Research Article
BibTex RIS Cite

Saanen keçilerinde çiftleşme sonrası progesteron ve gonadotropin uygulamaları ve gebelik oranlarının belirlenmesi

Year 2022, Volume: 7 Issue: 1, 1 - 6, 30.04.2022
https://doi.org/10.24880/maeuvfd.987935

Abstract

Keçilerde, üreme mevsimi dışında yapılan senkronizasyon uygulamaları sonrasında karşılaşılan embriyonik kayıpların %70’i çiftleşme sonrası görülen luteal yetersizlikler nedeniyle şekillenmektedir. Bu çalışmanın amacı; luteal yetersizlikler nedeniyle oluşan embriyonik kayıpların önlenmesi için; anöstrus sezonunda çiftleşme sonrasında yapılan GnRH ya da progesteron uygulamalarının gebelik oranları üzerine etkilerinin araştırılmasıdır. Çalışmada herhangi infertilite sorunu bulunmayan ve anöstrus sezonunda bulunan toplam 30 baş Saanen keçisi kullanılmıştır. Keçiler rastgele 3 gruba ayrılmıştır. Tüm gruplarda progesteron içeren intravaginal araç (CIDR®) kullanılarak senkronizasyon programı uygulanmış ve teke katılmıştır. G1’de (n=10) çiftleşmelerden sonra hiçbir uygulama yapılmamıştır. G2’de (n=10) keçilere son çiftleşmeden sonraki 12. gün buserelin enjeksiyonu yapılmıştır. G3’de (n=10) son çiftleşmeden sonraki 5. gün 5 gün süreyle CIDR® uygulanmıştır. Çiftleşme sonrası tüm keçilerden 12. ve 30. günlerde kan örneği alınmış ve 30. gün gebelik muayeneleri yapılmıştır. Çalışma sonunda, gruplar arasında gebelik oranları açısından bir fark oluşmadığı (p>0.05), her bir grupta alınan 12. ve 30. gün kan örneklerinde progesteron değerleri incelendiğinde; G2 hariç hiçbir grupta istatistiksel bir fark bulunmadığı belirlenmiştir. G2’de 30. gün ölçülen progesteron değerlerinin, G1 ve G3’de ölçülen değerlerden istatistiksel olarak farklı olduğu belirlenmiştir (p<0.01). Sonuç olarak çiftleşme sonrası yapılan buserelin veya intravaginal progesteron uygulamalarının gebelik oranları üzerine bir etkisinin olmadığı ancak; buserelin enjeksiyonlarının progesteron seviyelerini önemli derecede yükselttiği belirlenmiştir.

