Effect of Combinations of Salt and Temperature on Morphological Characteristics of Microfungi

Year 2018, Volume: 9 Issue: 2, 158 - 164, 25.10.2018

Rasime Demirel , Orkun Kayış Semra İlhan

Abstract

Aim of this study is investigation of effects of different salt and
temperature combinations on morphological characteristics of microfungi that
were isolated and identified with traditional and molecular methods from
Çamaltı Saltern/İzmir.  A total of 37
species as 3 species belong to Alternaria,
13 Aspergillus, 2 Chaetomium, 5 Cladosporium, 9 Penicillium,
and 1 species belong to each species as Acremonium,
Arthrinium, Biscogniaxua, Diaporthe, Fusarium were used in this study.
Using aseptic techniques, each of the microfungi were inoculated on Mat Extract
Agar medium that was added 8%, 16%, 24% NaCI. Each concentration media was
incubated at 17, 27, 37 ℃ at 7 days. After incubation of each cultures were investigated spore
formation, mycelium diameter, colony front and back colour, colony texture,
colony diameters, sclerotium and cleistothecium. All results were collected in a
table and photographed. As a result of the study, we showed that
combination of different NaCl concentrations and temperatures have affected on
colony properties. Furthermore, these effects revealed morphological
differences between closely related fungi such as members of section Versicolor, Aspergillus, Clavati, Circumdati, Flavi, Penicillium
subgenus Penicillium.

Keywords

Salt, Temperature, Microfungi, Combine effect

Mikrofungusların Morfolojik Karakterleri Üzerine Tuz Ve Sıcaklık Kombinasyonlarının Etkisi

Abstract

Bu çalışmanın amacı, farklı tuz ve sıcaklık kombinasyonlarının Çamaltı Tuzlası/
İzmir'den izole edilen, geleneksel ve moleküler yöntemlerle tanımlanmış
mikrofungusların morfolojik karakterleri üzerindeki etkilerinin
araştırılmasıdır. Bu çalışmada 3 Alternaria,
13 Aspergillus, 2 Chaetomium, 5 Cladosporium, 9 Penicillium
ve Acremonium, Arthrinium, Biscogniaxua,
Diaporthe, Fusarium cinlerinin her birinden 1’er tane olmak üzere toplam 37
tür kullanılmıştır. Aseptik teknikler kullanılarak, mikrofungusların her biri,
%8, %16, %24 NaCI eklenmiş Mat Ekstrakt Agar plaklarına aşılanmıştır. Her
konsantrasyon ortamı 17, 27, 37℃'de 7 gün boyunca inkübe edilmiştir. Her
kültürün inkübasyonundan sonra spor oluşumu, miselyum çapı, koloni ön ve arka
rengi, koloni dokusu, koloni çapı, sklerotium ve kleistothecium varlığı
incelenmiştir. Bütün sonuçlar bir tabloda toplanmış ve fotoğraflanmıştır.
Çalışmanın sonucunda, farklı NaCl konsantrasyonu ve sıcaklık kombinasyonlarının
koloni özelliklerine etki ettiği gösterilmiştir. Ayrıca, bu etkiler, Versicolor, Aspergillus, Clavati, Circumdati, Flavi, Penicillium
subgenus Penicillium üyeleri gibi
yakın ilişkili mikrofunguslar arasında morfolojik farklılıklar ortaya
çıkarmıştır.

Keywords

Tuz, Sıcaklık, Mikrofungi, Kombine etki

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