., Satılmış, M., Akyol, N., Dursun, Ş., & Kırbaş, M (2016). Investigation of the response of different breeds to the superovulation treatment. JAVST; 1(1): 1-5.
  • Kaymaz, M., Fındık, M., Rişvanlı, A., & Köker, A. (2015). Obstetrics and Gynecology in Farm Animals. Medipres Matbaacılık Ltd. Şti., pp. 660-84. ISBN:978-605-9720-24-3.
  • Kelly, P., Duffy, P., Roche, J.F., & Boland, M.P. (1997). Superovulation in cattle: effect of FSH type and method of administration on follicular growth, ovulatory response and endocrine patterns. Animal Reproduction Science, 46 (1-2):1-14. doi: 10.1016/s0378-4320(96)01589-8.
  • Kim, I.H., Son, D.S., Yeon, S.H., Choi, S.H., Park, S.B., Ryu, I.S., Suh, G.H., Lee, D.W., Lee, C. S., Lee, H. J., & Yoon, J.T. (2000). Effect of dominant follicle removal before superstimulation of follicular growth, ovulation and embryo production in Holstein cows. Theriogenology, 55 (4):937-45. doi: 10.1016/s0093-691x(01)00455-1.
  • Kimura, K., Hirako, M., Iwata, H., Aoki, M., & Kawaguchi, M. Seki M. (2007). Successful superovulation of cattle by a single administration of FSH in aluminum hydroxide gel. Theriogenology, 68(4):633-9.doi: 10.1016/j.theriogenology.2007.02.016.
  • Kimura, K. (2016). Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle. Journal of Reproduction and Development, 62(5):423-9. doi: 10.1262/jrd.2016-066.
  • Lindblad, E.B. (2004). Aluminium adjuvants-in retrospect and prospect. Vaccine, 22 (27-28):3658-68. doi: 10.1016/j.vaccine.2004.03.032.
  • Lopes, A.S., Butler, S.T., Gilbert, R.O., & Butler, W. (2007). Relationship of pre-ovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Animal reproduction science, 99 (1-2):34-43. doi: 10.1016/j.anireprosci.2006.04.056.
  • Machaty, Z., Peippo, J., & Peter, A. (2012). Production and manipulation of bovine embryos: techniques and terminology. Theriogenology, 78 (5):937-50. doi: 10.1016/j.theriogenology.
  • Mapletoft, R.J., Steward, K.B., & Adams, G.P. (2002). Recent advances in the superovulation in cattle. Reproduction Nutrition Development, 42 (6):601-11. doi: 10.1051/rnd:2002046.
  • Medeiros C.M.O., Forell, F., Oliveira, A.T.D., & Rodrigues, J.L. (2002). Current status of sperm cryopreservation: why isn't it better? Theriogenology, 57(1):327- 44. doi: 10.1016/s0093-691x(01)00674-4.
  • Monniaux, D., Chupin, D., & Saumande, J. (1983). Superovulatory responses of cattle. Theriogenology, 19 (1):55-81. doi.org/10.1016/0093-691X(83)90124-3.
  • Taşdemir, U., Satılmış, M., Karaşahin, T., Kızıl, S.H., Kaymaz, M., & Imai, K. (2012). The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow. Veterinary Journal of Ankara University, 59:211-216. doi: 10.1501/ Vetfak.
  • Taşdemir, U., Karaşahin, T., Satılmış, M., Kızıl, S.H., & Kaymaz, M (2016). Various FSH administration on superovulatory response and embryo yield in Anatolian Black heifers. Kocatepe Vet J; 9 (4): 322-326
  • Tríbulo, A., Rogan, D., Tribulo, H., Tribulo, R., Alasino, R.V., Beltramo, D., Bianco, I., Mapletoft, R.J., & Bó, G.A. (2011). Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V. Animal Reproduction Science, 129 (1-2):7-13. doi: 10.1016/j.anireprosci.2011.10.013.
  • Tríbulo, A., Rogan, D., Tríbulo, H., Tríbulo, R., Mapletoft, R.J., & Bó, G.A. (2012). Superovulation of beef cattle with a split-single intramuscular administration of Folltropin-V in two concentrations of hyaluronan. Theriogenology, 77(8):1679-85. doi:10.1016/j.theriogenology.2011.12.013.
  • Sağırkaya, H. (2009). Embryo transfer in cattle and the importance of embryo transfer for Turkey. Uludag University Journal of Research in Veterinary Medicine, 28 (2): 11-19.
  • Satılmış, M., Akyol, N., Kızıl, S.H., & Karaşahin, T (2012). The investigation of superovulation responses with different protocols in Anatolian Native Black heifers. Lalahan Hay. Araşt. Enst. Derg. 57: (1) 13-17. http://arastirma.tarim.gov.tr/lalahan.
  • Sugano, M., & Watanabe S. (1997). Use of highly purified porcine FSH preparation for superovulation in Japanese black cattle. Journal of Veterinary Medical Science, 59 (3):223-5. doi: 10.1292/jvms.59.223.
  • Sugano, M., Shinogi, T., Nakada, K., & Nakao, T. (2001). Endocrine profiles and embryo quality in Japanese black cattle superovulated with human menopausal gonadotrophin and porcine follicle stimulating hormone. Reproduction in Domestic Animals, 36 (2):57-63. doi: 10.1046/j.1439-0531.2001.00192.x.
  • Son, D.S., Choe, C.Y., Choi, S.H., Cho, S.R., Kim, H.J., Han, M.H., Ryu, I.S., Suh, G.H., Kim, U. H., Kim, I.H. (2007). Effect of estradiol benzoate or GnRH treatment prior to superstimulation in CIDR-treated, Korean native cows (Bos taurus). Animal Reproduction Science, 100(1-2):14-21. doi: 10.1016/j.anireprosci.2006.06.005.
  • Yamamoto, M., Ooe, M., Kawaguchi, M., & Suzuki, T. (1994). Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone. Theriogenology, 41(3):747-55. doi: 10.1016/0093-691x(94)90184-k.
  • Yu, S., Ju, X.H., Wang, L.B., Fan, & J.F. (2007). Successful embryo transfer in Tianzhu white yak using standard protocol. Science in China Series C Life Sciences 50 (5):655-9. doi: 10.1007/s11427-007-0062-9.
Year 2021, , 82 - 87, 23.12.2021
https://doi.org/10.46897/livestockstudies.610206

