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Tricholoma anatolicum ve Tricholoma caligatum’un Morfolojik ve Moleküler Yönden Karşılaştırılması

Year 2019, Volume: 10 Issue: 3, 34 - 40, 26.12.2019

Abstract

Tricholoma cinsi morfolojik karakterler bakımından diğer cinslerden kolaylıkla
ayırt edilebilmesine karşın, yakın morfolojik karakterler bakımından tür
düzeyinde tayini oldukça zor bir cinstir. Bu çalışma ile
ekonomik değere sahip
ve birbirleriyle oldukça benzer T. caligatum ve T. anatolicum türleri arasındaki akrabalık ilişkileri morfolojik ve
moleküler açıdan ele alınmıştır. Genetik ilişkiler ISSR markırları yardımıyla
değerlendirilmiştir. Elde edilen dendograma göre T. caligatum ve T. anatolicum
türlerinin birbirlerine % 51 oranında benzerlik gösterdiği tespit edilmiştir.
Sonuç olarak, bu iki türü birbirinden kolayca ayırt etmek için burada
morfolojik özellikler ve moleküler belirteçler önerilmiştir.

Supporting Institution

Selçuk Üniversitesi BAP Koordinatörlüğü

Project Number

10201093

Thanks

Selçuk Üniversitesi BAP koordinatörlüğüne (Proje Numarası: 10201093) finansal desteği için teşekkür ederiz.