Supporting Institution

Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

Proje numarası: 0405YL16

References

  • Whitley NC, Jacson DJ. An update on estrus synchronization in goats: A minor species. J Anim Sci. 2004; 82: 270-276.
  • Alaçam E. Üremenin kontrolü. (Ed.): Alaçam E. Evcil Hayvanlarda Doğum ve İnfertilite (5.Baskı), Medisan, Ankara, 2005; s: 71-80.
  • İnce D, Köker A. The effect of estrus synchronization on the reproductive chracateristics of Turkish Saanen goats and growth characteristics of kids under extensive conditions. African J Agri Res. 2011; 6(26): 5715-5719.
  • Cinar M, Ceyhan A, Yilmaz O, Erdem H (2017): Effect of estrus synchronization protocols including PGF2a and GnRH on fertility parameters in hair goats during breeding season. J Anim Plant Sci. 2017; 27(4): 1083-1087.
  • Özyurtlu N, Ay SS, Küçükaslan İ, Güngör Ö, Aslan S. Effect of subsequent two short-term, short-term and long-term progestagen treatment on fertility of Awassi ewes out of the breeding season. Ankara Univ Vet Fak Derg. 2011; 58: 105-109.
  • Özyurtlu N, Bademkıran S. Koyunlarda ÖstrusSenkronizasyonu ve Östrusu Uyarma Yöntemleri. Dicle Üniv Vet Fak Derg. 2010; 1(1): 17-22.
  • Uslu BA, Gülyüz F. Erken Anöstrus Döneminde Renkli Tiftik Keçilerinde İntravaginal Sünger, CIDR-G ve Kulak İmplantı Uygulamalarını Takiben GnRH Enjeksiyonunun Fertilite Üzerine Etkisi. Kafkas Univ Vet Fak Derg. 2009; 15 (3): 385-390.
  • Baldassarre H, Karatzas CN. Advanced assisted reproduction Technologies (ART) in goats. Anim Reprod Sci. 2004; 82-83:255-266.
  • Özer MÖ, Doğruer G. Aşım sezonunda Şami keçilerinde progestagen içeren deri altı implant ve vaginal süngerlerin uzun ve kısa süreli uygulamalarının fertilite üzerine etkisi. Kafkas Üniv Vet Fak Derg. 2011; 17: 47-52.
  • Leboeuf B, Forgerit Y, Bernelas D, Pougnard JL, Senty E, Driancourt MA. Efficacy of two types of vaginal sponges to control onset of oestrus, time of preovulatory LH peak and kidding rate in goats inseminated with variable numbers of spermatozoa. Theriogenology, 2003; 60: 1371-1378.
  • Martemucci G, D’Alessandro AG. Induction/synchronization of oestrus and ovulation in dairy goats with different short term treatments and fixed time intrauterine or exocervical insemination system. Anim Reprod Sci. 2011; 126: 187194.
  • Garrett JE, Geisert RD, Zavy MT, Morgan GL. Evidence for maternal regulation of early conceptus growth and development in beef cattle. J Reprod Fertil. 1988; 84: 437-446. Kleemann DO, Walker SK, Seamark RF. Enhanced fetal growth in sheep administered progesterone during the Srst three days of pregnancy. J. Reprod Fertil. 1994; 102: 411-417.
  • Mcmillan KL, Day AM, Taufa VK, Gibb M, Pearce MG. Effects of an agonist of gonadotrophin releasing hormone in cattle 1. Hormone concentrations and cycle length. Anim Reprod Sci. 1985; 8: 203-212. Nephew KP, Cardenas H, McClure KE, Ott TL, Bazer FW, Pope WF. Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J Anim Sci. 1994; 72: 453-458.
  • Fonseca JF, Silva Filho JM, Pinto Neto A, Palhares MS, Ruas JRM, Alvin MTT, BelissaÅL rio H, Saliba WP. Plasma progesterone concentration in recipient heifers submitted to administration of rbST, GnRH or hCG on day five of the estrous cycle Arq Bras Med Vet Zootec. 2001; 53(4): 451– 458.
  • Khan TH, Beck NFG, Khalid M. The effects of GnRH analouge (buserelin) or hCG (chorulon) on day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. Anim Reprod Sci. 2007; 102(3-4): 247-257.
  • Nava-Trujillo H, Chango-Villasmil J, Finol-Parra G, Maldonado-Suarez J, Torres-Rodriguez P, Carrillo-Fernandez F, Gil-Huerta L, Gonzales N. Brief communication: effect of post-mating progestagen administration on pregnancy rate in crossbreed goats following an induced estrus. Revista Cientifica. 2008; 18(5): 578-581.
  • McCracken JA, Custer EE, Lamsa JC. Luteolysis; A Neuroendocrine Med Lated Event. Physiol Rev. 1999; 79: 263-324.
  • Farin CE, Moeller CL, Mayan H, Gamboni F, Sawyer HR, Niswender GD. Effect of luteinizing hormone and human chorionic gonadotropin on cell populations in the ovine corpus luteum. Biol Reprod. 1988; 38: 413–421.
  • Fitz TA, Mayan MH, Sawyer HR, Niswender GD. Characterisation of two steroidogenic cell types in the ovine corpus luteum. Biol Reprod. 1982; 27: 703-711.

Administration of progesterone and gonatotropin after breeding and determination of pregnancy rates in Saanen goats

Year 2022, Volume: 7 Issue: 1, 1 - 6, 30.04.2022
https://doi.org/10.24880/maeuvfd.987935

Abstract

Seventy percent of the embryonic losses after synchronization out of breeding season in goats are originated due to luteal insufficiency. The aim of this study was to investigate the effects of administrations of GnRH or progesterone after mating on pregnancy rates during anestrus season. A total of 30 heads of Saanen goats were used during the anestrus season, with no infertility problems. The goats are randomly divided into 3 groups. Synchronization program was applied by using intravaginal device containing progesterone (CIDR®) in all groups and mated in bucks. In G1 (n = 10) no application was performed after mating. In G2 (n=10) buserelin injections were made on the 12th day after the last mating. In G3 (n = 10), CIDR® was applied for 5 days on 5th day after last mating. Blood samples were taken at 12th and 30th days after mating from all goats and 30th day pregnancy examinations were performed. There was no difference between the groups in terms of pregnancy rates (p> 0.05), when the progesterone values were examined in blood samples taken at 12th and 30th days in each group; no statistically significant difference was found in any group except G2. The progesterone values measured in G2 on the 30th day were statistically different from the values measured in G1 and G3 (p <0.01). In conclusion, post mating buserelin or intravaginal progesterone administration has no effect on pregnancy rates; buserelin injections have significantly increased progesterone levels.

