Teke Spermasının Kriyokonservasyonu ve Uygun Yöntem Kullanımı

Year 2020, Volume: 1 Issue: 2, 118 - 129, 31.12.2020

Coşkun Konyalı

Abstract

Kriyokonservasyon, üreme biyoteknolojisi, ıslah ve genetik kaynakların korunma ve muhafazası gibi birçok alanda önemli fayda sağlamaktadır. Uygun kriyokonservasyon yöntemleri sayesinde üremede başarı arttırılabilecektir. Özellikle keçiler gibi mevsime bağlı üreme yapısına sahip türlerde kriyokonservasyon büyük avantaj sağlamaktadır. Türlere göre üreme biyoteknolojilerinin geliştirilmesi, sahip oldukları biyolojik farklılıkların bir sonucu olup, dondurma çözdürme sonrası hayatta kalma oranı türlere göre önemli farklılık göstermektedir. Sperma membranının lipid ve protein gibi kompozisyonunu oluşturan bileşenlerin çözdürme sonrası kalite üzerinde etkili olduğu bilinmektedir. Teke spermasının özelliklerinin belirlenerek uygun semen alımı, değerlendirilmesi işlemleri ile dondurma protokollerinin geliştirilmesi üreme performansını arttırıcı önemli unsurlar olup, yapay tohumlama ya da diğer in vitro – in vivo çalışmalarda spermin kullanılabilirliğini etkilemektedir. Kriyokonservasyonun zararlı ve öldürücü etkisinin azaltılması dondurma çözdürme sonrası sperm kalitesi üzerinde doğrudan etkilidir. Dondurmada kullanılan çözelti ve biyolojik ortamların iyileştirilmesi gerekmektedir. Bu bağlamda tekelerde semen alımı, işlenmesi, kriyokonservasyonu ve çözdürme süreçlerini kapsayan protokollerin maliyet, iş gücü ve zaman tasarrufu da sağlayarak uygun sperm analiz yöntemlerin oluşturulması gerekmektedir.

