Efficacy of Chitosan and Some Essential Oil Combinations in Tomato on Root knot Nematode

Yıl 2023, , 108 - 114, 22.06.2023

Fatma Gül Göze Özdemir , Hacer Çevik Jean Claude Ndayıragıje , Tuğçe Özek , İsmail Karaca

https://doi.org/10.29048/makufebed.1246991

Öz

In this study, the effects of single and combination applications of chitosan and commercial thyme, sesame, garlic, fennel, grapefruit, pomegranate and mustard essential oils to the soil on Meloidogyne incognita in tomato roots were investigated. The study consisted of 17 applications, each application was set up in a randomized plot design with 5 replications. A thousand M. incognita second-stage larva/1ml was used for each seedling and applications were made one day later. While the essential oil was applied to the soil at 1000 ppm for each pot, 5 ml of the 1% liquid suspension prepared from chitosan was used. The study was terminated after 60 days and evaluation was made according to the 1-9 gal and egg masses index. Root nematode reproduction was found to be lower in chitosan and all essential oil applications compared to the negative control. When essential oils to the soil together with chitosan is compared with their single application, the gall and egg mass index values in tomato roots decreased by 50%. Combination of chitosan with essential oil of thyme, pomegranate and mustard had a similar effect with nematicide. These results show that essential oils are good alternatives to increase the nematicidal effect of chitosan.

Anahtar Kelimeler

Mustard, thyme, chitosan, Meloidogyne incognita, essential oil

Destekleyen Kurum

X

Proje Numarası

X

Teşekkür

X

Domateste Kitosan ve Bazı Uçucu Yağ Kombinasyonlarının Kök Ur Nematodu Üzerindeki Etkinliği

Öz

Bu çalışmada domateste toprağa kitosan ile ticari kekik, susam, sarımsak, rezene, greyfurt, nar ve hardal esansiyel yağlarının tek başına ve kombinasyon uygulamalarının köklerde Meloidogyne incognita gelişimi üzerine etkileri araştırılmıştır. Çalışma 17 uygulamadan oluşmuş, her uygulama 5 tekerrürlü tesadüf parselleri deneme deseninde kurulmuştur. Nematod inokulasyonlarında her bir fide için 1000 M. incognita ikinci dönem larva/1ml kullanılmış ve bir gün sonra uygulamalar yapılmıştır. Uygulamaya göre her saksı için toprağa uçucu yağ 1000 ppm dozunda uygulanırken, kitosan hazırlanan %1‘lik sıvı süspansiyonundan 5 ml kullanılmıştır. Çalışma 60 gün sonra sonlandırılmış ve değerlendirme 1-9 gal ve yumurta paketi indeksine göre yapılmıştır. Köklerde nematod gelişimi, kitosan ve tüm uçucu yağ uygulamalarında negatif kontrole göre daha düşük bulunmuştur. Uçucu yağların kitosanla birlikte toprağa uygulanması tekli uygulamaları ile karşılaştırıldığında domates köklerinde gal ve yumurta paketi indeks değerleri %50 oranında azalmıştır. Kitosanın kekik, nar ve hardal uçucu yağ ile kombinasyonu nematisit ile benzer etkide bulunmuştur. Bu sonuçlar kitosanın nematisidal etkisinin artırılmasında esansiyel yağların iyi birer alternatif olduğunu göstermektedir.

