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Aphanius anatoliae sureyanus (Neu, 1937) (Osteichthyes: Cyprinodontidae) Solungaç Glikokonjugatlarının Histokimyasal Özellikleri

Year 2010, Volume: 1 Issue: 1, 1 - 8, 01.06.2010

Dilek Diler Kenan Çınar

Abstract

The characteristics of glycoconjugates in the mucous cells of the Aphanius sureyanus (Neu, 1937) gill were investigated histochemica lly with the help of conventional methods. It was determined that Mucous cells ,which existed in the gill arch and primary filament epithelium contained acidic, neutral glycoconjugates, sialic acid residues and sulphated glycoconjugates and also AB pH 2.5 positive glycoconjugates were dominant in staining procedures of PAS/ AB pH 2.5 and AF/ AB pH 2.5 . It was detected that mucous cells of secondary lamellae did not contain any glycoconjugates (acidic, neutral, sialic acid residues, sulphated, very sulphated and O- sulphate esters glycoconjugates).

Keywords

Gill glycoconjugates mucous cells histochemistry Aphanius sureyanus

References

  • Arellano, J. M., Storch, V., & Sarasquete, C. (2004). Ultrastructure and histochemical study on gills and skin of the Senegal sole Solea senegalensis. Journal of Applied Ichthyology, 20, 452-460.
  • Calabró, C., Albanese, M. P., Lauriano, E. R., Licata, A., Martella, S., & Concetta, C. (2005). Morphological histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus. Folia Histochemica Et Cytobiologica, 43, 51-56.
  • Carmona, R., Garcia-Gallego, M., Sanz, A., Domezain, A., & Ostos-Garrido, M. V. (2004). Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructural modifications in seawater-acclimated specimens. Journal of Fish Biology, 64, 553-566.
  • Culling, C. F. A., Reid, P. E., & Dunn, W. L. (1976). A new histochemical. method for the identification and visualisation of both side chain acylated and non-acylated sialic acids. Journal of Histochemistry & Cytochemistry, 24, 1225-1230.
  • Çınar, K., Şenol, N., & Özen, M. R. (2008). Histochemical characterization of glycoproteins in the gills of the carp (Cyprinus carpio). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55, 61-64.
  • Díaz, A. O., Garcia, A. M., Devincenti, C. V., & Goldemberg, A. L. (2001). Mucous cells in Micropogonias furnieri gills: Histochemistry and ultrastructure. Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 30, 135-139.
  • Díaz, A. O., García, A. M., Devincenti, C. V., & Goldemberg, A. L. (2005a). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). Scientia Marina, 34, 117-122.
  • Díaz, A. O., García, A. M., Devincenti, C. V., & Goldemberg, A. L. (2005b). Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 69, 545-553.
  • Evans, D. H., Piermarini, P. M., & Potts, W. T. W. (1999). Ionic transport in the fish gill epithelium. Journal of Experimental Zoology, 283, 641-652.
  • Fletcher, T. C. (1978). Defence mechanism in fish. Journal of Histochemistry & Cytochemistry, 32, 681-689.
  • Fosket, K. J., Bern, H. A., Machen, T. E., & Conner, M. (1983). Chloride cells and the hormonal control of teleost fish osmoregulation. Journal of Experimental Biology, 106, 255-281.
  • Gomori, G. (1952). Gomori’s aldehyde fuchsin stain. In C. F. A. Culling, R. T. Allison, & W. T. Barr, (Eds.), Cellular pathology technique (p. 238). Butterworths: London.
  • Goss, G. G., Perry, S. F., Fryer, J. N., & Laurent, P. (1998). Gill morphology and acid–base regulation in freshwater fishes- effects of environmental pH change and sodium uptake blockade. Comparative Biochemistry and Physiology A, 119, 107-115.
  • Laurent, P. (1984). Gill internal morphology. In W. S. Hoar, & D. J. Randall (Eds.), Fish physiology, gills. Anatomy gas transfer and acid regulation (pp 73-183). Academic press: New York, London.
  • Laurent, P., Dunel- Erb, S., Chevalier, C., & Lignon, J. (1994). Gill epithelial cell kinetics in a freshwater teleost Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatment. Fish Physiology and Biochemistry, 3, 353- 370.
  • Lev, R., & Spicer, S. S. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry & Cytochemistry, 12, 309-309.
  • McManus, J. F. A. (1948). Histological and histochemical uses of periodic acid. Stain Technology, 23, 99-108.
  • Mowry, R. W. (1956). Alcian blue techniques for the histochemical study of acidic carbohydrates. Journal of Histochemistry & Cytochemistry, 4, 407-408.
  • Shephard, K. L. (1994). Functions for fish mucus. Reviews in Fish Biology and Fisheries, 4, 401-429.
  • Spicer, S. S., & Mayer, D. R. (1960). Aldehyde fuchsin/alcian blue. In C. F. A. Culling, R. T. Allison, & W. T. Barr (Eds.), Cellular pathology technique (p. 233). Butterworths: London.
  • Strous, G. J., & Dekker, J. (1992). Mucin-type glycoproteins. Critical Reviews in Biochemistry and Molecular Biology, 27, 57-92.
  • Vigliano, F. A., Aleman, N., Quiroga, M. I., & Nieto, J. M. (2006). Ultrastructural characterization of gills in juveniles of the Argentinian Silverside, Odontesthes bonariensis (Valenciennes, 1835) (Teleostei: Atheriniformes). Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 35, 76–83.
  • Zayed, A. E., & Mohamed, S. A. (2004). Morphological study on the gills of two species of fresh water fishes: Oreochromis niloticus and Clarias gariepinus. Annals of Anatomy, 186, 295-304.

