Effect of Pine Oil on Antihyperglycemic and Some Biochemical Parameters in serum of Streptozotocin Induced Tip-2 Diabetic rats

Öz

The present study was designed to evaluate the effect of pine oil on hyperglycemia and some biochemical parameters in streptozotocin-induced type- 2 diabetic rats. Rats were divided into three groups: control (C) diabetes (STZ-DM), diabetes+pine oil (STZ-DM+PO) grups. Diabetes induced in rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). 1 ml/kg the dose pine oil was intraperitoneally injected twice in a week to the diabetes+pine oil (STZ-DM+PO), plus 1 g/L dose of pure pine oil was added to drinking water of these group. According to our analysis results; fasting blood glucose levels were significantly decreased (p<0.001) in STZ-DM+PO group when compared to STZ-DM group. In addition, body weight was found to increase significantly (p<0.001) in STZ-DM+PO compared to STZ-DM group. MDA-TBA levels in serum decreased significantly (p<0.001) in STZ-DM+PO group in comparison to STZ-DM group. While compared to control group; vitamin K₂, vitamin D₃, α-tocopherol, retinol, cholesterol and sterols level were significantly (p<0.001) increased in STZ-DM group. Also when compared to STZ-DM group; vitamin K₂, α-tocopherol and sterols levels decreased significantly (p<0.001), vitamin D₃, retinol, vitamin Kand cholesterol levels increased significantly (p<0.001) in the sera of STZ-DM+PO group. According to our results; stearic acid, oleic acid, linoleic acid levels were significantly (p<0.001) increased in control groups and also stearic acid, oleic acid, linoleic acid levels decreased significantly (p<0.001), palmitoleic acid level increased significantly (p<0.001) in the serum of STZ-DM+PO group when both of the groups compared to STZ-DM group.

This study demonstrates that pine oil possesses hypoglycemic potentials in STZ induced diabetic rats and concluded that pine oil has positive effect on some biochemical parameters in the serum.

Anahtar Kelimeler

• Hyperglycemia;pine oil;lipid peroxidation;antioxidant activity;fatty acid

Streptozotosin ile Tip-2 Diyabet Oluşturulan Sıçanlarda Çam Yağının Antihiperglisemik ve Bazı Biyokimyasal Parametrelere Etkisi

Öz

Bu çalışma, streptozotosin ile Tip-2 diyabet oluşturulan Wistar sıçanlarda çam yağının hiperglisemi ile bazı biyokimyasal parametrelere etkisinin araştırılması için tasarlandı. Sıçanlar, kontrol (K), diyabet (STZ-DM) ve diyabet+çam yağı (STZ-DM+ÇY) olmak üzere üç gruba ayrıldı. Diyabet gruplarına intraperitoneal enjeksiyonla streptozotosin (45 mg/kg) verilerek diyabet oluşturuldu. Çam yağı grubundaki sıçanlara (STZ-DM+ÇY) haftada iki gün 1 ml/kg (v/v) dozunda intraperitoneal enjeksiyonla çam yağı ve 1 g/L düzeyinde saf çam yağı içme suyuna ilave edilerek verildi. Analiz sonuçlarına göre, STZ-DM gurubu ile karşılaştırıldığında STZ-DM+ÇY gurubunda açlık kan glikoz düzeyinin anlamlı bir şekilde azaldığı (p<0.001), vücut ağırlığının anlamlı (p<0.001) bir şekilde arttığı saptandı. STZ-DM grubuna göre STZ-DM+ÇY grubunun serumunda MDA-TBA düzeyinin anlamlı bir şekilde azaldığı (p<0.001) saptandı. Elde ettiğimiz sonuçlara göre K grubu ile karşılaştırıldığında STZ-DM grubunda vitamin K₂, vitamin D₃, α-tokoferol, retinol, kolesterol ve sterol miktarı önemli düzeyde (p<0.001) artmıştır. Ayrıca serumda STZ-DM grubuna göre STZ-DM+ÇY grubunda vitamin K₂, α-tokoferol ve sterol düzeyinin anlamlı bir şekilde (p<0.001) azaldığı, vitamin D₃, retinol, vitamin K₁ ve kolesterol düzeyinin anlamlı bir şekilde (p<0.001) arttığı saptandı. Elde ettiğimiz verilere kontrol göre STZ-DM grubunda stearik, oleik ve linoleik asit düzeyinin anlamlı (p<0.001) düzeyde arttığı tespit edildi. Ayrıca STZ-DM grubuna göre STZ-DM+ÇY grubunda stearik, oleik ve linoleik asit düzeyinin anlamlı düzeyde (p<0.001) azaldığı, palmitoleik asit düzeyinin anlamlı düzeyde (p<0.001) arttığı belirlendi.

Bu çalışma, çam yağının hipoglisemik potansiyele sahip olduğunu ve ayrıca serumda bazı biyokimyasal parametreler üzerinde olumlu etki gösterdiği tespit edildi.

Anahtar Kelimeler

• Hiperglisemi;çam yağı;lipit peroksidasyon;antioksidan aktivite;yağ asidi

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