Anahtar Kelimeler

Hardal, kekik, kitosan, Meloidogyne incognita, uçucu yağ

Proje Numarası

X

Kaynakça

• Abolusoro, S. A., Oyedunmade, E. A., Olabiyi, T.I. (2010). Evaluation of sweet orange peel aqueous extract (Cit-rus sinensis) as root–knot nematode suppressant. AgroScience, 9(3):170-175.
• Ashraf, M.S., Khan, T.A. (2010). Integrated approach for the management of Meloidogyne javanica on eggplant using oil cakes and biocontrol agents. Archives of Phytopathology and Plant Protection, 43(6): 609-614.
• Asif, M., Ahmad, F., Tariq, M., Khan, A., Ansari, T., Khan, F., Siddiqui, A.M. (2017). Potential of chitosan alone and in combination with agricultural wastes against the root-knot nematode, Meloidogyne incognita infesting eggplant. Journal of Plant Protection Research, 57(3): 288-295.
• Benit, N., Kumar, T.S.J., Almaary, K.S., Elshikh, M.S., Ras-heed, R.A., Antonisamy, P. (2022). Phytopathogenic bacterial and nematicidal activity of extracts and powder of Adhatoda vasica on Meloidogyne incognita. Physiological and Molecular Plant Pathology, 119: 101816; DOI: 10.1016/j.pmpp.2022.101816
• Berini, F., Katz, C., Gruzdev, N., Casartelli, M., Tettamanti, G., Marinelli, F. (2018). Microbial and viral chitinases: Attractive biopesticides for integrated pest management. Biotechnology Advances, 36(3): 818–838.
• Cetintas, R., Yarba, M.M. (2010). Nematicidal effect of five plant essential oils on the Root-knot nematode, Meloi-dogne incognita race 2. Journal of Animal and Veterinary Advances, 9(2): 222-225.
• Chen, J. X., Song, B. A. (2021). Natural nematicidal active compounds: Recent research progress and outlook. Journal of Integrative Agriculture, 20(8): 2015-2031.
• D’Addabbo, T., Tava, A., Argentieri, M.P., Biazzi, E., Can-dido, V., Avato, P. (2022). Nematicidal Potential of Sul-la (Hedysarum coronarium, L.) against the root-knot nematode Meloidogyne incognita. Plants, 11: 2550; DOI: 10.3390/plants11192550
• Deng, X., Wang, X., Li, G. (2022). Nematicidal effects of volatile organic compounds from microorganisms and plants on plant-parasitic nematodes. Microorganisms, 10(6): 1201; DOI: 10.3390/microorganisms10061201
• Echeverrigaray, S., Zacaria, J., Beltrão, R. (2010). Nematicidal activity of monoterpenoids against the Root-knot nematode Meloidogyne incognita. Phytopathology, 100(2): 199-203.
• El-Gindi, A. Y., Hamida, A. O., Youseff, M. M., Ameen, H. A., Asmahan, M. L. (2005). Evaluation of the nematicidal effects of aqueous and volatile oil extracts of some plants on the Root-knot nematode Meloidogyne incognita. Pakistan Journal of Nematology, 23(2): 233-239.
• El-Sayed, S.M., Mahdy, M.E. (2015). Effect of chitosan on root-knot nematode, Meloidogyne javanica on tomato plants. International Journal of ChemTech Research, 7(4): 1985-1992.
• Emam, A.M., Ahmed, M.A.M., Tammam, M.A.A., Hala, A.M., El-Dakar, Z.S. (2015). Isolation and structural identification of compounds with antioxidant, nemati-cidal and fungicidal activities from Punica granatum L. var. nana. International Journal of Science Enginering Research, 6: 1023–1040.
• Fan, Z., Wang, L., Qin, Y., Li, P. (2023). Activity of chi-tin/chitosan/chitosan oligosaccharide against plant pathogenic nematodes and potential modes of application in agriculture: A review. Carbohydrate Polymers, 120592; DOI: 10.1016/j.carbpol.2023.120592
• Figueiredo, A.C., Barroso, J.G., Pedro, L.G., Scheffer J.J.C. (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23(4):213-226.
• Forghani, F., Hajihassani, A. (2020).Recent advances in the development of environmentally benign treatments to control root-knot nematodes. Frontiers in Plant Science, 11: 1125; DOI: 10.3389/fpls.2020.01125
• Ghasemi, H., Fatemy, S. (2014). In vitro examination of mustard and tarragon extracts on the activity of Globodera rostochiensis. Aspects of Applied Biology, 126: 139-143.
• Göze Özdemir, F.G., Tosun, B., Şanlı, A., Karadoğan, T. (2022a). Bazı Apiaceae uçucu yağlarının Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae)'ya karşı nematoksik etkisi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 59(3): 529-539.