References

  • Allı, H. and Şen İ. (2016). Tricholoma Türlerinin Yenilebilirliği Üzerine Notlar. Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 4(3): 178-181.Anderson, I. C., Chambers, S. M. and Cairney, J.W. G. (2001). Distribution and persistence of Australian Pisolithus species genets at native sclerophyll forest field sites. Mycol Res, 105: 971–976.Amend, A., Garbelotto, M., Fang, Z. and Keeley, S. (2010). Isolation by landscape in populations of a prized edible mushroom Tricholoma matsutake. Conserv Genet, 11: 795–802.Amicucci, A., Zambonelli, A., Guidi, C. and Stocchi, V. (2001). Morphological and molecular characterisation of Pulvinula constellatio ectomycorrhizae. FEMS Microbiology Letters 194: 121-125.Bao, D., Koike, A., Yao, F., Yamanaka, K., Aimi, T. and Kitamoto, Y. (2007). Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia. J Wood Sci 53: 344–350.Bergius, N., Danell, E. (2000). The Swedish matsutake (Tricholoma nauseosum syn. T. matsutake): distribution, abundance and ecology. Scand J For Res, 15: 318–325.Bessette, E. A., Bessette, A. R., Roody, W.C., Trudell, S. A. (2013). Tricholomas of North America, A Mushroom Field Guide. Austin. University of Texas Press.Boa, E. (2004). Wild edible fungi, a global overview of their use and importance to people. Rome, Non Wood Forest Products 17. FAO.Chapela, I. H. and Garbelotto, M. (2004). Phylogeography and evolution in matsutake and close allies inferred by analyses of ITS sequences and AFLPs. Mycologia 96: 730–741.Christensen, M. and Heilmann-Clausen, J. (2013). The genus Tricholoma, Fungi of Northern Europe, Vol 4. Denmark. Narayana Press.Cullings, K.W. (1992). Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology, 1: 233–240.Doğan H. H. and Akata I. (2011). Ecological features of Tricholoma anatolicum in Turkey. African Journal of Biotechnology, 10 (59): 12626-12668.Doyle, J. J. and Doyle J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, Botanical Society of America, 19: 11–15.Galli R. (1999). I Tricholomi. Milano. Dalla Natura.Gherbi, H., Delaruelle, C., Selosse, M.A. and Martin, F. (1999). High genetic diversity in a population of the ectomycorrhizal basidiomycete Laccaria amethystina in a 150-year-old beech forest. Mol Ecol, 8: 2003–2013.Horton, T.R. and Bruns, T.D. (2001). The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Molecular Ecology 10: 1855-1871.Intini, M., Dogan, H. H. and Riva, A. (2003). Tricholoma anatolicum spec. nov.: un nuovo membro del gruppo matsutake. Micol Veg Mediterr, 18:135–142.Kalmış, E., Eltem R., Işıloğlu, M., Solak, M. H., Kalyoncu, F. ve Gezgin, Y., 2009, Muğla ilindeki Tricholoma caligatum populasyonlarının belirlenmesi ile İnvitro da kültürel özelliklerinin açığa çıkarılması. TÜBİTAK Projesi Ege Üniversitesi Fen Bilimleri Enstitüsü (Tübitak-TBAG, 105T128), İzmir.Kaya, A., Uzun, Y. ve Karacan, İ. H. (2009). Göksun (Kahramanmaraş) Yöresi Makrofungusları. Türk J. Bot, 33: 131-139.Lim, S. R., Fischer, A., Berbee, M. and Berch, S. M. (2003). Is the booted Tricholoma in British Columbia really Japanese matsutake? BC J Ecosyst Manage, 3:1–7.Matsushita, N., Kikuchi, K., Sasaki, Y., Guerin-Laguette, A., Lapeyrie, F., Vaario, L-M., Intini, M. and Suzuki, K. (2005). Genetic relationship of Tricholoma matsutake and T.nauseosum from the northern hemisphere based on analyses of ribosomal DNA spacer regions. Mycoscience 46: 90–96.Ota, Y., Yamanaka, T., Murata, H., Neda, H., Ohta, A., Kawai, M., Yamada, A., Konno, M. and Tanaka, C. (2012). Phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses. Mycologia, 104 (6): 1369–1380. Riva, A. (2003). Tricholoma (Fr.) Staude Supplemento, Fungi Europaei. Italia. Candusso.Rohlf, F. J. (1998). NTSYSpc numerical taxonomy and multivariate analysis system user guide, Exeter Software, New York, 0-925031-28-3.Sawyer, N.A. and Chambers, S. M. and Cairney, J. W. G. (2001). Distribution and persistence of Amanita muscaria genotypes in Australian Pinus radiata plantations. Mycol Res, 105: 966–970.Soltis, D.E., Collõer, T.G., Edgerton, M.L. 1991. The Heuchera group (Saxifragaceae): Evidence for chloroplast transfer and paraphyly. Amer J Bot, 78: 1091–1112.Taşkın H., Eker T., Bozok F., Doğan H.H., Büyükalaca S. (2018). Determination of Multiple Antioxidant Activities of Endemic Tricholoma anatolicum H.H Doğan & Intini Collected from Turkey. Turkish Journal of Agriculture - Food Science and Technology, 6(11): 1582–1585. Xu, J., Sha, T., Li, Y. C., Zhao, Z. W. and Yang, Z. L. (2008). Recombination and genetic differentiation among natural populations of the ectomycorrhizal mushroom Tricholoma matsutake from southwestern China. Mol Ecol, 17: 1238–1247.Wan, J., Koike, A., Yamanaka, K., Sotome, K., Morinaga, T., Tanaka, C., Terashima, Y. and Aimi T. (2012). Genetic diversity of Tricholoma matsutake and close allies associated with broad- leaved trees in Asia. Mushroom Sci Biotechol 19: 167–174.Zhou, Z., Miwa, M., Hogetsu, T. (1999). Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytol, 144: 55–63.Zhou, Z., Miwa M., Hogetsu, T. (2000). Genet distribution of ectomycorrhizal fungus Suillus grevillei populations in two Larix kaempferi stands over two years. J Plant Res, 113: 365–374.Zietkiewicz, E., Rafalski, A., Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183.

