Tadalafil attenuates spinal cord injury induced oxidative organ damage in rats

Abstract

Spinal cord injury (SCI) has been shown to cause systemic
inflammatory response syndrome (SIRS) which damages
multiple organs due to an influx of inflammatory cells from the
circulation. In this study, we evaluated the effect of tadalafil, a
phoshodiesterase inhibitor, against spinal cord, kidney and
bladder damage in experimental animal model of spinal cord
injury. Male Wistar albino rats were divided into sham-operated
control, and either vehicle or tadalafil-treated SCI groups. In
order to induce SCI, a standard weight-drop method that
induced a moderately severe injury (100 g/cm force) at T10,
was used. Injured animals were given either 10 mg/kg tadalafil
or vehicle 15 minutes post injury and repeated daily for seven
days. After decapitation spinal cord, kidney and bladder tissue
samples were obtained to examine oxidative tissue injury;
malondialdehyde (MDA) and glutathione (GSH) levels, and
superoxide dismutase (SOD), myeloperoxidase (MPO) and
caspase-3 activities. Tissues were also examined histologically.
In the injured animals, MDA levels MPO and caspase-3
activities in tissues were found significantly increased while
tadalafil treatment reversed these increases. On the other hand
SCI-induced decreases in GSH levels and SOD activities were
also reversed with tadalafil treatment. According to the results,
tadalafil exerts beneficial effects against SCI-induced
oxidative damage in spinal cord and also in remote organs
such as kidney and bladder tissues through its antiinflammatory
and antioxidant effects.
Key words: tadalafil; spinal cord

Keywords

• tadalafil; spinal cord injury; anti-inflammatory; spinal cord, kidney; bladder.

Tadalafil attenuates spinal cord injury induced oxidative organ damage in rats

Abstract

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