• Göze Özdemir, F.G., Çevik, H., Ndayıragıje, J. C., Özek, T., Karaca, İ. (2022b). Nematicidal effect of chitosan on Meloidogyne incognita in vitro and on tomato in a pot experiment. International Journal of Agriculture Environment and Food Sciences, 6(3): 410-416.
• Göze Özdemir, F.G. (2022a). Management of disease complex of Meloidogyne incognita and Fusarium oxysporum f. sp. radicis lycopersici on tomato using some essential oils. Plant Protection Bulletin, 62(4): 27-36.
• Göze Özdemir, F.G. (2022b). Limon, portakal, greyfurt ve nar meyvelerinin kabuklarının Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949'ya karşı kullanımı. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13(2): 221-228.
• Göze, F.G., Kara, A., Söğüt, M.A., Uysal, G. (2014). Bazı bitkisel yağların Meloidogyne incognita'ya karşı nematisidal etkinliklerinin belirlenmesi. 22. Ulusal Biyoloji Kongresi, 23-27 Haziran, 2014, Eskişehir,505.
• Henderson, D. R., Riga, E., Ramirez, R. A., Wilson, J., Snyder, W. E. (2009). Mustard biofumigation disrupts biological control by Steinernema spp. Nematodes in the soil. Biological control, 48(3): 316-322.
• Holland, D., Hatib, K., Bar-Ya’akov, I. (2009). Pomegrana-te: Botany, horticulture, breeding. In: Horticultural reviews. Janick, J. and Hoboken, N.J. (eds.), New York, USA, 127–191.
• İmamoğlu, Ö. (2011). Biyokontrolde doğal ürünlerin kullanılması; Kitosan. Türk Hijyen ve Deneysel Biyoloji, 68(4): 215-222.
• Kago, E. K., Kinyua, Z. M., Okemo, P. O., Maingi, J. M. (2013). Efficacy of brassica tissue and chalim TM on control of plant parasitic nematodes. Journal of Bio-logy, 1(1): 25-32.
• Kalaiarasan, P., Lakshmanan, P., Rajendran, G., Sami-yappan, R. (2006). Chitin and chitinolytic biocontrol agents for the management of root knot nematode, Meloidogyne arenaria in groundnut (Arachis hypogaea L.) cv. Co3. Indian Journal of Nematology, 36(2): 181–186.
• Kiewnick, S., Sikora, R. A. (2006). Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biological control, 38(2): 179-187.
• Kim, J.I., Han, S.C. (1988). Effect of solarization for control of root-knot nematode (Meloidogyne spp.) in vinyl house. Korean Journal of Applied Entomology, 27: 1-5.
• Ladner, D.C., Tchounwou, P.B., Lawrence, G.W. (2008). Evaluation of the effect of Ecologic on root knot nematode, Meloidogyne incognita, and tomato plant, Lycopersicon esculentum. International Journal of Environment Research and Public Health, 5(2): 104–110.
• Liu, Z., Li, Q. X., Song, B. (2022). Pesticidal activity and mode of action of monoterpenes. Journal of Agricultural and Food Chemistry, 70(15): 4556-4571
• Matthiessen, J.N., Kirkegaard, J.A. (2006). Biofumigation and enhanced biodegradation: Opportunity and challenge in soilborne pest and disease management. Critical reviews in plant sciences, 25(3): 235-265.
• Meyer, S.I., K.R. Chauhan, MacDonald, M.H. (2016). Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita. Nematropica, 46: 85-96.
• Mittal, N., Saxena, G., Mukerji, K. G. (1995). Integrated control of root-knot disease in three crop plants using chitin and Paecilomyces lilacinus. Crop Protection, 14(8): 647–651.
• Mota, L. C. B. M., dos Santos, M. A. (2016). Chitin and chitosan on Meloidogyne javanica management and on chitinase activity in tomato plants. Tropical Plant Pathology, 41(2): 84–90.
• Mouniga, R., Anita, B., Shanthi, A., Lakshmanan, A., Kart-hikeyan, G. (2022). Phenol and antioxidant enzymatic activity in root knot nematode, Meloidogyne incognita infected tomato plants treated with chitosan nanoparticles. The Pharma Innovation Journal, 11(4): 241-245
• Mukhtar Ahmed, K. B., Khan, M. M. A., Siddiqui, H., Jahan, A. (2020). Chitosan and its oligosaccharides, a promi-sing option for sustainable crop production - A review. Carbohydrate Polymers, 227: 115331; DOI: 10.1016/j.carbpol.2019.115331