The Comparison of Tricholoma anatolicum and Tricholoma caligatum Species by Morphological and Molecular Methods

Year 2019, Volume: 10 Issue: 3, 34 - 40, 26.12.2019

Abstract

Although the genus Tricholoma can be easily distinguished
from other genera with morphological characters, it is complicated to determine
at the species level in terms of close morphological characters. Within this
study, the relationships between T.
caligatum
and T. anatolicum
species which have economical value and are very similar to each other are
discussed in terms of morphological and molecular point of view. Genetic
relationships were evaluated with ISSR markers. According to the obtained
dendograms, it was found that T. caligatum
and T. anatolicum species were
similar to each other at a rate of 51%. In conclusion, morphological traits and
molecular markers are proposed here to easily distinguish these two species
from each other.

Project Number

10201093

References

  • Allı, H. and Şen İ. (2016). Tricholoma Türlerinin Yenilebilirliği Üzerine Notlar. Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 4(3): 178-181.Anderson, I. C., Chambers, S. M. and Cairney, J.W. G. (2001). Distribution and persistence of Australian Pisolithus species genets at native sclerophyll forest field sites. Mycol Res, 105: 971–976.Amend, A., Garbelotto, M., Fang, Z. and Keeley, S. (2010). Isolation by landscape in populations of a prized edible mushroom Tricholoma matsutake. Conserv Genet, 11: 795–802.Amicucci, A., Zambonelli, A., Guidi, C. and Stocchi, V. (2001). Morphological and molecular characterisation of Pulvinula constellatio ectomycorrhizae. FEMS Microbiology Letters 194: 121-125.Bao, D., Koike, A., Yao, F., Yamanaka, K., Aimi, T. and Kitamoto, Y. (2007). Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia. J Wood Sci 53: 344–350.Bergius, N., Danell, E. (2000). The Swedish matsutake (Tricholoma nauseosum syn. T. matsutake): distribution, abundance and ecology. Scand J For Res, 15: 318–325.Bessette, E. A., Bessette, A. R., Roody, W.C., Trudell, S. A. (2013). Tricholomas of North America, A Mushroom Field Guide. Austin. University of Texas Press.Boa, E. (2004). Wild edible fungi, a global overview of their use and importance to people. Rome, Non Wood Forest Products 17. FAO.Chapela, I. H. and Garbelotto, M. (2004). Phylogeography and evolution in matsutake and close allies inferred by analyses of ITS sequences and AFLPs. Mycologia 96: 730–741.Christensen, M. and Heilmann-Clausen, J. (2013). The genus Tricholoma, Fungi of Northern Europe, Vol 4. Denmark. Narayana Press.Cullings, K.W. (1992). Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology, 1: 233–240.Doğan H. H. and Akata I. (2011). Ecological features of Tricholoma anatolicum in Turkey. African Journal of Biotechnology, 10 (59): 12626-12668.Doyle, J. J. and Doyle J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, Botanical Society of America, 19: 11–15.Galli R. (1999). I Tricholomi. Milano. Dalla Natura.Gherbi, H., Delaruelle, C., Selosse, M.A. and Martin, F. (1999). High genetic diversity in a population of the ectomycorrhizal basidiomycete Laccaria amethystina in a 150-year-old beech forest. Mol Ecol, 8: 2003–2013.Horton, T.R. and Bruns, T.D. (2001). The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Molecular Ecology 10: 1855-1871.Intini, M., Dogan, H. H. and Riva, A. (2003). Tricholoma anatolicum spec. nov.: un nuovo membro del gruppo matsutake. Micol Veg