DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvae (Nematoda) in Sander Luciopercafrom Lake Eğirdir

Year 2023, Volume: 14 Issue: 2, 346 - 354, 31.12.2023

Kevser Öztürk , Mehmet Oğuz Öztürk

https://doi.org/10.29048/makufebed.1379991

Cited By: 1

Abstract

Bu çalışmada, 3Eustrongylides excisus örneğinden 18S ve 28SrDNA gen lokuslarına ait 926-954 bp kısmi belirgin nükleotid dizileri özdeş olarak tanımlanmış ve GenBank’ta kayıt altına alınmıştır (OP480437-39). Eustrongylides excisus örneklerinin 18S ve 28SrDNA gen dizilerindeki nükleotid değerleri tanımlanmıştır: % 26,94-27,26 A; %26,78-26,84 T; % 28,30-28,62 G; % 17,39-17,92 C. Pairwise comparison metodu ile elde edilen dizi veri kümesi mesafe matrisine göre, bu çalışmada tanımlanan Eustrongylides excisus izolatları (OP480437-39) ile GenBank’ta kayıtlı Eustrongylides excisus izolatları (MK007967, MT415236, MK545494) arasında tam bir uyum görülmüştür (%0,0). Bu Eustrongylides excisus izolatları Maximum likelihood filogram analizinde aynı kümede toplanmıştır. İzolatların internal transkripsiyonlu ayırıcı (ITS) bölgesi gen sekansı sonuçları, Eustrongylides excisus'un anatomik ve morfolojik özelliklerine göre tanımlanan taksonomik konumunu doğrulamıştır. Ayrıca, Eğirdir Gölü’ndeki Eustrongylides excisus türüne ait 3 örnek üzerinden 18S ve 28SrDNA gen dizileri ilk defa bu çalışmada tanımlanmış ve bu türe ait genetik özelliklerin belirlenmesi çalışmalarına katkı yapılmıştır.

Keywords

Eustrongylides excisus, DNA sekanslama, Sander lucioperca

Supporting Institution

Afyon Kocatepe University Scientific Research Projects Unit

Project Number

21.Fen.Bil.25

Thanks

This study was supported by Afyon Kocatepe University Scientific Research Projects Unit within the scope of project number 21.Fen.Bil.25.

DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Abstract

In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank (OP480437-39). Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94% 27.26 A; 26.78%-26.84% T; 28.30-28.62% G; 17.39-17.92% C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement (0%) between the Eustrongylides excisus isolates identified in this study (OP480437-39) and the Eustrongylides excisus isolate registered in the GenBank (MK007967, MT415236, MK545494).The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer (ITS) region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.

Keywords

Eustrongylides excisus, DNA sequencing, Sander lucioperca

Project Number

21.Fen.Bil.25

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