Research Article
Chemical Constituents, Cytotoxic, Antioxidant and Cholinesterases Inhibitory Activities of Silene Compacta (Fischer) Extracts
Abstract
In Turkey, Silene species have been used as infusion in urinary
bladder and biliary tract diseases in traditional medicine. Silene
species have been also consumed as food in Anatolia and Europe.
In this study, the phenolic, fatty acid and essential oil profiles and
the cytotoxic, antioxidant and cholinesterases inhibitory activities,
and total phenolic-flavonoid content of Silene compacta Fischer
(SC) were studied. The essential oil and fatty acid compositions
of S. compacta were determined by using GC/MS in the current
study. The chemical composition of the methanol extract was
determined using LC-MS/MS for quantitative and qualitative
purposes. The major components of the essential oil and fatty
acid were identified as α-selinene (12.4%) and palmitic acid
(26.3%), respectively. The methanol extract (SCM: S. compacta
methanol extract) which possessed the best activity in four tested
antioxidant methods among the tested extracts exhibited very
strong cholinesterases inhibitory activities. Additionaly, this
extract indicated the highest cytotoxic effect against A549 cells.
In the SCM extract, hesperidin and rutin, quinic and malic acids
were quantified by LC-MS/MS as major contituents.
Keywords
Silene compacta LC-MS/MS fatty acid essential oil anticholinesterase antioxidant cytotoxicity
References
- 1. Davis PH, Mill RR, Tan K. Silene L. In: Flora of Turkey and the East Aegean Islands, suppl. 10. Edinburgh Univ. Press, Edinburgh. 1988. 2. Baytop T. Therapy with Plants in Turkey (Past and Present). Publications of Istanbul University, Istanbul.1984. 3. Laghetti G, Perrino P. Utilization of Silene vulgaris (Moench.) Garcke in Italy. Econ Bot 1994; 48: 337–9. 4. Glensk M, Wray V, Nimtz M, Schoepke T. Silenosides A-C, triterpenoid saponins from Silene vulgaris. J Nat Prod, 1999; 62: 717-21. 5. Gaidi G, Miyamoto T, Laurens V, Dubois MAL. New acylated triterpene saponins from Silene fortunei that modulate lymphocyte proliferation. J Nat Prod, 2002; 65: 1568–72. 6. Zibareva L, Yeriomina VI, Munkhjargal N, Girault JP, Dinan L, Lafont R. The phytoecdysteroid profiles of 7 species of Silene (Caryophyllaceae). Arch Insect Biochem Physiol 2009; 72: 234–48. 7. Gasiorowski K, Brokos B, Glensk M, Schöpke T. Immunomodulatory activity of the saponinrich fraction from roots of Silene vulgaris Garcke: initial study. Pharmazie 1999; 54: 864–6. 8. Mamadalieva NZ, Chenko NT, Yuldasheva NK, Egamberdieva DR, Zhanibekov A, Dzhukharova MK, Glushenkova AI. Fatty acid composition and antibacterial activity of CHCl3 extracts of three plants of the genus Silene. Chem Nat Comp 2010; 46; 95-6. 9. Kucukboyaci N, Ozçelik B, Adıguzel N, Goren AC. Fatty-acid compositions of Silene vulgaris and S. cserei subsp. aeoniopsis seeds and their antimicrobial activities. Chem Nat Compd 2010; 46: 88-91. 10. Vivek B, Savita S, Chul KS. Chemical composition and antifungal activity of essential oil and various extract of Silene armeria L. Bioresource Technol 2008; 99: 8903-8. 