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First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo

Year 2021, Volume: 61 Issue: 2, 60 - 65, 23.12.2021
https://doi.org/10.46897/livestockstudies.610203

Abstract

Conventional buffalo semen freezing studies are limited in Anatolian buffaloes, which are overly sensitive to exogenous stimulation. The present study's object was to determine the main features of Anatolian buffalo semen obtained by artificial vagina method for the first time. A total number of 150 ejaculates were collected from three Anatolian Buffalo bulls (app. 4 years of age). The mean pH, volume and concentration of semen were found 6.63±0.15, 1.61±0.5 ml, 1629±222.67 x106 spermatozoa/ml, respectively. The sperm motion characteristics were determined by using a computer-assisted sperm analysis system (CASA); the total and progressively motile sperm values were 57.12±5.63%, 23.22±4.47% and other kinetic parameters such VAP, VSL, VCL, ALH, BCF, STR, LIN were found 94.71±8.48 µm/s, 72.6±7.08 µm/s, 160.9±15.66 µm/s, 7.8±3.75 µm, 29.15±1.56 Hz, 76.91±3.87%, 46.21±2.61%, respectively after thawing. Among buffalo bulls, differences in semen pH values were statistically significant (P>0.05), while differences in ejaculate volume, semen concentration, total motility, progressive motility, VAP, VSL, VCL, ALH, BCF, STR, and LIN were not (P>0.05). As a result, frozen Anatolian buffalo semen can be obtained economically and can be used for animal breeding in assisted reproductive biotechnology such as artificial insemination or in vitro embryo production commercially.

