Chemical Constituents, Cytotoxic, Antioxidant and Cholinesterases Inhibitory Activities of Silene Compacta (Fischer) Extracts

Abstract

In Turkey, Silene species have been used as infusion in urinary

bladder and biliary tract diseases in traditional medicine. Silene

species have been also consumed as food in Anatolia and Europe.

In this study, the phenolic, fatty acid and essential oil profiles and

the cytotoxic, antioxidant and cholinesterases inhibitory activities,

and total phenolic-flavonoid content of Silene compacta Fischer

(SC) were studied. The essential oil and fatty acid compositions

of S. compacta were determined by using GC/MS in the current

study. The chemical composition of the methanol extract was

determined using LC-MS/MS for quantitative and qualitative

purposes. The major components of the essential oil and fatty

acid were identified as α-selinene (12.4%) and palmitic acid

(26.3%), respectively. The methanol extract (SCM: S. compacta

methanol extract) which possessed the best activity in four tested

antioxidant methods among the tested extracts exhibited very

strong cholinesterases inhibitory activities. Additionaly, this

extract indicated the highest cytotoxic effect against A549 cells.

In the SCM extract, hesperidin and rutin, quinic and malic acids

were quantified by LC-MS/MS as major contituents.

Keywords

• Silene compacta,LC-MS/MS,fatty acid,essential oil,anticholinesterase,antioxidant,cytotoxicity

Chemical Constituents, Cytotoxic, Antioxidant and Cholinesterases Inhibitory Activities of Silene Compacta (Fischer) Extracts

Abstract

Silene türleri Türkiye’de halk arasında mesane ve safra yolları

infüzyonunda kullanılmaktadır.

Silene türleri Avrupa ve Anadolu’da yiyecek olarak

tüketilmektedir. Bu çalışmada Silene compacta Fischer (SC)

bitkisinin fenolik, yağ asidi ve uçucu yağ kompozisyonları

ile sitotoksik, antioksidan ve antikolinesteraz aktiviteleri,

toplam fenolik ve flavonoid içerikleri çalışılmıştır. Uçucu yağ

ve yağ asidi bileşenleri GC-MS cihazı ile belirlendi. Metanol

ekstresinin kimyasal bileşimi kalitatif ve kantitatif olarak

LC-MS/MS cihazı ile belirlendi. Uçucu yağ ve yağ asidinin ana

bileşenleri sırasıyla α-selinen (%12.4) ve palmitik asit (%26.3)

olarak belirlendi. Çalışılan dört antioksidan test yöntemlerinde

aktif olan methanol ekstresi çok güçlü antikolinesteraz enzim

inhibisyon aktivitesi göstermiştir. Aynı zamanda methanol

ekstresinin A549 hücre serilerine karşı en yüksek sitotoksik

etkiyi gösterdiği belirlenmiştir. Metanol ekstresinin LC-MS/MS

analizi sonucuna göre ana bileşenleri hesperidin, rutin, kuinik

asit ve malik asit olarak belirlenmiştir.

Keywords

• Silene compacta,LC-MS/MS,Yağ asidi,uçucu yağ,antikolinesteraz,antioksidan

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