Farklı Bitkiler Üzerinde Yetiştirilen Tetranychus urticae (Acari: Tetranychidae)’nin Esteraz ve Glutathion S-Transferaz Enzim Düzeylerinin Belirlenmesi

Sibel Yorulmaz Salman; Fatih Bayrak; Rıfat Kılıçoğlu; İdris Sancar; Recep Ay

Determination of Esterase and Glutathione S-Transferase Enzyme Levels of Tetranychus urticae (Acari: Tetranychidae) Reared on Different Plants

Year 2013, Volume: 4 Issue: 1, 1 - 6, 01.06.2013

Sibel Yorulmaz Salman Fatih Bayrak Rıfat Kılıçoğlu İdris Sancar Recep Ay

Abstract

Bu çalışmada Tetranychus urticae’nin farklı bitkilerde yetiştirilmesi sonucu esteraz ve glutathion-S-transferaz (GST) enzim miktarlarındaki değişiklikler incelenmiştir. Konukçu bitki olarak fasulye (Phaseolius vulgaris), hıyar (Cucumis sativus) , domates (Lycopersicon esculentum), biber (Capsicum annuum), patlıcan (Solanum melongena) ve kabak (Cucurbita pepo) bitkileri kullanılmıştır. Farklı bitkiler üzerinde yetiştirilen T.urticae popülasyonlarında GST enzimi kinetik yöntemle, esteraz enzimi hem elektroforez hem de kinetik yöntemlerle belirlenmiştir. Esteraz enziminin elektroforetik olarak incelenmesi sonucunda en yoğun bant kalınlığı kabak ve patlıcanda yetiştirilen popülasyonlarda, en az bant kalınlığı ise domates ve biber üzerinde yetiştirilen popülasyonlarda belirlenmiştir. Esteraz ve GST enzim aktiviteleri sırasıyla 13.53 ve 3.68 mOD -1 min -1 mg değerleri ile kabak bitkisinde yetiştirilen popülasyonda en yüksek bulunmuştur. Biber bitkisinde yetiştirilen popülasyonda ise esteraz ve GST enzim aktiviteleri 5.83 ve 1.36 mOD -1 min -1 mg değerleri ile en düşük olarak belirlenmiştir.

Keywords

Tetranychus urticae, sebze, esteraz, glutathion-S-transferaz, detoksifikasyon enzimleri

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