Research Article
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Alerjik dermatitli kedilerde ev tozu akarlarına spesifik in vitro Ig E düzeylerinin araştırılması

Year 2019, Volume: 4 Issue: 1, 14 - 17, 30.06.2019
https://doi.org/10.24880/maeuvfd.526315

Abstract

Alerji kavramı, neredeyse yüz yıl kadar önce bir çocuk
doktorunun, bağışıklık sistemi vakalarının reaktivitesini değiştiren bir
antijenle ilk bağlantısı olabileceği fikrini ortaya atmasıyla çıkmıştır. Son
yıllarda Veteriner hekimlikte bazı hipotezler ve yeni bulgular yapılmış, yine
de kedilerde ev tozu akarlarına bağlı oluşan alerjik dermatit ilgi çekmektedir.
Bu retrospektif araştırmanın amacı, alerjik dermatitli kedilerin
klinik bulgularının altında yatan nedenleri tespit etmektir. Dermatophagoides farina ve Dermatophagoides pteronyssinus’a karşı
alerjen spesifik Ig E konsantrasyonları, in vitro Polycheck Veteriner alerji
testleri kullanılarak belirlenmesiydi (RDA grup, İstanbul). Adnan Menderes Üniversitesi Veteriner Fakültesi İç
Hastalıkları Anabilim Dalı kliniğine primer / sekonder deri lezyonları olan
veya genel sağlık durumu kontrolü için gelen toplamda 33 kedi çalışmaya alındı.
Öncelikle mevcut hematolojik, mikrobiyolojik, parazitolojik ve ilgili analizler
yapıldı. On dokuz kedi, ilgili analize dayalı olarak sağlıklı kabul edildi (ne
enfeksiyon ne de aşırı duyarlılık gelişmiş) kontrol grubu olarak kaydedildi.
Diğer
14 kedi alerjik vakalardan oluşuyordu. 20 farklı
alerjene karşı spesifik IgE analizi için tüm kedilerden toplam 0.2 ml kan
alındı; bu yorumlama sadece ev tozu akarlarını içerdi.
Alerjik kedilerde
alerjene spesifik IgE seviyeleri (kU / l) ile ilgili olarak, in vitro testler, D. farinae için [sınıf 3,4 (n = 1),
sınıf 2 (n = 7), sınıf 1 (n = 6), sınıf 0 (n= 0)] ve D. pteronyssinus için[sınıf 3,4 (n = 1), sınıf 2 (n = 5), sınıf 1
(n = 4) ve sınıf 0 (n = 4)] pozitif reaksiyonlar verdi. Bütün alerjik kedilerde
en az 1 antijen saptandı. Sağlıklı kontrol grubu ve alerjik kediler arasında ev
tozu akarlarına karşı spesifik Ig E seviyeleri için istatistiksel olarak
anlamlı bir farklılık mevcuttu; D.
farinae
için 0.21 ± 0.018'e karşılık 4.69 ± 1.49 (p = 0.0001) ve D. pteronyssinus için 0.21 ± 0.023’e
karşılık 3.11 ± 1.21 (p = 0.003). Bu retrospektif çalışmada, Polycheck alerji
testindeki tüm pozitif reaksiyonların, kayıtlı kedilerde dermatitin ev tozu
alerjenlerini gösterdiği öne sürülmüştür. İn vitro alerji testlerinin yalnızca
benzersiz olmayan klinik bulgular nedeniyle sağlıklı vakalardan aşırı
duyarlılığı yansıtmayabileceği bilinen bir faktördür.

