Uzun süreli inkübasyonun ve sıcaklığın oosit aktivatörü fosfolipaz C-zeta aktivitesine etkisi

Year 2017, Volume: 30 Issue: 3, 155 - 161, 29.10.2017

Elif Kervancıoglu Demırcı Mehmet Ertan Kervancıoglu Gulnaz Nural Bekıroglu Sule Cetinel

https://doi.org/10.5472/marumj.370643

Abstract

Amaç: Kapasite olmuş spermin fertilizasyon kapasitesinin

zamanla üstsel olarak düştüğü bilinmesine rağmen bunun nedeni

hala araştırılmaktadır. Çalışmada amaç; uzamış inkübasyonun

ve sıcaklığın sperm fertilizasyon kapasitesi üzerinde etkisini

hareketlilik ve oosit aktivasyon faktörü olarak kabul edilen

fosfolipaz C-zeta (PLCZ) ile değerlendirmektir.

Gereç ve Yöntem: On altı infertil erkek hastadan, ciddi

oligoasthenospermisi olmayan 11’inin semenlerine dansite

gradyan yıkaması uygulandı. Ardından örnekler bölünerek 3 gün

oda sıcaklığında veya 37°C’de kültüre edildi. Örnekler hareketlilik,

PLCZ boyanma yüzdesi ve yoğunluğu açısından günlük olarak

değerlendirildi.

Bulgular: Hem oda sıcaklığı, hem de vücut sıcaklığında her

üç parametrede de zamanla azalma gözlendi. Özellikle günler arası

hareketlilikteki değişim ile PLCZ boyanan sperm yüzdesindeki

değişim arasında güçlü bir korelasyon bulundu, ancak bu

korelasyon zamanla azalmakta idi.

Sonuç: İnkübasyon süresinin uzamasıyla, PLCZ boyanma

parametreleri ve hareketlilik arasında korelasyon görülmektedir.

PLCZ boyanmasının semen analizi ile rutinde kullanımı, özellikle

açıklanamayan infertilite ve fertilizasyon başarısızlığı vakalarının

spermlerinin fertilizasyon kapasitesinin belirlenmesinde yarar

sağlayabilecek ve yardımcı üreme teknileri (YÜT) sikluslarındaki

başarıyı artırabilecektir.

Keywords

Sıcaklık, Fosfolipaz C-zeta, Fertilizasyon kapasitesi, Sperm, Oosit aktivasyonu, Uzun süreli inkübasyon

The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm

Abstract

Objective: Fertilization capacity of capacitated sperm decreases

exponentially over time, but the reason is still under investigation.

The aim of the study was to analyze the effect of prolonged

incubation and temperature on sperm fertilization capacity with

motility and staining parameters of phospholipase C-zeta (PLCZ),

which is considered to be the oocyte activation factor.

Materials and Methods: Density gradient washing

was applied to semen of 11 infertile patients without severe

oligoasthenospermia out of 16 patients. The samples were divided

and cultured either at room temperature or at 37°C for 3 days. The

spermatozoons were evaluated for motility, PLCZ staining and

intensity daily.

Results: All parameters decreased both at room and body

temperature with increased incubation time. There was a strong

correlation between the change in motility and in the percentage

of PLCZ stained sperms, but this correlation decreased with

incubation time.

Conclusion: Prolonged incubation results show the correlation

between PLCZ staining parameters and motility. Routine use of

PLCZ staining together with semen analysis, will be useful to

predict fertilization capacity of the sperm especially for unexplained

infertility and fertilization failure cases, and also can increase the

success of assisted reproductive technologies (ART) cycles.

Keywords

Temperature, Phospholipase C zeta, Fertilization capacity, Sperm, Oocyte activaton, Prolonged incubation

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