An optimized protocol for the electroporation of NCI H929 multiple myeloma cells

Year 2022, Volume: 7 Issue: 3, 188 - 194, 31.12.2022

Ayşe Kızılyer

https://doi.org/10.24880/maeuvfd.1126466

Abstract

Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods. While electroporation is the most efficient tool for the transfection of most hard-to-transfect cells, human multiple myeloma cells differ in their permissiveness and each cell type require different electroporation conditions for an efficient transgene delivery. In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol. Findings from this paper showed that besides the voltage and capacitance settings, cell count, the cell cycle status of cells, the amount of nucleic acid and removal of death cells all impacted the electroporation efficiency and viable cell count. These results are expected to serve as a starting point and a guide for researchers.

Keywords

electroporation, multiple myeloma, NCI H929, transfection

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