Histochemical Characterization of Glycoconjugates in the Gills of the Aphanius sureyanus anatoliae sureyanus (Neu, 1937) (Osteichthyes: Cyprinodontidae)

Year 2010, Volume: 1 Issue: 1, 1 - 8, 01.06.2010

Dilek Diler Kenan Çınar

Abstract

The characteristics of glycoconjugates in the mucous cells of the Aphanius sureyanus (Neu, 1937) gill were investigated histochemica lly with the help of conventional methods. It was determined that Mucous cells ,which existed in the gill arch and primary filament epithelium contained acidic, neutral glycoconjugates, sialic acid residues and sulphated glycoconjugates and also AB pH 2.5 positive glycoconjugates were dominant in staining procedures of PAS/ AB pH 2.5 and AF/ AB pH 2.5 . It was detected that mucous cells of secondary lamellae did not contain any glycoconjugates (acidic, neutral, sialic acid residues, sulphated, very sulphated and O- sulphate esters glycoconjugates).

Keywords

Gill glycoconjugates mucous cells histochemistry Aphanius sureyanus

References

  • Arellano, J. M., Storch, V., & Sarasquete, C. (2004). Ultrastructure and histochemical study on gills and skin of the Senegal sole Solea senegalensis. Journal of Applied Ichthyology, 20, 452-460.
  • Calabró, C., Albanese, M. P., Lauriano, E. R., Licata, A., Martella, S., & Concetta, C. (2005). Morphological histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus. Folia Histochemica Et Cytobiologica, 43, 51-56.
  • Carmona, R., Garcia-Gallego, M., Sanz, A., Domezain, A., & Ostos-Garrido, M. V. (2004). Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructural modifications in seawater-acclimated specimens. Journal of Fish Biology, 64, 553-566.
  • Culling, C. F. A., Reid, P. E., & Dunn, W. L. (1976). A new histochemical. method for the identification and visualisation of both side chain acylated and non-acylated sialic acids. Journal of Histochemistry & Cytochemistry, 24, 1225-1230.
  • Çınar, K., Şenol, N., & Özen, M. R. (2008). Histochemical characterization of glycoproteins in the gills of the carp (Cyprinus carpio). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55, 61-64.
  • Díaz, A. O., Garcia, A. M., Devincenti, C. V., & Goldemberg, A. L. (2001). Mucous cells in Micropogonias furnieri gills: Histochemistry and ultrastructure. Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 30, 135-139.
  • Díaz, A. O., García, A. M., Devincenti, C. V., & Goldemberg, A. L. (2005a). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). Scientia Marina, 34, 117-122.
  • Díaz, A. O., García, A. M., Devincenti, C. V., & Goldemberg, A. L. (2005b). Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 69, 545-553.
  • Evans, D. H., Piermarini, P. M., & Potts, W. T. W. (1999). Ionic transport in the fish gill epithelium. Journal of Experimental Zoology, 283, 641-652.