References

  • Ahmed, A., Ayaz, M., Mughal, D.H., Rehman, A., Mushtaq, M.H., Yousaf, M.S., Riaz, A. (2020). Anti-oxidative effect of l-lysine on post thaw quality of Nili-Ravi buffalo bull semen (Bubalus bubalis).
  • Journal of Animal and Plant Sciences, 30:612-618. https://doi.org/10.36899 /JAPS.2020.3.0072
  • Aköz, M., Arik, D., Kul, M., Çelik, B. (2017). Buffalo breeding: Buffalo breeding in Turkey from past to today. Journal of Scientific and Technological Research, 3: 2422-8702.
  • Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalis bubalis) bull spermatozoa. Reproduction in Domestic Animals, 44: 552-569. https://doi.org/10.1111/j.1439-0531.2008.01240.x.
  • Ansari, M.S., Rakha, B.A., Akhter S. (2017). Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation. Reproduction in Domestic Animals, 52: 992-997. https://doi.org/10.1111/rda.13008.
  • Arat, S. (2011). In vitro conservation and preliminary molecular identification of some Turkish domestic animal genetic resources (TURKHAYGEN-I). In Proceedings of the 8th Global Conference on the Conservation of Animal Genetic Resources, Turkey. p.51-58.
  • Atasever, S., Erdem, H. (2008). Water buffalo raising and its future in Turkey. Anadolu Journal of Agricultural Sciences, 23(1): 59-64.
  • Baştan, İ., Korkmaz, F., Şahin, D., Yırtıcı, S., Kinet, H., Satılmış, M., Tekerli, M. (2018). First Commercial Production and Application of Cryopreserved Anatolian Buffalo Sperm. In Proceedings of the 9th National Reproduction and Artificial Insemination Science Congress, Turkey. p:126-127.
  • Baştan, İ. (2020). Controlled semen collection system for artificial insemination from Anatolian buffaloes. Turkish Patent and Trademark Office. Application Number of Patent: 2020/11130.
  • Bhakat, M., Mohanty, T.K., Raina, V.S., Gupta, A.K., Khan, H.M. (2011). Frozen semen production performance of Murrah buffalo bulls. Buffalo Bulletin. 30: 157-164.
  • Çolak, M., Javorka, L. (2017). Hungarian Expert Oszkár Wellmann’s Report in the Establishment Years of the Republic of Turkey. 1st ed. p.115-155.
  • Dhami, A.J., Shelke, V.B. (2005). Investigations into the causes of poor semen quality and freezability in Jafarabadi buffalo bulls. The Indian Journal of Animal Sciences. 75: 925-929.
  • Galli, A., Bornaghi, V., Balduzzi, D., Buttazzoni, L., Aleandri, R. (1993). Sexual behaviour and semen quality relating to Italian Buffalo. Proceedings 3rd World Buffalo Congress, Varna, Bulgaria. pp.562–570.
  • Gaviraghi, A., Puglisi, R., Balduzzi, D., Severgnini, A., Bornaghi, V., Bongioni, G., Galli, A. (2013). Minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology, 79(8),1171-1176. https://doi:/10.1016/ j.theriogenology.2013.02.014.
  • Ghodasara, S.N., Gajbhiye, P.U., Ahlawat, A.R., Murthy, K.S. (2018). Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull. Buffalo Bulletin. 35(1): 65-72.
  • Işık, M. (2015). Economic analysis of water buffalo farming in Mus province, Turkey. M.Sc. Süleyman Demirel University Isparta, Turkey.
  • İnanç, M,E., Çil, B., Tekin, K., Alemdar, H., Daşkin, A. (2018). The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen. Turkish Journal of Veterinary and Animal Sciences. 42: 452-458. https://doi:10.3906/vet-1801-83.
  • Jainudeen, M.R., Bongso, T.A., Dass, S. (1982). Semen characteristics of the swamp buffalo (Bubalus bubalis). Animal Reproduction Science. 4: 213–217. https://doi:10.1016/0378-4320(82)90005-7.
  • Kaka, A., Samo, U., Rahoo, T.H., Rehman, Z.U., Shah, Z., Mushtag, M., Behan, A.A. (2012). Study on post thawing quality of Kundhi buffalo semen. Journal of Animal and Plant Sciences. 22(2 Supplement), 59-62.
  • Kelgökmen, İ. (2015). Some morphometric traits of Anatolian Buffaloes. Livestock Studies. 55: 50-55.
  • Khawaskar, M.V., Panchal, M.T., Dhami, A.J., Hadiya, K.K., Patel, S.B. (2012). Seasonal variation in seminal biochemical constituents in Surti buffalo bulls. Indian Journal of Animal Reproduction, 33: 41-46.
  • Korkmaz, F., Baştan, İ., Şahin, D., Yırtıcı, S. (2019). Lalahan model in commercial Anatolian buffalo semen production. Proceedings 12th World Buffalo Congress, İstanbul, Turkey. p.44.