11. Howes MJR, Perry NSL, Houghton PJ. Plants with traditional uses and activities relevant to the management of Alzheimer’s disease and other cognitive disorders. Phytother Res 2003; 17: 1-18. 12. Ertas A, Boga M, Yılmaz MA, Yesil Y, Hasimi N, Kaya MS, Temel H, Kolak U. Chemical compositions by using LC-MS/ MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. J Agr Food Chem 2014; 62: 4601–9. 13. Yamazaki E, Inagaki M, Kurita O, Inoue T. Antioxidant activity of Japanese pepper (Zanthoxylum piperitum) fruit. Food Chem 2007; 100: 171-7. 14. Ertas A, Boga M, Yılmaz MA, Yesil Y, Tel G, Temel H, Hasimi N, Gazioglu I, Ozturk M, Ugurlu P. A detailed study on the chemical and biological profiles of essential oil and methanol extract of Thymus nummularius (Anzer tea): Rosmarinic acid. Ind Crop Prod 2015; 67: 336-45. 15. Ertas A, Boga M, Kizil M, Ceken B, Goren AC, Hasimi N, Demirci S, Topcu G, Kolak U. Chemical profile and biological activities of Veronica thymoides subsp. pseudocinerea. Pharm Biol 53; 2015: 334-9. 16. Ertas A, Yilmaz MA, Firat M. Chemical profile by LC–MS/ MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Nat Prod Res 2015; 29: 529-34. 17. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Viticult 1977; 28: 49-55. 18. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000; 71: 109-14. 19. Miller HE. A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 1971; 48: 91. 20. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181: 1199-1200. 21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26: 1231-7. 22. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agric Food Chem 2004; 52: 7970-81. 23. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95. 24. Guo N, Ding W, Wang Y, Hu Z, Wang Z, Wang Y. An LC– MS/MS method for the determination of salidroside and its metabolite p-tyrosol in rat liver tissues. Pharm Biol 2014; 52: 637–45. 25. Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S, Di Venere D. Activity of extracts from wild edible herbs against postharvest fungal diseases of fruit and vegetables. Postharvest Biol Tec 2011; 61: 72-82.
Chemical Constituents, Cytotoxic, Antioxidant and Cholinesterases Inhibitory Activities of Silene Compacta (Fischer) Extracts
Abstract
Silene türleri Türkiye’de halk arasında mesane ve safra yolları
infüzyonunda kullanılmaktadır.
Silene türleri Avrupa ve Anadolu’da yiyecek olarak
tüketilmektedir. Bu çalışmada Silene compacta Fischer (SC)
bitkisinin fenolik, yağ asidi ve uçucu yağ kompozisyonları
ile sitotoksik, antioksidan ve antikolinesteraz aktiviteleri,
toplam fenolik ve flavonoid içerikleri çalışılmıştır. Uçucu yağ
ve yağ asidi bileşenleri GC-MS cihazı ile belirlendi. Metanol
ekstresinin kimyasal bileşimi kalitatif ve kantitatif olarak
LC-MS/MS cihazı ile belirlendi. Uçucu yağ ve yağ asidinin ana
bileşenleri sırasıyla α-selinen (%12.4) ve palmitik asit (%26.3)
olarak belirlendi. Çalışılan dört antioksidan test yöntemlerinde
aktif olan methanol ekstresi çok güçlü antikolinesteraz enzim
inhibisyon aktivitesi göstermiştir. Aynı zamanda methanol
ekstresinin A549 hücre serilerine karşı en yüksek sitotoksik
etkiyi gösterdiği belirlenmiştir. Metanol ekstresinin LC-MS/MS
analizi sonucuna göre ana bileşenleri hesperidin, rutin, kuinik
asit ve malik asit olarak belirlenmiştir.
