Kriyopreservasyon oligoastenoteratozoospermik erkeklerde DNA fragmantasyonunu ve ultrastrüktürel hasarı tetikler

Year 2017, , 63 - 72, 21.05.2017

Pınar Turan Gözde Erkanlı Şentürk Feriha Ercan

https://doi.org/10.5472/marumj.344797

Cited By: 1

Abstract

Amaç: Oligoastenoteratozoospermi (OAT) erkek infertilitisi

sebeplerinden biridir. Kriyopreservasyon infertilite yönetimi

için değerlidir. Bu çalışmanın amacı, OAT’lı hastalarda

sperm motilitesi, morfolojisi, ultrastrüktürel detaylar ve DNA

fragmentasyonu üzerine kriyopreservasyonun etkilerini ortaya

çıkarmaktır.

Gereç ve Yöntem: Bu gözlemsel çalışmada, gönüllü 20

normospermik ve 20 OAT’lı hastadan ejakulatlar toplanmıştır.

Ejakulatlar, -196oC sıvı nitrojende saklanmış ve çözdürmeden üç

ve altı ay sonra analiz edilmiştir. Ejakulatlarda, motilite, morfoloji

ve DNA fragmantasyonu dondurma öncesi ve sonrasında

değerlendirilmiştir.

Bulgular: Her iki grupta da sperm motilitesi ve morfolojisi

kriyopresvasyondan etkilenmiştir. Her iki grupta da çözdürmeden

sonra morfolojik değişiklikler anlamlı artmıştır. Ultrastrüktürel

incelemeler membran bütünlüğünün değiştiğini ve akrozomal

hasarın arttığını göstermiştir. Her iki grupta da, dondurma öncesi

ile karşılaştırıldığında, çözdürme sonrasında DNA kırıkları

olan hücrelerin arttığı gözlenmiştir. Her iki dondurma-çözme

periyodunda, kontrol grubu ile kıyaslandığında OAT grubunda

DNA kırıkları olan spermatozoa sayısının anlamlı olarak daha

yüksek olduğu gözlenmiştir.

Sonuç: Kriyopreservasyon, hem normospermik hem de OAT

gruplarında ultrastrüktürel hasar yapmaktadır ve DNA hasarını

indüklemektedir. Bu hasar OAT hastalarında daha ciddidir.

Keywords

Kriyopreservasyon, Oligoastenoteratozoospermi, DNA fragmantasyonu, ultrastrüktür

Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men

Abstract

Objective: Oligoasthenoteratozoospermia (OAT) is one of the

causes of male infertility. Cryopreservation is valuable for the

management of infertility. This study aimed to reveal the effects

of cryopreservation on sperm motility, morphology, ultrastructural

details and DNA fragmentation in patients with OAT.

Materials and Methods: In this observational study,

ejaculates were collected from 40 male volunteers of whom 20

were OAT and 20 were normospermic. The ejaculates were stored

in liquid nitrogen at -196oC and analysed following thawing after

1 or 3 months. Ejaculates were evaluated in terms of motility,

morphology and DNA fragmentation before and after thawing.

Results: Sperm motility and morphology were both affected

by cryopreservation in samples from both groups. After thawing,

spermatozoa with morphological abnormalities were increased

significantly in both groups. Ultrastructural investigations showed

alteration in integrity of the membranes and increased acrosomal

defects. Post-thaw investigations revealed prominent increases

in the number of DNA fragmented cells in both groups when

compared to the results before freezing. OAT groups revealed a

significantly higher number of DNA fragmented spermatozoa

compared to the normospermic group for both time periods.

Conclusion: Cryopreservation produces ultrastructural

damage and induces DNA fragmentation in both normospermic

and OAT groups. The damage is more severe in the OAT group.

Keywords

Cryopreservation, Oligoasthenoteratozoospermia, DNA fragmentation, Ultrastructure

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