  • Kumar, D., Kumar, P., Yadav, P.S. (2016). Quantitative evaluation of buffalo semen by CASA during cryopreservation process. The Indian Journal of Animal Reproduction. 37: 15-18.
  • Mahmoud, K.G.M., El Sokary, A.A.E., Abou El Roos, M.E.A., Nawito, M. (2013). Sperm characteristics in cryopreserved buffalo bull semen and field fertility. Iranian Journal of Applied Animal Science. 3: 777-783.
  • Malik, M,A., Ahmad, M., Gilani, H., Chaudhry, R.A. (1974). Studies on the morphology and abnormalities of buffalo spermatozoa. Pakistan Journal of Agricultural Research. 11: 41-46.
  • Morrell, J.M. (2011). Artificial insemination: current and future trends. In: Manafi M (editor). Artificial Insemination In Farm Animals. Rijeka, Croatia. p.1-14.
  • Murphy, E.M., Kelly, A.K., O’Meara, C., Eivers, B., Lonergan, P., Fair, S. (2018). Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination centre. Journal of Animal Science, 96: 2408-2418. https://doi: /10.1093/jas/sky130.
  • Okuyucu, İ.C., Bayyurt, L., Akdağ, A., Trink, C., Bulu, A. (2018). Evaluation of Enterprises Raising Water Buffalo in Samsun Province of Turkey. International Journal of Scientific and Technological Research, 4(8): 1-5.
  • Pathak, P.K., Dhami, A.J., Chaudhari, D.V., Hadiya, K.K. (2020). Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA. Indian Journal of Animal Research, 54: 1188-1194.
  • Roy, A., Srivastava, R.K., Pandey, M.D. (1956). Deep freezing of buffalo semen diluted and preserved in glycine – egg yolk medium. Indian Journal of Dairy Science, 9: 61-62.
  • Sankhi, S., Sapkota, K.R., Regmi, B. (2019). Effect of age and frequency of collection on quality of Jersey bulls semen at National Livestock Breeding Center (NLBC), Nepal. International Journal of Applied Sciences and Biotechnology, 7: 88-95. https//doi: /10.3126/ijasbt.v7i1.23312.
  • Selcuk, M., Akal, E. (2015). Testicular morphology and in vitro evaluation of frozen epididymal sperm of Anatolian buffalo. Veterinary Journal of Ankara University. 62: 51-55. https//doi:/10.1501/Vetfak_0000002657.
  • Singh, A.K., Kumar, A., Honparkhe, M., Kaur, S., Kaur, H., Ghuman, S.P.S., Brar, P.S. (2018). Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk, soya bean lecithin‐and liposome‐based extenders. Reproduction in Domestic Animals, 53: 195-202. https//doi:/10.1111/rda.13092.
  • Singh, R.K., Kumaresan, A., Mir, M.A., Kumar, P., Chhillar, S., Tripathi, U.K., Mohanty, T.K. (2017). Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility. Indian Journal of Animal Research, 51: 660-664.
  • Soysal, M., Ozkan, E., Kok, S., Occidente, M., Tuna, Y.T., Gurcan, E.K., Matassino, D. (2007). Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers. Italian Journal of Animal Science. 6: 409-412. https//doi.org/10.4081/ijas.2007.s2.409.
  • Soysal, M., Ünal, E., Gürcan, E. (2020). Conservation and Sustainable Use of Farm Animal Genetic Resource. Journal of Animal Science and Products. 3: 210-227.
  • Şahi̇n, D., Baştan, İ., Çi̇l, B., Teki̇n, K., Akçay, E., Daskın, A., Stelletta, C. (2020). The number of false mounting affects the quality of semen in bulls. Livestock Studies. 60: 9-14. https//doi:/10.46897/lahaed.701388.
  • Tuncer, P.B., Bucak, M.N., Büyükleblebici, S., Sarıözkan, S., Yeni, D., Eken, A., Gündoğan, M. (2010). The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen. Cryobiology. 61: 303-307. https//doi.org/10.1016/j.cryobiol.2010.09.009.
  • Vincent, P., Underwood, S.L., Dolbec, C., Bouchard, N., Kroetsch, T., Blondin, P. (2018). Bovine semen quality control in artificial insemination centers. Animal Reproduction. 9: 153-165
  • Yeni, D., Avdatek, F. (2017). Effect of carnosic acid on the short term storage anatolian buffalo epididymal sperm. Kocatepe Veterinary Journal. 10: 187-195. https//doi:10.5578/kvj.58659.
  • Yılmaz, S. (2013). Water buffalo breeding in Afyonkarahisar province; sample of Küçükçobanli Village. M.Sc. Adnan Menderes University, Aydın, Turkey.
Year 2021, Volume: 61 Issue: 2, 60 - 65, 23.12.2021
https://doi.org/10.46897/livestockstudies.610203

