Effect of Pine Oil on Antihyperglycemic and Some Biochemical Parameters in serum of Streptozotocin Induced Tip-2 Diabetic rats

Abstract

The present study was designed to evaluate the effect of pine oil on hyperglycemia and some biochemical parameters in streptozotocin-induced type- 2 diabetic rats. Rats were divided into three groups: control (C) diabetes (STZ-DM), diabetes+pine oil (STZ-DM+PO) grups. Diabetes induced in rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). 1 ml/kg the dose pine oil was intraperitoneally injected twice in a week to the diabetes+pine oil (STZ-DM+PO), plus 1 g/L dose of pure pine oil was added to drinking water of these group. According to our analysis results; fasting blood glucose levels were significantly decreased (p<0.001) in STZ-DM+PO group when compared to STZ-DM group. In addition, body weight was found to increase significantly (p<0.001) in STZ-DM+PO compared to STZ-DM group. MDA-TBA levels in serum decreased significantly (p<0.001) in STZ-DM+PO group in comparison to STZ-DM group. While compared to control group; vitamin K₂, vitamin D₃, α-tocopherol, retinol, cholesterol and sterols level were significantly (p<0.001) increased in STZ-DM group. Also when compared to STZ-DM group; vitamin K₂, α-tocopherol and sterols levels decreased significantly (p<0.001), vitamin D₃, retinol, vitamin Kand cholesterol levels increased significantly (p<0.001) in the sera of STZ-DM+PO group. According to our results; stearic acid, oleic acid, linoleic acid levels were significantly (p<0.001) increased in control groups and also stearic acid, oleic acid, linoleic acid levels decreased significantly (p<0.001), palmitoleic acid level increased significantly (p<0.001) in the serum of STZ-DM+PO group when both of the groups compared to STZ-DM group.

This study demonstrates that pine oil possesses hypoglycemic potentials in STZ induced diabetic rats and concluded that pine oil has positive effect on some biochemical parameters in the serum.

Keywords

• Hyperglycemia;pine oil;lipid peroxidation;antioxidant activity;fatty acid

Streptozotosin ile Tip-2 Diyabet Oluşturulan Sıçanlarda Çam Yağının Antihiperglisemik ve Bazı Biyokimyasal Parametrelere Etkisi

Abstract

Bu çalışma, streptozotosin ile Tip-2 diyabet oluşturulan Wistar sıçanlarda çam yağının hiperglisemi ile bazı biyokimyasal parametrelere etkisinin araştırılması için tasarlandı. Sıçanlar, kontrol (K), diyabet (STZ-DM) ve diyabet+çam yağı (STZ-DM+ÇY) olmak üzere üç gruba ayrıldı. Diyabet gruplarına intraperitoneal enjeksiyonla streptozotosin (45 mg/kg) verilerek diyabet oluşturuldu. Çam yağı grubundaki sıçanlara (STZ-DM+ÇY) haftada iki gün 1 ml/kg (v/v) dozunda intraperitoneal enjeksiyonla çam yağı ve 1 g/L düzeyinde saf çam yağı içme suyuna ilave edilerek verildi. Analiz sonuçlarına göre, STZ-DM gurubu ile karşılaştırıldığında STZ-DM+ÇY gurubunda açlık kan glikoz düzeyinin anlamlı bir şekilde azaldığı (p<0.001), vücut ağırlığının anlamlı (p<0.001) bir şekilde arttığı saptandı. STZ-DM grubuna göre STZ-DM+ÇY grubunun serumunda MDA-TBA düzeyinin anlamlı bir şekilde azaldığı (p<0.001) saptandı. Elde ettiğimiz sonuçlara göre K grubu ile karşılaştırıldığında STZ-DM grubunda vitamin K₂, vitamin D₃, α-tokoferol, retinol, kolesterol ve sterol miktarı önemli düzeyde (p<0.001) artmıştır. Ayrıca serumda STZ-DM grubuna göre STZ-DM+ÇY grubunda vitamin K₂, α-tokoferol ve sterol düzeyinin anlamlı bir şekilde (p<0.001) azaldığı, vitamin D₃, retinol, vitamin K₁ ve kolesterol düzeyinin anlamlı bir şekilde (p<0.001) arttığı saptandı. Elde ettiğimiz verilere kontrol göre STZ-DM grubunda stearik, oleik ve linoleik asit düzeyinin anlamlı (p<0.001) düzeyde arttığı tespit edildi. Ayrıca STZ-DM grubuna göre STZ-DM+ÇY grubunda stearik, oleik ve linoleik asit düzeyinin anlamlı düzeyde (p<0.001) azaldığı, palmitoleik asit düzeyinin anlamlı düzeyde (p<0.001) arttığı belirlendi.

Bu çalışma, çam yağının hipoglisemik potansiyele sahip olduğunu ve ayrıca serumda bazı biyokimyasal parametreler üzerinde olumlu etki gösterdiği tespit edildi.

Keywords

• Hiperglisemi;çam yağı;lipit peroksidasyon;antioksidan aktivite;yağ asidi

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