Abstract

References

  • Alvarez, R.H., Martinez, A.C., Pires, R.M.L. (2010). Superovulatory response of Zebu cows treated with pFSH in a single subcutaneous injection followed by an additional intramuscular sub‐dose 48 h later. Reproduction in Domestic Animals, 45 (3):421-4. doi: 10.1111/j.1439-0531.2008.01209.x
  • Bailey, J.L., Blodeau, J.F., & Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon minireview. Copyrightq American Society of Andrology, 21(1):1-7. doi.org/10.1002/j.1939-4640.2000.tb03268.x.
  • Barati F, Niasari-Naslaji A, Bolourchi M, Sarhaddi F, Razavi K, Naghzali E, & Thatcher, W.W. (2006). Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology, 66 (5):1149-55. doi: 10.1016/j.theriogenology.2006.03.026.
  • Baruselli, P.S., de Sá Filho, M.F., Martins, C.M., Nasser, L.F., Nogueira, M.F.G., Barros, C.M., & Bó, G.A. (2006): Superovulation and embryo transfer in Bos indicus cattle. Theriogenology, 65 (1): 77-88. doi: 10.1016/j.theriogenology.2005.10.006.
  • Baylor, N.W., Egan, W., & Richman, P. (2002). Aluminum salts in vaccines-US perspective. Vaccine, 20:S18-23. doi: 10.1016/s0264-410x(02)00166-4.
  • Bó, G.A., Baruselli, P.S., Moreno, D., Cutaia, L., Caccia, M., Tribulo, R., Tríbulo, H., Mapletoft, R.J. (2002). The control of follicular wave development for self-appointed embryo transfer programs in cattle. Theriogenology, 57 (1): 53-72. doi: 10.1016/s0093-691x(01)00657-4.
  • Bó, G.A., Baruselli, P.S., Martınez, M.F. (2003): Pattern and manipulation of follicular development in Bos indicus cattle. Animal Reproduction Science, 78 (3): 307-326. doi: 10.1016/s0378-4320(03)00097-6.
  • Bó, G.A., & Mapletoft, R.J. (2014). Historical perspectives and recent research on superovulation in cattle. Theriogenology, 81(1):38-48. doi: 10.1016/j.theriogenology.2013.09.020.
  • Bó, G.A., Guerrero, D.C., Tríbulo, A., Tríbulo, H., Tríbulo, R., Rogan, D., Mapletoft, R.J. (2010). New approaches to superovulation in the cow. Reproduction, Fertility and Development, 22(1):106-12. doi: 10.1071/RD09226.
  • Bó, G.A., Adams, G.P., Pierson, R.A., & Mapletoft, R.J. (1996): Effect of progestogen plus E-17b treatment on superovulatory response in beef cattle. Theriogenology, 45(5): 897–910. doi: 10.1016/0093-691x(96)00020-9.
  • Carvalho, J.B.P. (2004). Synchronization of ovulation with intravaginal with progesterone device in Bos indicus, Bos indicus x Bos taurusand Bos taurus heifers. Ph.D. Thesis, University of SãoPaulo, Brasil, 2004.
  • Hasler, J.F. (2006). The Holstein cow in embryo transfer today as compared to 20 years ago. Theriogenology, 65 (1):4-16. doi: 10.1016/j.theriogenology.2005.10.002.
  • Hiemstra, S.J. (2004).Guidelines for the constitution of national cryopreservation programmes for farm animals. European Region Focal Point For Animal Genetic Resources. Available in: http://www.agrowebcee.net/ fileadmin/content/aw lithuania/files/Guidelinest.pdf.