  • Fletcher, T. C. (1978). Defence mechanism in fish. Journal of Histochemistry & Cytochemistry, 32, 681-689.
  • Fosket, K. J., Bern, H. A., Machen, T. E., & Conner, M. (1983). Chloride cells and the hormonal control of teleost fish osmoregulation. Journal of Experimental Biology, 106, 255-281.
  • Gomori, G. (1952). Gomori’s aldehyde fuchsin stain. In C. F. A. Culling, R. T. Allison, & W. T. Barr, (Eds.), Cellular pathology technique (p. 238). Butterworths: London.
  • Goss, G. G., Perry, S. F., Fryer, J. N., & Laurent, P. (1998). Gill morphology and acid–base regulation in freshwater fishes- effects of environmental pH change and sodium uptake blockade. Comparative Biochemistry and Physiology A, 119, 107-115.
  • Laurent, P. (1984). Gill internal morphology. In W. S. Hoar, & D. J. Randall (Eds.), Fish physiology, gills. Anatomy gas transfer and acid regulation (pp 73-183). Academic press: New York, London.
  • Laurent, P., Dunel- Erb, S., Chevalier, C., & Lignon, J. (1994). Gill epithelial cell kinetics in a freshwater teleost Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatment. Fish Physiology and Biochemistry, 3, 353- 370.
  • Lev, R., & Spicer, S. S. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry & Cytochemistry, 12, 309-309.
  • McManus, J. F. A. (1948). Histological and histochemical uses of periodic acid. Stain Technology, 23, 99-108.
  • Mowry, R. W. (1956). Alcian blue techniques for the histochemical study of acidic carbohydrates. Journal of Histochemistry & Cytochemistry, 4, 407-408.
  • Shephard, K. L. (1994). Functions for fish mucus. Reviews in Fish Biology and Fisheries, 4, 401-429.
  • Spicer, S. S., & Mayer, D. R. (1960). Aldehyde fuchsin/alcian blue. In C. F. A. Culling, R. T. Allison, & W. T. Barr (Eds.), Cellular pathology technique (p. 233). Butterworths: London.
  • Strous, G. J., & Dekker, J. (1992). Mucin-type glycoproteins. Critical Reviews in Biochemistry and Molecular Biology, 27, 57-92.
  • Vigliano, F. A., Aleman, N., Quiroga, M. I., & Nieto, J. M. (2006). Ultrastructural characterization of gills in juveniles of the Argentinian Silverside, Odontesthes bonariensis (Valenciennes, 1835) (Teleostei: Atheriniformes). Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 35, 76–83.
  • Zayed, A. E., & Mohamed, S. A. (2004). Morphological study on the gills of two species of fresh water fishes: Oreochromis niloticus and Clarias gariepinus. Annals of Anatomy, 186, 295-304.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Research Paper
Authors

Dilek Diler This is me

Kenan Çınar This is me

Publication Date June 1, 2010
Published in Issue Year 2010 Volume: 1 Issue: 1

Cite

APA Diler, D., & Çınar, K. (2010). Histochemical Characterization of Glycoconjugates in the Gills of the Aphanius sureyanus anatoliae sureyanus (Neu, 1937) (Osteichthyes: Cyprinodontidae). Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 1(1), 1-8.