Project Number

Proje numarası: 0405YL16

References

  • Whitley NC, Jacson DJ. An update on estrus synchronization in goats: A minor species. J Anim Sci. 2004; 82: 270-276.
  • Alaçam E. Üremenin kontrolü. (Ed.): Alaçam E. Evcil Hayvanlarda Doğum ve İnfertilite (5.Baskı), Medisan, Ankara, 2005; s: 71-80.
  • İnce D, Köker A. The effect of estrus synchronization on the reproductive chracateristics of Turkish Saanen goats and growth characteristics of kids under extensive conditions. African J Agri Res. 2011; 6(26): 5715-5719.
  • Cinar M, Ceyhan A, Yilmaz O, Erdem H (2017): Effect of estrus synchronization protocols including PGF2a and GnRH on fertility parameters in hair goats during breeding season. J Anim Plant Sci. 2017; 27(4): 1083-1087.
  • Özyurtlu N, Ay SS, Küçükaslan İ, Güngör Ö, Aslan S. Effect of subsequent two short-term, short-term and long-term progestagen treatment on fertility of Awassi ewes out of the breeding season. Ankara Univ Vet Fak Derg. 2011; 58: 105-109.
  • Özyurtlu N, Bademkıran S. Koyunlarda ÖstrusSenkronizasyonu ve Östrusu Uyarma Yöntemleri. Dicle Üniv Vet Fak Derg. 2010; 1(1): 17-22.
  • Uslu BA, Gülyüz F. Erken Anöstrus Döneminde Renkli Tiftik Keçilerinde İntravaginal Sünger, CIDR-G ve Kulak İmplantı Uygulamalarını Takiben GnRH Enjeksiyonunun Fertilite Üzerine Etkisi. Kafkas Univ Vet Fak Derg. 2009; 15 (3): 385-390.
  • Baldassarre H, Karatzas CN. Advanced assisted reproduction Technologies (ART) in goats. Anim Reprod Sci. 2004; 82-83:255-266.
  • Özer MÖ, Doğruer G. Aşım sezonunda Şami keçilerinde progestagen içeren deri altı implant ve vaginal süngerlerin uzun ve kısa süreli uygulamalarının fertilite üzerine etkisi. Kafkas Üniv Vet Fak Derg. 2011; 17: 47-52.
  • Leboeuf B, Forgerit Y, Bernelas D, Pougnard JL, Senty E, Driancourt MA. Efficacy of two types of vaginal sponges to control onset of oestrus, time of preovulatory LH peak and kidding rate in goats inseminated with variable numbers of spermatozoa. Theriogenology, 2003; 60: 1371-1378.
  • Martemucci G, D’Alessandro AG. Induction/synchronization of oestrus and ovulation in dairy goats with different short term treatments and fixed time intrauterine or exocervical insemination system. Anim Reprod Sci. 2011; 126: 187194.
  • Garrett JE, Geisert RD, Zavy MT, Morgan GL. Evidence for maternal regulation of early conceptus growth and development in beef cattle. J Reprod Fertil. 1988; 84: 437-446. Kleemann DO, Walker SK, Seamark RF. Enhanced fetal growth in sheep administered progesterone during the Srst three days of pregnancy. J. Reprod Fertil. 1994; 102: 411-417.
  • Mcmillan KL, Day AM, Taufa VK, Gibb M, Pearce MG. Effects of an agonist of gonadotrophin releasing hormone in cattle 1. Hormone concentrations and cycle length. Anim Reprod Sci. 1985; 8: 203-212. Nephew KP, Cardenas H, McClure KE, Ott TL, Bazer FW, Pope WF. Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J Anim Sci. 1994; 72: 453-458.
  • Fonseca JF, Silva Filho JM, Pinto Neto A, Palhares MS, Ruas JRM, Alvin MTT, BelissaÅL rio H, Saliba WP. Plasma progesterone concentration in recipient heifers submitted to administration of rbST, GnRH or hCG on day five of the estrous cycle Arq Bras Med Vet Zootec. 2001; 53(4): 451– 458.
  • Khan TH, Beck NFG, Khalid M. The effects of GnRH analouge (buserelin) or hCG (chorulon) on day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. Anim Reprod Sci. 2007; 102(3-4): 247-257.
  • Nava-Trujillo H, Chango-Villasmil J, Finol-Parra G, Maldonado-Suarez J, Torres-Rodriguez P, Carrillo-Fernandez F, Gil-Huerta L, Gonzales N. Brief communication: effect of post-mating progestagen administration on pregnancy rate in crossbreed goats following an induced estrus. Revista Cientifica. 2008; 18(5): 578-581.
  • McCracken JA, Custer EE, Lamsa JC. Luteolysis; A Neuroendocrine Med Lated Event. Physiol Rev. 1999; 79: 263-324.
  • Farin CE, Moeller CL, Mayan H, Gamboni F, Sawyer HR, Niswender GD. Effect of luteinizing hormone and human chorionic gonadotropin on cell populations in the ovine corpus luteum. Biol Reprod. 1988; 38: 413–421.
  • Fitz TA, Mayan MH, Sawyer HR, Niswender GD. Characterisation of two steroidogenic cell types in the ovine corpus luteum. Biol Reprod. 1982; 27: 703-711.
There are 19 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research Articles
Authors

Mahmut İbiş 0000-0003-3350-3184

Ali Reha Ağaoğlu 0000-0002-6545-8800

Project Number Proje numarası: 0405YL16
Publication Date April 30, 2022
Submission Date August 27, 2021
Published in Issue Year 2022 Volume: 7 Issue: 1

Cite

APA İbiş, M., & Ağaoğlu, A. R. (2022). Saanen keçilerinde çiftleşme sonrası progesteron ve gonadotropin uygulamaları ve gebelik oranlarının belirlenmesi. Veterinary Journal of Mehmet Akif Ersoy University, 7(1), 1-6. https://doi.org/10.24880/maeuvfd.987935