References

  • Referans1. Miller WH, Griffin CE, Campbell KL. In: Muller & Kirk’s Small Animal Dermatology. 7th ed. pp. 388-392. St. Louis, Missouri: Elsevier; 2013.Referans2. Hnilca KA. In:Small Animal Dermatology: A Color Atlas and Therapeutic Guide. 3rd ed. pp. 198-199. Elsevier Saunders; 2011. Referans3. O’Dair H, Markwell PJ, Maskell IE. An open investigation into the etiology in a group of cats with suspected allergic skin disease. Veterinary Dermatology 1996; 7: 193–202. DOİ: 10.1111/j.1365-3164.1996.tb00246.xReferans4. Gilbert S, Halliwell RE. Feline immunoglobulin E: Induction of antigen-specific antibody in normal cats and levels in spontaneously allergic cats. Veterinary Immunology and Immunopathology 1998; 63: 235–252. DOİ: 10.1016/S0165-2427(98)00100-7Referans5. Foster AP, O’Dair HA, DeBoer DJ. Allergen-specific IgG antibodies in cats with allergic skin disease. Research in Veterinary Science 1997; 63: 239–243. DOİ: 10.1016/S0034-5288(97)90027-8 Referans6. Halliwell REW, Gilbert SM, Lian TM. Induced and spontaneous IgE antibodies to Dermatophagoides farina in dogs and cats: Evidence of functional heterogeneiety of IgE. Veterinary Dermatology 1998; 9: 179–184. DOİ: 10.1046/j.1365-3164.1998.00112.xReferans7. Ural K, Erdoğan H, GÜLTEKİN M. Allergen specific IgE determination by in vitro allergy test in head and facial feline dermatitis: A pilot study. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2018; 65(4):379-386.Referans8. Ural K. Köpeklerde paraziter dermatozlar. p. 58-84. Ankara: Uzerler Matbaacılık Sanayi Ltd. Şti. ; 2014.Referans9. Belova S, Wilhelm S, Linek M, Beco L, Fontaine J, Bergvall K, Favrot C. Factors affecting allergen-specific IgE serum levels in cats. Canadian Journal of Veterinary Research 2012; 76(1): 45-51.Referans10. Gilbert S, Halliwell REW. The effects of endoparasitism on the immune response to orally administred antigen in cats. Veterinary Immunology and Immunopathology 2005; 106: 113–120. DOİ: doi.org/10.1016/j.vetimm.2005.01.009Referans11. Scott DW, Miller WH, Griffin CE In: Small Animal Dermatology. 6th ed. W.B. Saunders Company: Philadelphia; 2001.Referans12. Moriello KA. Case report: Feline atopy in three littermates. Veterinary Dermatology 2001; 12: 177-181. DOİ: doi.org/10.1046/j.1365-3164.2001.00246.xReferans13. Guaguere E, Prelaud PA. In: Practical guide to feline dermatology. Merial: New York;1999.Referans14. Prost, C. Feline atopic dermatitis: clinical signs and diagnosis. European Journal of Companion Animal Practice 2009; 19(3): 223-229.Referans15. Locke PH, Harvey RG, Mason IS. In: Manual of small animal dermatology. British Small Animal Veterinary Association (BSAVA); 1993.Referans16. Moriello KA, Masom I. In: Handbook of small animal dermatology. Elsevier Science Ltd: Oxford; 1995. Referans17. Szczepanik M, Pomorska D, Wilkołek P. Diagnostic approach to atopy in cats. Bulletin of the Veterinary Institute in Pulawy 2016; 52: 477-480. Referans18. Bexley J, Hogg JE, Hammerberg B, Halliwell RE. Levels of house dust mite‐specific serum immunoglobulin E (IgE) in different cat populations using a monoclonal based anti‐IgE enzyme‐linked immunosorbent assay. Veterinary dermatology 2009; 20(5‐6): 562-568. DOİ: 10.1111/j.1365-3164.2009.00840.xReferans19. Loft KE, Rosser Jr EJ. (2010). Group 1 and 2 Dermatophagoides house dust mite allergens in the microenvironment of cats. Veterinary dermatology 2010; 21(2): 152-158. DOİ: 10.1111/j.1365-3164.2009.00771.x

House dust mite specific in vitro IgE determination in cats with allergic dermatitis

Year 2019, Volume: 4 Issue: 1, 14 - 17, 30.06.2019
https://doi.org/10.24880/maeuvfd.526315

Abstract

Allergy, nearly a hundred years ago, a
paediatrician comprehended the idea of allergy, the initial connection of the
immune system via an antigen altering the reactivity of the cases. In recent
years several hypothesis and novel findings have been conducted in veterinary
medicine, yet allergic dermatitis due to house dust mites ar of interest in
feline medicine. The purpose of this retrospective reserach was to verify
underlying causes of clinical findings to those of cats with allergic
dermatitis. Allergen speicific Ig E concentrations against Dermatophagoides farinae and Dermatophagoides
pteronyssinus
were determined by use of in vitro Polycheck Veterinary
allergy tests (RDA grup, İstanbul). In a total of 33 cats were introduced to
the Adnan Menderes University, Faculty of Veterinary, Department of Internal
Medicine clinic with primary/secondary skin lesions or to those of general
health status check up. On initial referaal available haematological,
microbiological, parasitological and relevant tests were performed. Nineteen
cats were deemed healthy based on relevant analysis (neither infection nor
hypersensitivity) were enrolled as a control group. Other 14 cats were composed
of allergic cases. A total 0.2 ml of blood was taken from all cats for specific
IgE analysis against 20 different allergens, in which interpretation involved
solely house dust mites. Regarding allergen specific IgE levels (kU/l) in
allergic cats, in vitro tests gave a positive reactions for D. farinae [class 3,4 (n=1), class 2
(n=7), class 1 (n=6) and class 0 (n=0)] and D.
pteronyssinus
[class 3,4 (n=1), class 2(n=5), class 1(n=4) and class 0
(n=4)] in whom at least 1 antigen was detected in all allergic cats. There was a statistical significance for
specific Ig E leves between healthy controls and allergic cats against house
dust mites as follows; 0.21 ± 0.018 vs. 4.69 ± 1.49 for D. farinae (p=0.0001) and 0.21 ± 0.023 vs. 3.11 ± 1.21 (p=0.003)
for D. pteronyssinus. In the
present retrospective study, all positive reactions on the Polycheck allergy
test were suggested to present underlying house dust allergens of dermatitis in
enrolled cats. As far as well known factor that in vitro allergy tests solely
might not be reflecting hypersensitivity from healthy cases because of
nonunique clinically findings 