  • Imai, K. (2005). Embryo Transfer Technology For Domestic Animal. Fucushima, Japan: Japan International Cooperation Agency National Livestock Breeding Center.
  • Kanitza, W., Beckera, F., Schneidera, F., Kanitzb, E., Leidingc, C., Nohnerc, H.P., & Pöhlanda, R. (2002). Superovulation in cattle: practical aspects of gonadotropin treatment and insemination. Reproduction Nutrition Development, 42 (6):587-99. doi: 10.1051/rnd:2002045.
  • Karaşahin, T., Kızıl, S.H., Satılmış, M., Akyol, N., Dursun, Ş., & Kırbaş, M (2016). Investigation of the response of different breeds to the superovulation treatment. JAVST; 1(1): 1-5.
  • Kaymaz, M., Fındık, M., Rişvanlı, A., & Köker, A. (2015). Obstetrics and Gynecology in Farm Animals. Medipres Matbaacılık Ltd. Şti., pp. 660-84. ISBN:978-605-9720-24-3.
  • Kelly, P., Duffy, P., Roche, J.F., & Boland, M.P. (1997). Superovulation in cattle: effect of FSH type and method of administration on follicular growth, ovulatory response and endocrine patterns. Animal Reproduction Science, 46 (1-2):1-14. doi: 10.1016/s0378-4320(96)01589-8.
  • Kim, I.H., Son, D.S., Yeon, S.H., Choi, S.H., Park, S.B., Ryu, I.S., Suh, G.H., Lee, D.W., Lee, C. S., Lee, H. J., & Yoon, J.T. (2000). Effect of dominant follicle removal before superstimulation of follicular growth, ovulation and embryo production in Holstein cows. Theriogenology, 55 (4):937-45. doi: 10.1016/s0093-691x(01)00455-1.
  • Kimura, K., Hirako, M., Iwata, H., Aoki, M., & Kawaguchi, M. Seki M. (2007). Successful superovulation of cattle by a single administration of FSH in aluminum hydroxide gel. Theriogenology, 68(4):633-9.doi: 10.1016/j.theriogenology.2007.02.016.
  • Kimura, K. (2016). Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle. Journal of Reproduction and Development, 62(5):423-9. doi: 10.1262/jrd.2016-066.
  • Lindblad, E.B. (2004). Aluminium adjuvants-in retrospect and prospect. Vaccine, 22 (27-28):3658-68. doi: 10.1016/j.vaccine.2004.03.032.
  • Lopes, A.S., Butler, S.T., Gilbert, R.O., & Butler, W. (2007). Relationship of pre-ovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Animal reproduction science, 99 (1-2):34-43. doi: 10.1016/j.anireprosci.2006.04.056.
  • Machaty, Z., Peippo, J., & Peter, A. (2012). Production and manipulation of bovine embryos: techniques and terminology. Theriogenology, 78 (5):937-50. doi: 10.1016/j.theriogenology.
  • Mapletoft, R.J., Steward, K.B., & Adams, G.P. (2002). Recent advances in the superovulation in cattle. Reproduction Nutrition Development, 42 (6):601-11. doi: 10.1051/rnd:2002046.
  • Medeiros C.M.O., Forell, F., Oliveira, A.T.D., & Rodrigues, J.L. (2002). Current status of sperm cryopreservation: why isn't it better? Theriogenology, 57(1):327- 44. doi: 10.1016/s0093-691x(01)00674-4.