Mediterr, 18:135–142.Kalmış, E., Eltem R., Işıloğlu, M., Solak, M. H., Kalyoncu, F. ve Gezgin, Y., 2009, Muğla ilindeki Tricholoma caligatum populasyonlarının belirlenmesi ile İnvitro da kültürel özelliklerinin açığa çıkarılması. TÜBİTAK Projesi Ege Üniversitesi Fen Bilimleri Enstitüsü (Tübitak-TBAG, 105T128), İzmir.Kaya, A., Uzun, Y. ve Karacan, İ. H. (2009). Göksun (Kahramanmaraş) Yöresi Makrofungusları. Türk J. Bot, 33: 131-139.Lim, S. R., Fischer, A., Berbee, M. and Berch, S. M. (2003). Is the booted Tricholoma in British Columbia really Japanese matsutake? BC J Ecosyst Manage, 3:1–7.Matsushita, N., Kikuchi, K., Sasaki, Y., Guerin-Laguette, A., Lapeyrie, F., Vaario, L-M., Intini, M. and Suzuki, K. (2005). Genetic relationship of Tricholoma matsutake and T.nauseosum from the northern hemisphere based on analyses of ribosomal DNA spacer regions. Mycoscience 46: 90–96.Ota, Y., Yamanaka, T., Murata, H., Neda, H., Ohta, A., Kawai, M., Yamada, A., Konno, M. and Tanaka, C. (2012). Phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses. Mycologia, 104 (6): 1369–1380. Riva, A. (2003). Tricholoma (Fr.) Staude Supplemento, Fungi Europaei. Italia. Candusso.Rohlf, F. J. (1998). NTSYSpc numerical taxonomy and multivariate analysis system user guide, Exeter Software, New York, 0-925031-28-3.Sawyer, N.A. and Chambers, S. M. and Cairney, J. W. G. (2001). Distribution and persistence of Amanita muscaria genotypes in Australian Pinus radiata plantations. Mycol Res, 105: 966–970.Soltis, D.E., Collõer, T.G., Edgerton, M.L. 1991. The Heuchera group (Saxifragaceae): Evidence for chloroplast transfer and paraphyly. Amer J Bot, 78: 1091–1112.Taşkın H., Eker T., Bozok F., Doğan H.H., Büyükalaca S. (2018). Determination of Multiple Antioxidant Activities of Endemic Tricholoma anatolicum H.H Doğan & Intini Collected from Turkey. Turkish Journal of Agriculture - Food Science and Technology, 6(11): 1582–1585. Xu, J., Sha, T., Li, Y. C., Zhao, Z. W. and Yang, Z. L. (2008). Recombination and genetic differentiation among natural populations of the ectomycorrhizal mushroom Tricholoma matsutake from southwestern China. Mol Ecol, 17: 1238–1247.Wan, J., Koike, A., Yamanaka, K., Sotome, K., Morinaga, T., Tanaka, C., Terashima, Y. and Aimi T. (2012). Genetic diversity of Tricholoma matsutake and close allies associated with broad- leaved trees in Asia. Mushroom Sci Biotechol 19: 167–174.Zhou, Z., Miwa, M., Hogetsu, T. (1999). Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytol, 144: 55–63.Zhou, Z., Miwa M., Hogetsu, T. (2000). Genet distribution of ectomycorrhizal fungus Suillus grevillei populations in two Larix kaempferi stands over two years. J Plant Res, 113: 365–374.Zietkiewicz, E., Rafalski, A., Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183.
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Details

Primary Language Turkish
Journal Section 2nd International Eurasian Mycology Congress (EMC’ 19)
Authors

Meryem Bozkurt 0000-0003-0338-0849

Şenay İlban This is me

Sinan Aktaş 0000-0003-1657-5901

Tuna Uysal 0000-0001-9968-5633

Project Number 10201093
Publication Date December 26, 2019
Published in Issue Year 2019 Volume: 10 Issue: 3

Cite

APA Bozkurt, M., İlban, Ş., Aktaş, S., Uysal, T. (2019). Tricholoma anatolicum ve Tricholoma caligatum’un Morfolojik ve Moleküler Yönden Karşılaştırılması. Mantar Dergisi, 10(3), 34-40. https://doi.org/10.30708/mantar.624086

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