References

  • Referans1. Miller WH, Griffin CE, Campbell KL. In: Muller & Kirk’s Small Animal Dermatology. 7th ed. pp. 388-392. St. Louis, Missouri: Elsevier; 2013.Referans2. Hnilca KA. In:Small Animal Dermatology: A Color Atlas and Therapeutic Guide. 3rd ed. pp. 198-199. Elsevier Saunders; 2011. Referans3. O’Dair H, Markwell PJ, Maskell IE. An open investigation into the etiology in a group of cats with suspected allergic skin disease. Veterinary Dermatology 1996; 7: 193–202. DOİ: 10.1111/j.1365-3164.1996.tb00246.xReferans4. Gilbert S, Halliwell RE. Feline immunoglobulin E: Induction of antigen-specific antibody in normal cats and levels in spontaneously allergic cats. Veterinary Immunology and Immunopathology 1998; 63: 235–252. DOİ: 10.1016/S0165-2427(98)00100-7Referans5. Foster AP, O’Dair HA, DeBoer DJ. Allergen-specific IgG antibodies in cats with allergic skin disease. Research in Veterinary Science 1997; 63: 239–243. DOİ: 10.1016/S0034-5288(97)90027-8 Referans6. Halliwell REW, Gilbert SM, Lian TM. Induced and spontaneous IgE antibodies to Dermatophagoides farina in dogs and cats: Evidence of functional heterogeneiety of IgE. Veterinary Dermatology 1998; 9: 179–184. DOİ: 10.1046/j.1365-3164.1998.00112.xReferans7. Ural K, Erdoğan H, GÜLTEKİN M. Allergen specific IgE determination by in vitro allergy test in head and facial feline dermatitis: A pilot study. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2018; 65(4):379-386.Referans8. Ural K. Köpeklerde paraziter dermatozlar. p. 58-84. Ankara: Uzerler Matbaacılık Sanayi Ltd. Şti. ; 2014.Referans9. Belova S, Wilhelm S, Linek M, Beco L, Fontaine J, Bergvall K, Favrot C. Factors affecting allergen-specific IgE serum levels in cats. Canadian Journal of Veterinary Research 2012; 76(1): 45-51.Referans10. Gilbert S, Halliwell REW. The effects of endoparasitism on the immune response to orally administred antigen in cats. Veterinary Immunology and Immunopathology 2005; 106: 113–120. DOİ: doi.org/10.1016/j.vetimm.2005.01.009Referans11. Scott DW, Miller WH, Griffin CE In: Small Animal Dermatology. 6th ed. W.B. Saunders Company: Philadelphia; 2001.Referans12. Moriello KA. Case report: Feline atopy in three littermates. Veterinary Dermatology 2001; 12: 177-181. DOİ: doi.org/10.1046/j.1365-3164.2001.00246.xReferans13. Guaguere E, Prelaud PA. In: Practical guide to feline dermatology. Merial: New York;1999.Referans14. Prost, C. Feline atopic dermatitis: clinical signs and diagnosis. European Journal of Companion Animal Practice 2009; 19(3): 223-229.Referans15. Locke PH, Harvey RG, Mason IS. In: Manual of small animal dermatology. British Small Animal Veterinary Association (BSAVA); 1993.Referans16. Moriello KA, Masom I. In: Handbook of small animal dermatology. Elsevier Science Ltd: Oxford; 1995. Referans17. Szczepanik M, Pomorska D, Wilkołek P. Diagnostic approach to atopy in cats. Bulletin of the Veterinary Institute in Pulawy 2016; 52: 477-480. Referans18. Bexley J, Hogg JE, Hammerberg B, Halliwell RE. Levels of house dust mite‐specific serum immunoglobulin E (IgE) in different cat populations using a monoclonal based anti‐IgE enzyme‐linked immunosorbent assay. Veterinary dermatology 2009; 20(5‐6): 562-568. DOİ: 10.1111/j.1365-3164.2009.00840.xReferans19. Loft KE, Rosser Jr EJ. (2010). Group 1 and 2 Dermatophagoides house dust mite allergens in the microenvironment of cats. Veterinary dermatology 2010; 21(2): 152-158. DOİ: 10.1111/j.1365-3164.2009.00771.x
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Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Articles
Authors

Kerem Ural 0000-0003-1867-7143

Serdar Paşa 0000-0003-4957-9263

Hasan Erdoğan 0000-0001-5141-5108

Mehmet Gültekin 0000-0002-5197-2403

Deniz Alıç Ural 0000-0002-2659-3495

Songül Erdoğan This is me 0000-0002-7833-5519

Halil İbrahim Adak This is me 0000-0002-6844-2169

Deniz Sude Ateş 0000-0001-7052-2697

Gizem Gül 0000-0002-5752-8696

Publication Date June 30, 2019
Submission Date February 12, 2019
Published in Issue Year 2019 Volume: 4 Issue: 1

Cite

APA Ural, K., Paşa, S., Erdoğan, H., Gültekin, M., et al. (2019). House dust mite specific in vitro IgE determination in cats with allergic dermatitis. Veterinary Journal of Mehmet Akif Ersoy University, 4(1), 14-17. https://doi.org/10.24880/maeuvfd.526315