• Mullin ,B. A., Abawi, G. S., Pastor-Corrales, M. A. (1991). Modification of resistance expression of Phaseolus vulgaris to Meloidogyne incognita by elevated soil temperatures. Journal of Nematology, 23(2): 182-187.
• Nico, A.I., Rafael , R.M., Jimenez-Diaz , M., Castillo P. (2004). Control of root-knot nematodes by composted agroindustrial wastes in potting mixtures. Crop Protec-tion, 23: 581-587.
• Oka, Y., Nacar, S., Putievsky, E., Ravid, U., Yaniv, Z., Spiegel, Y. (2000). Nematicidal activity of essential oils and their components against the root-knot nematode. Phytopathology, 90(7): 710-715.
• Oliveira, R. D., Dhingra, O. D., Lima, A. O., Jham, G. N., Berhow, M. A., Holloway, R. K., Vaughn, S. F. (2011). Glucosinolate content and nematicidal activity of Brazilian wild mustard tissues against Meloidogyne incognita in tomato. Plant and Soil, 341(1): 155-164.
• Ozdemir, E., Gozel, U. (2018). Nematicidal activities of essential oils against Meloidogyne incognita on tomato plant. Fresenius Environmental Bulletin, 27(6): 4511-4517.
• Park, E. J., Jang, H. J., Park, J. Y., Yang, Y. K., Kim, M. J., Park, C. S., Rho, M. C. (2023). Efficacy evaluation of Streptomyces nigrescens KA-1 against the root-knot nematode Meloidogyne incognita. Biological Control, 105150; DOI: 10.1016/j.biocontrol.2023.105150
• Randhawa, N., Sharma S.K. (2007). Control of root-knot nematode (Meloidogyne incognita) in nursery beds of tomato by soil amendment with Brassica rapa, Brassica juncea, Brassica napus and Eruca sativa plants. Pakistan Journal of Nematology, 26(1): 91-95.
• Regaieg, H., Bouajila, M., Hajji, L., Larayadh, A., Chiheni, N., Guessmi-Mzoughi, I., Horrigue-Raouani, N. (2017). Evaluation of pomegranate (Punica granatum L. var. Gabsi) peel extract for control of root-knot nematode Meloidogyne javanica on tomato. Archives of Phytopathology and Plant Protection, 50(17-18): 839-849.
• Saad, M. M. G., Abou-Taleb, H. K., Abdelgaleil, S. A. M. (2018). Insecticidal activities of monoterpenes and phenylpropenes against Sitophilus oryzae and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases. Applied Entomology and Zoology, 53(2): 173–181.
• Schlaeppi , K., Abou-Mansour, E., Buchala, A., Mauch F. (2010). Disease resistance of Arabidopsis to Phytophthora brassicae is established by the equential action of indole glucosinolates and camalexin. Plant Journal, 62: 840–851.
• Seeram, N.P., Schulman, R.N., Heber, D. (2006). Pomegranates: ancient roots to modern medicine. Boca Raton (FL): CRC Press Taylor & Francis Group, 262 pp.
• Suteu, D., Rusu, L., Zaharia, C., Badeanu, M., Daraban, G.M. (2020). Challenge of utilization vegetal extracts as natural plant protection products. Applied Sciences, 10: 1-21.
• Tariq-Khan, M., Munir, A., Mukhtar, T., Hallmann, J., Heuer, H. (2017). Distribution of root-knot nematode species and their virulence on vegetables in northern temperate agro-ecosystems of the Pakistani administered territories of Azad Jammu and Kashmir. Journal of Plant Diseases and Protection, 124: 201-212.
• Tosun, B., Göze özdemir, F.G., Şanlı, A., Karadoğan, T., Cirit, Y. (2018). Determination of nematicidal activity of some essential oils against Root knot nematode (Melo-idogyne incognita) on sugarbeet (Beta vulgaris saccharifera L.). 6th ASM International Congress of Ag-riculture and Environment, Antalya, Turkey, 127-135.
• Youssef, M. M. A., Lashein, A. M. S. (2013). Effect of cab-bage (Brassica oleracea) leaf residue as a biofumigant, on root knot nematode, Meloidogyne incognita infecting tomato. Journal of Plant Protection Research, 53(3): 271-274.
• Youssef, M.M.A., El-Nagdi, W.M.A., Eissa, M.F.M. (2014). Population density of root knot nematode, Meloidogyne incognita infecting date palm under stress of aqueous extracts of some botanicals and a commercial bacterial by product. Middle East Journal of Applied Science, 4(4): 802–805.
• Zasada, I. A., Ferris, H. (2004). Nematode suppression with brassicaceous amendments: application based upon glucosinolate profiles. Soil Biology and Biochemistry, 36(7): 1017-1024.