References
- 1. Davis PH, Mill RR, Tan K. Silene L. In: Flora of Turkey and the East Aegean Islands, suppl. 10. Edinburgh Univ. Press, Edinburgh. 1988. 2. Baytop T. Therapy with Plants in Turkey (Past and Present). Publications of Istanbul University, Istanbul.1984. 3. Laghetti G, Perrino P. Utilization of Silene vulgaris (Moench.) Garcke in Italy. Econ Bot 1994; 48: 337–9. 4. Glensk M, Wray V, Nimtz M, Schoepke T. Silenosides A-C, triterpenoid saponins from Silene vulgaris. J Nat Prod, 1999; 62: 717-21. 5. Gaidi G, Miyamoto T, Laurens V, Dubois MAL. New acylated triterpene saponins from Silene fortunei that modulate lymphocyte proliferation. J Nat Prod, 2002; 65: 1568–72. 6. Zibareva L, Yeriomina VI, Munkhjargal N, Girault JP, Dinan L, Lafont R. The phytoecdysteroid profiles of 7 species of Silene (Caryophyllaceae). Arch Insect Biochem Physiol 2009; 72: 234–48. 7. Gasiorowski K, Brokos B, Glensk M, Schöpke T. Immunomodulatory activity of the saponinrich fraction from roots of Silene vulgaris Garcke: initial study. Pharmazie 1999; 54: 864–6. 8. Mamadalieva NZ, Chenko NT, Yuldasheva NK, Egamberdieva DR, Zhanibekov A, Dzhukharova MK, Glushenkova AI. Fatty acid composition and antibacterial activity of CHCl3 extracts of three plants of the genus Silene. Chem Nat Comp 2010; 46; 95-6. 9. Kucukboyaci N, Ozçelik B, Adıguzel N, Goren AC. Fatty-acid compositions of Silene vulgaris and S. cserei subsp. aeoniopsis seeds and their antimicrobial activities. Chem Nat Compd 2010; 46: 88-91. 10. Vivek B, Savita S, Chul KS. Chemical composition and antifungal activity of essential oil and various extract of Silene armeria L. Bioresource Technol 2008; 99: 8903-8. 11. Howes MJR, Perry NSL, Houghton PJ. Plants with traditional uses and activities relevant to the management of Alzheimer’s disease and other cognitive disorders. Phytother Res 2003; 17: 1-18. 12. Ertas A, Boga M, Yılmaz MA, Yesil Y, Hasimi N, Kaya MS, Temel H, Kolak U. Chemical compositions by using LC-MS/ MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. J Agr Food Chem 2014; 62: 4601–9. 13. Yamazaki E, Inagaki M, Kurita O, Inoue T. Antioxidant activity of Japanese pepper (Zanthoxylum piperitum) fruit. Food Chem 2007; 100: 171-7. 14. Ertas A, Boga M, Yılmaz MA, Yesil Y, Tel G, Temel H, Hasimi N, Gazioglu I, Ozturk M, Ugurlu P. A detailed study on the chemical and biological profiles of essential oil and methanol extract of Thymus nummularius (Anzer tea): Rosmarinic acid. Ind Crop Prod 2015; 67: 336-45. 15. Ertas A, Boga M, Kizil M, Ceken B, Goren AC, Hasimi N, Demirci S, Topcu G, Kolak U. Chemical profile and biological activities of Veronica thymoides subsp. pseudocinerea. Pharm Biol 53; 2015: 334-9. 16. Ertas A, Yilmaz MA, Firat M. Chemical profile by LC–MS/ MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Nat Prod Res 2015; 29: 529-34. 17. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Viticult 1977; 28: 49-55. 18. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000; 71: 109-14. 19. Miller HE. A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 1971; 48: 91. 20. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181: 1199-1200. 21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26: 1231-7. 22. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agric Food Chem 2004; 52: 7970-81. 23. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95. 24. Guo N, Ding W, Wang Y, Hu Z, Wang Z, Wang Y. An LC– MS/MS method for the determination of salidroside and its metabolite p-tyrosol in rat liver tissues. Pharm Biol 2014; 52: 637–45. 25. Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S, Di Venere D. Activity of extracts from wild edible herbs against postharvest fungal diseases of fruit and vegetables. Postharvest Biol Tec 2011; 61: 72-82.
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Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | April 18, 2017 |
| Published in Issue | Year 2017 Volume: 21 Issue: 3 |