Abstract

References

  • Ahmed, A., Ayaz, M., Mughal, D.H., Rehman, A., Mushtaq, M.H., Yousaf, M.S., Riaz, A. (2020). Anti-oxidative effect of l-lysine on post thaw quality of Nili-Ravi buffalo bull semen (Bubalus bubalis).
  • Journal of Animal and Plant Sciences, 30:612-618. https://doi.org/10.36899 /JAPS.2020.3.0072
  • Aköz, M., Arik, D., Kul, M., Çelik, B. (2017). Buffalo breeding: Buffalo breeding in Turkey from past to today. Journal of Scientific and Technological Research, 3: 2422-8702.
  • Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalis bubalis) bull spermatozoa. Reproduction in Domestic Animals, 44: 552-569. https://doi.org/10.1111/j.1439-0531.2008.01240.x.
  • Ansari, M.S., Rakha, B.A., Akhter S. (2017). Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation. Reproduction in Domestic Animals, 52: 992-997. https://doi.org/10.1111/rda.13008.
  • Arat, S. (2011). In vitro conservation and preliminary molecular identification of some Turkish domestic animal genetic resources (TURKHAYGEN-I). In Proceedings of the 8th Global Conference on the Conservation of Animal Genetic Resources, Turkey. p.51-58.
  • Atasever, S., Erdem, H. (2008). Water buffalo raising and its future in Turkey. Anadolu Journal of Agricultural Sciences, 23(1): 59-64.
  • Baştan, İ., Korkmaz, F., Şahin, D., Yırtıcı, S., Kinet, H., Satılmış, M., Tekerli, M. (2018). First Commercial Production and Application of Cryopreserved Anatolian Buffalo Sperm. In Proceedings of the 9th National Reproduction and Artificial Insemination Science Congress, Turkey. p:126-127.
  • Baştan, İ. (2020). Controlled semen collection system for artificial insemination from Anatolian buffaloes. Turkish Patent and Trademark Office. Application Number of Patent: 2020/11130.
  • Bhakat, M., Mohanty, T.K., Raina, V.S., Gupta, A.K., Khan, H.M. (2011). Frozen semen production performance of Murrah buffalo bulls. Buffalo Bulletin. 30: 157-164.
  • Çolak, M., Javorka, L. (2017). Hungarian Expert Oszkár Wellmann’s Report in the Establishment Years of the Republic of Turkey. 1st ed. p.115-155.
  • Dhami, A.J., Shelke, V.B. (2005). Investigations into the causes of poor semen quality and freezability in Jafarabadi buffalo bulls. The Indian Journal of Animal Sciences. 75: 925-929.
  • Galli, A., Bornaghi, V., Balduzzi, D., Buttazzoni, L., Aleandri, R. (1993). Sexual behaviour and semen quality relating to Italian Buffalo. Proceedings 3rd World Buffalo Congress, Varna, Bulgaria. pp.562–570.
  • Gaviraghi, A., Puglisi, R., Balduzzi, D., Severgnini, A., Bornaghi, V., Bongioni, G., Galli, A. (2013). Minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology, 79(8),1171-1176. https://doi:/10.1016/ j.theriogenology.2013.02.014.
  • Ghodasara, S.N., Gajbhiye, P.U., Ahlawat, A.R., Murthy, K.S. (2018). Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull. Buffalo Bulletin. 35(1): 65-72.
  • Işık, M. (2015). Economic analysis of water buffalo farming in Mus province, Turkey. M.Sc. Süleyman Demirel University Isparta, Turkey.
  • İnanç, M,E., Çil, B., Tekin, K., Alemdar, H., Daşkin, A. (2018). The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen. Turkish Journal of Veterinary and Animal Sciences. 42: 452-458. https://doi:10.3906/vet-1801-83.
  • Jainudeen, M.R., Bongso, T.A., Dass, S. (1982). Semen characteristics of the swamp buffalo (Bubalus bubalis). Animal Reproduction Science. 4: 213–217. https://doi:10.1016/0378-4320(82)90005-7.
  • Kaka, A., Samo, U., Rahoo, T.H., Rehman, Z.U., Shah, Z., Mushtag, M., Behan, A.A. (2012). Study on post thawing quality of Kundhi buffalo semen. Journal of Animal and Plant Sciences. 22(2 Supplement), 59-62.
  • Kelgökmen, İ. (2015). Some morphometric traits of Anatolian Buffaloes. Livestock Studies. 55: 50-55.
  • Khawaskar, M.V., Panchal, M.T., Dhami, A.J., Hadiya, K.K., Patel, S.B. (2012). Seasonal variation in seminal biochemical constituents in Surti buffalo bulls. Indian Journal of Animal Reproduction, 33: 41-46.
  • Korkmaz, F., Baştan, İ., Şahin, D., Yırtıcı, S. (2019). Lalahan model in commercial Anatolian buffalo semen production. Proceedings 12th World Buffalo Congress, İstanbul, Turkey. p.44.
  • Kumar, D., Kumar, P., Yadav, P.S. (2016). Quantitative evaluation of buffalo semen by CASA during cryopreservation process. The Indian Journal of Animal Reproduction. 37: 15-18.
  • Mahmoud, K.G.M., El Sokary, A.A.E., Abou El Roos, M.E.A., Nawito, M. (2013). Sperm characteristics in cryopreserved buffalo bull semen and field fertility. Iranian Journal of Applied Animal Science. 3: 777-783.
  • Malik, M,A., Ahmad, M., Gilani, H., Chaudhry, R.A. (1974). Studies on the morphology and abnormalities of buffalo spermatozoa. Pakistan Journal of Agricultural Research. 11: 41-46.
  • Morrell, J.M. (2011). Artificial insemination: current and future trends. In: Manafi M (editor). Artificial Insemination In Farm Animals. Rijeka, Croatia. p.1-14.
  • Murphy, E.M., Kelly, A.K., O’Meara, C., Eivers, B., Lonergan, P., Fair, S. (2018). Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination centre. Journal of Animal Science, 96: 2408-2418. https://doi: /10.1093/jas/sky130.
  • Okuyucu, İ.C., Bayyurt, L., Akdağ, A., Trink, C., Bulu, A. (2018). Evaluation of Enterprises Raising Water Buffalo in Samsun Province of Turkey. International Journal of Scientific and Technological Research, 4(8): 1-5.
  • Pathak, P.K., Dhami, A.J., Chaudhari, D.V., Hadiya, K.K. (2020). Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA. Indian Journal of Animal Research, 54: 1188-1194.
  • Roy, A., Srivastava, R.K., Pandey, M.D. (1956). Deep freezing of buffalo semen diluted and preserved in glycine – egg yolk medium. Indian Journal of Dairy Science, 9: 61-62.
  • Sankhi, S., Sapkota, K.R., Regmi, B. (2019). Effect of age and frequency of collection on quality of Jersey bulls semen at National Livestock Breeding Center (NLBC), Nepal. International Journal of Applied Sciences and Biotechnology, 7: 88-95. https//doi: /10.3126/ijasbt.v7i1.23312.
  • Selcuk, M., Akal, E. (2015). Testicular morphology and in vitro evaluation of frozen epididymal sperm of Anatolian buffalo. Veterinary Journal of Ankara University. 62: 51-55. https//doi:/10.1501/Vetfak_0000002657.
  • Singh, A.K., Kumar, A., Honparkhe, M., Kaur, S., Kaur, H., Ghuman, S.P.S., Brar, P.S. (2018). Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk, soya bean lecithin‐and liposome‐based extenders. Reproduction in Domestic Animals, 53: 195-202. https//doi:/10.1111/rda.13092.
  • Singh, R.K., Kumaresan, A., Mir, M.A., Kumar, P., Chhillar, S., Tripathi, U.K., Mohanty, T.K. (2017). Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility. Indian Journal of Animal Research, 51: 660-664.
  • Soysal, M., Ozkan, E., Kok, S., Occidente, M., Tuna, Y.T., Gurcan, E.K., Matassino, D. (2007). Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers. Italian Journal of Animal Science. 6: 409-412. https//doi.org/10.4081/ijas.2007.s2.409.
  • Soysal, M., Ünal, E., Gürcan, E. (2020). Conservation and Sustainable Use of Farm Animal Genetic Resource. Journal of Animal Science and Products. 3: 210-227.
  • Şahi̇n, D., Baştan, İ., Çi̇l, B., Teki̇n, K., Akçay, E., Daskın, A., Stelletta, C. (2020). The number of false mounting affects the quality of semen in bulls. Livestock Studies. 60: 9-14. https//doi:/10.46897/lahaed.701388.
  • Tuncer, P.B., Bucak, M.N., Büyükleblebici, S., Sarıözkan, S., Yeni, D., Eken, A., Gündoğan, M. (2010). The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen. Cryobiology. 61: 303-307. https//doi.org/10.1016/j.cryobiol.2010.09.009.
  • Vincent, P., Underwood, S.L., Dolbec, C., Bouchard, N., Kroetsch, T., Blondin, P. (2018). Bovine semen quality control in artificial insemination centers. Animal Reproduction. 9: 153-165
  • Yeni, D., Avdatek, F. (2017). Effect of carnosic acid on the short term storage anatolian buffalo epididymal sperm. Kocatepe Veterinary Journal. 10: 187-195. https//doi:10.5578/kvj.58659.
  • Yılmaz, S. (2013). Water buffalo breeding in Afyonkarahisar province; sample of Küçükçobanli Village. M.Sc. Adnan Menderes University, Aydın, Turkey.
There are 41 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section 61-2
Authors