Keywords

CASA, üreme, kolesterol, sperm analiz, sperm kalite

References

• Bearden, H.J., Fuquay, J.W., Willard S.T. 2000. Applied Animal Reproduction. Pearson Prentice Hall, New Jersey.
• Chandler, J.E., Painter, C.L., Adkison, R.W., Memon, M.A., Hoyt P.G., 1988. Semen Quality Characteristics of Dairy Goats. Journal of Dairy Science.Vol. 71, No. 6. https://doi.org/10.3168/jds.S0022-0302(88)79728-3.
• Christian, A.E., Haynes, M.P., Phillips, M.C., Rothblat, G.H., 1997. Use of cyclodextrins for manipulating cellular cholesterol content. J. Lipid Res. 38 : 2264-2272.
• Combes, G.B., Varner, D.D., Schroeder, F., Burghardt, R.C., Blanchard, T.L., 2000. Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing. Journal of Reproduction and Fertility Suppplement 56, 127-132.
• Corteel, J.M., 1977. Production, storage and artificiel insemination of goat semen. Management of Reproduction in Sheep and Goats Symposium, Madison, July 24–25, s. 41–57.
• Curry, M.R., 2000. Cryopreservation of semen from domestic livestock. Rev. Reprod. 5:46–52.
• Delgadillo, J.A., 1990. Abolition des variations saisonnie`res de l’activite´sexuelle chez le bouc par des traitements photope´riodiques. Thesis, Montpellier, France, 119 s.
• Derashri, H.J., Pathak, A.K., Bansal, K.K., Sharma, A.K., Verma, S.K., 1992. Reproduction in buck: 2. Daily sperm output, extra-gonadal sperm reserve, daily sperm production rate and seminiferous tubule length. Pre-Conference Proceeding, Abstract of Contributory papers, 5th Int. Conf. on Goats, New Delhi vol. 1 s. 264.
• Gillan, L., Kroetsch, T., Chis Maxwell, W.M., Evans, G., 2008. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Animal Reproduction Science 103, 201–214.
• Graham, J.K., 2001. Assessment of sperm quality: a flow cytometric approach. Anim. Reprod. Sci. 68, 239–247.
• Graham, J.K., Mocé, E., 2005. Fertility evaluation of frozen/thawed semen. Theriogenology. 2005 Aug;64(3):492-504. doi: 10.1016/j.theriogenology.05.006. PMID: 15946734.
• Hafez, E.S.E., 1993. Reproduction in farm animals. 6. Edition. Lea&Febiger, Philadelphia.
• Herman, H.A., Mitchell, J.R., Doak, G.A., 1994. The Artificial Insemination and Embryo Transfer of Dairy and Beef Cattle. 7. Edition. Interstate Publishers, Inc.
• Holt, W., Watson, P., Curry, M., Holt, C., 1994. Reproducibility of computer-aided semen analysis: Comparison of five different systems used in a practical workshop. Fertil Steril 62, 1277-1282.
• Holt, W.V., 2000. Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology 53:47–58.
• Januskauskas, A., Johannisson, A., Soderquist, L., Rodriguez-Martinez, H., 2000. Assessment of sperm characteristics post-thaw and response to calcium ionophore in relation to fertility in Swedish dairy AI bulls. Theriogenology 53, 859–875.
• Karatzas, G., Karagiannidis, A., Varsakeli., S., Brikas, P., 1997. Fertility of fresh and frozen-thawed goat semen during the nonbreeding season. Hheriogenelogy 53, 47-58.
• Konyali, C., Tomás, C., Blanch, E., Gómez, E.A., Graham, J.K., Mocé, E., 2013. Optimizing conditions for treating goat semen with cholesterol-loaded cyclodextrins prior to freezing to improve cryosurvival. Cryobiology. 2013 Oct;67(2):124-31. doi: 10.1016/j.cryobiol.2013.06.001. Epub 2013 Jun 11. PMID: 23770515.001.
• Leboeuf, B., Restall, B., Salamon, S., 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science 62, 113-141.
• Mocé, E, Graham, J.K., 2008. In vitro evaluation of sperm quality. Anim Reprod Sci. 2008 Apr;105(1-2):104-18. doi: 10.1016/j.anireprosci.2007.11.016. Epub 2007 Nov 26. PMID: 18178345.
• Mocé, E., 2008. Notas de Biotecnologia de la Reproduccion. Valencia.
• Moore, A., I., Squires, E., L., Graham, J., K., 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology 51, 241–249.
• Parks, J. E., Lynch, D., V., 1992. Lipid composition and thermotropic phase behaviour of boar, bull, stallion and rooster sperm membranes. Cryobiology 29:255–266.
• Purdy, P. H., Graham, J., K., 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology 48:36–45.
• Purdy, P., 2005. A review on goat sperm cryopreservation. Small Ruminant Research.
• Roca, J., Carrizosa, J., A., Campos, I., Lafuente, A., Vazquez, J., M., Martinez, E., 1997. Viability and fertility of unwashed Murciano-Granadina goat spermatozoa dilte in Tris-egg yolk extender and stored at 5 0C. Small Ruminant Research 25, 147-153.
• Rodriguez-Martinez, H., 2006. Can we increase the estimate value of semen assessment? Reprod. Domest. Anim. 41 (Suppl. 2), 2–10.
• Rouger, Y., 1974. Etude des interactions de l’environnement et des hormones sexuelles dans la re´gulation du comportement sexuel des Bovidae. The`se de Doctorat, Universite´de Rennes, 197 s.
• Salamon, S., Ritar, A.J., 1982. Deep freezing of Angora goat semen: effects of diluent composition and method and rate of dilution on survival of spermatozoa. Aust. J. Biol. Sci. 35, 295–303.
• Salvador, I., Viudes-de-Castro M., P., Bernacer, J., Gomez, E., A., Silvestre, M., A., 2005. Factors affecting pregnancy rate in artificial insemination with frozen semen during non-breeding season in Murciano-Granadina goats. A field assay. Reproduction in domestic animals 40, 526-529.
• Torres, P., Serres, C., G´omez-Cu´etara, C., Santiago, I., Mateos, E., A´ lvarez, A., L., 2006. Effect of cholesterol-loaded cyclodextrin on motility and plasma membrane integrity of cooled stallion sperm. Animal Reproduction Science 94, 148–151
• Tuli, R.K., Holtz, W., 1992. The effect of season on seminal characters in Boer goat bucks in the Northern temperate zone. Recent Advances in Goat Production. Proc. papers presented at 5th Int. Conf. on Goats. New Delhi, Abstract No. 1195.
• Verstegen, J., Iguer-Ouada, M., Onclin K., 2002. Computer assisted semen analyzers in andrology research and veterınary practice. Theriogenology 57:149-l 79,2002. Elsevier Science Inc.
• Visconti, P. E., H. Galantino-Homer, X. Ning, G. D. Moore, J. P. Valenzuela, C. J. Jorgez, J. G. Alvarez, G. S. Kopf. 1999. Cholesterol efflux-mediated signal transduction in mammalian sperm. beta-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation. J. Biol. Chem. 274:3235–3242.
• Walkden-Brown, S.W., Restall, B.J., Norton, B.W., Scaramuzzi, R.J., 1994. The ‘female effect’ in Australian cashmere goats: effect of season and quality of diet on the LH and testosterone response of bucks to oestrous does. J. Reprod. Fertil. 100, 521–531.
• Watson, P. F. 1981. The effects of cold shock on sperm cell membranes. S. 189–218 in Effects of Low Temperatures on Biological Membranes. G. J. Morris and A. Clarke, ed. Academic Press, London, UK.
• White, I., G., 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reprod. Fertil. Dev. 5:639–658.