  • Monniaux, D., Chupin, D., & Saumande, J. (1983). Superovulatory responses of cattle. Theriogenology, 19 (1):55-81. doi.org/10.1016/0093-691X(83)90124-3.
  • Taşdemir, U., Satılmış, M., Karaşahin, T., Kızıl, S.H., Kaymaz, M., & Imai, K. (2012). The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow. Veterinary Journal of Ankara University, 59:211-216. doi: 10.1501/ Vetfak.
  • Taşdemir, U., Karaşahin, T., Satılmış, M., Kızıl, S.H., & Kaymaz, M (2016). Various FSH administration on superovulatory response and embryo yield in Anatolian Black heifers. Kocatepe Vet J; 9 (4): 322-326
  • Tríbulo, A., Rogan, D., Tribulo, H., Tribulo, R., Alasino, R.V., Beltramo, D., Bianco, I., Mapletoft, R.J., & Bó, G.A. (2011). Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V. Animal Reproduction Science, 129 (1-2):7-13. doi: 10.1016/j.anireprosci.2011.10.013.
  • Tríbulo, A., Rogan, D., Tríbulo, H., Tríbulo, R., Mapletoft, R.J., & Bó, G.A. (2012). Superovulation of beef cattle with a split-single intramuscular administration of Folltropin-V in two concentrations of hyaluronan. Theriogenology, 77(8):1679-85. doi:10.1016/j.theriogenology.2011.12.013.
  • Sağırkaya, H. (2009). Embryo transfer in cattle and the importance of embryo transfer for Turkey. Uludag University Journal of Research in Veterinary Medicine, 28 (2): 11-19.
  • Satılmış, M., Akyol, N., Kızıl, S.H., & Karaşahin, T (2012). The investigation of superovulation responses with different protocols in Anatolian Native Black heifers. Lalahan Hay. Araşt. Enst. Derg. 57: (1) 13-17. http://arastirma.tarim.gov.tr/lalahan.
  • Sugano, M., & Watanabe S. (1997). Use of highly purified porcine FSH preparation for superovulation in Japanese black cattle. Journal of Veterinary Medical Science, 59 (3):223-5. doi: 10.1292/jvms.59.223.
  • Sugano, M., Shinogi, T., Nakada, K., & Nakao, T. (2001). Endocrine profiles and embryo quality in Japanese black cattle superovulated with human menopausal gonadotrophin and porcine follicle stimulating hormone. Reproduction in Domestic Animals, 36 (2):57-63. doi: 10.1046/j.1439-0531.2001.00192.x.
  • Son, D.S., Choe, C.Y., Choi, S.H., Cho, S.R., Kim, H.J., Han, M.H., Ryu, I.S., Suh, G.H., Kim, U. H., Kim, I.H. (2007). Effect of estradiol benzoate or GnRH treatment prior to superstimulation in CIDR-treated, Korean native cows (Bos taurus). Animal Reproduction Science, 100(1-2):14-21. doi: 10.1016/j.anireprosci.2006.06.005.
  • Yamamoto, M., Ooe, M., Kawaguchi, M., & Suzuki, T. (1994). Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone. Theriogenology, 41(3):747-55. doi: 10.1016/0093-691x(94)90184-k.
  • Yu, S., Ju, X.H., Wang, L.B., Fan, & J.F. (2007). Successful embryo transfer in Tianzhu white yak using standard protocol. Science in China Series C Life Sciences 50 (5):655-9. doi: 10.1007/s11427-007-0062-9.
There are 38 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section 61-2
Authors