İlktan Baştan This is me

Fırat Korkmaz This is me

Derya Şahin This is me

Seher Şimşek This is me

Muharrem Satılmış This is me

Publication Date December 23, 2021
Published in Issue Year 2021 Volume: 61 Issue: 2

Cite

APA Baştan, İ., Korkmaz, F., Şahin, D., Şimşek, S., et al. (2021). First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo. Livestock Studies, 61(2), 60-65. https://doi.org/10.46897/livestockstudies.610203
AMA Baştan İ, Korkmaz F, Şahin D, Şimşek S, Satılmış M. First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo. Livestock Studies. December 2021;61(2):60-65. doi:10.46897/livestockstudies.610203
Chicago Baştan, İlktan, Fırat Korkmaz, Derya Şahin, Seher Şimşek, and Muharrem Satılmış. “First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo”. Livestock Studies 61, no. 2 (December 2021): 60-65. https://doi.org/10.46897/livestockstudies.610203.
EndNote Baştan İ, Korkmaz F, Şahin D, Şimşek S, Satılmış M (December 1, 2021) First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo. Livestock Studies 61 2 60–65.
IEEE İ. Baştan, F. Korkmaz, D. Şahin, S. Şimşek, and M. Satılmış, “First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo”, Livestock Studies, vol. 61, no. 2, pp. 60–65, 2021, doi: 10.46897/livestockstudies.610203.
ISNAD Baştan, İlktan et al. “First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo”. Livestock Studies 61/2 (December 2021), 60-65. https://doi.org/10.46897/livestockstudies.610203.
JAMA Baştan İ, Korkmaz F, Şahin D, Şimşek S, Satılmış M. First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo. Livestock Studies. 2021;61:60–65.
MLA Baştan, İlktan et al. “First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo”. Livestock Studies, vol. 61, no. 2, 2021, pp. 60-65, doi:10.46897/livestockstudies.610203.
Vancouver Baştan İ, Korkmaz F, Şahin D, Şimşek S, Satılmış M. First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo. Livestock Studies. 2021;61(2):60-5.