Muharrem Satılmış This is me

Mehmet Ali Yılmaz This is me

Ramazan Sevgi This is me

Tahir Karaşahin

Sedat Hamdi Kızıl This is me

Alaeddin Okuroğlu This is me

Talha Burak Ertem This is me

Zulal Tavlı Yıldırır This is me

Publication Date December 23, 2021
Published in Issue Year 2021

Cite

APA Satılmış, M., Yılmaz, M. A., Sevgi, R., Karaşahin, T., et al. (2021). The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow. Livestock Studies, 61(2), 82-87. https://doi.org/10.46897/livestockstudies.610206
AMA Satılmış M, Yılmaz MA, Sevgi R, Karaşahin T, Kızıl SH, Okuroğlu A, Ertem TB, Tavlı Yıldırır Z. The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow. Livestock Studies. December 2021;61(2):82-87. doi:10.46897/livestockstudies.610206
Chicago Satılmış, Muharrem, Mehmet Ali Yılmaz, Ramazan Sevgi, Tahir Karaşahin, Sedat Hamdi Kızıl, Alaeddin Okuroğlu, Talha Burak Ertem, and Zulal Tavlı Yıldırır. “The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow”. Livestock Studies 61, no. 2 (December 2021): 82-87. https://doi.org/10.46897/livestockstudies.610206.
EndNote Satılmış M, Yılmaz MA, Sevgi R, Karaşahin T, Kızıl SH, Okuroğlu A, Ertem TB, Tavlı Yıldırır Z (December 1, 2021) The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow. Livestock Studies 61 2 82–87.
IEEE M. Satılmış, M. A. Yılmaz, R. Sevgi, T. Karaşahin, S. H. Kızıl, A. Okuroğlu, T. B. Ertem, and Z. Tavlı Yıldırır, “The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow”, Livestock Studies, vol. 61, no. 2, pp. 82–87, 2021, doi: 10.46897/livestockstudies.610206.
ISNAD Satılmış, Muharrem et al. “The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow”. Livestock Studies 61/2 (December 2021), 82-87. https://doi.org/10.46897/livestockstudies.610206.
JAMA Satılmış M, Yılmaz MA, Sevgi R, Karaşahin T, Kızıl SH, Okuroğlu A, Ertem TB, Tavlı Yıldırır Z. The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow. Livestock Studies. 2021;61:82–87.
MLA Satılmış, Muharrem et al. “The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow”. Livestock Studies, vol. 61, no. 2, 2021, pp. 82-87, doi:10.46897/livestockstudies.610206.
Vancouver Satılmış M, Yılmaz MA, Sevgi R, Karaşahin T, Kızıl SH, Okuroğlu A, Ertem TB, Tavlı Yıldırır Z. The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow. Livestock Studies. 2021;61(2):82-7.