Tartrazine induced changes in physiological and biochemical parameters in Swiss albino mice, Mus musculus

Year 2017, Volume: 21 Issue: 3, 564 - 569, 20.06.2017

Sayema Arefin Mohammad Salim Hossain Shamme Akter Neshe Md. Mamun Or Rashid Mohammad Tohidul Amin Md. Saddam Hussain

https://doi.org/10.12991/marupj.319304

Abstract

Now-a-days synthetic food dyes are being used most

commonly as food colorant in confectionaries, drugs and

cosmetics. Present study was designed to evaluate the toxic

effect of tartrazine, a widely used azo dye, on Swiss Albino mice.

Experimental animals were treated with tested dye at a dose

level 200mg/kg & 400mg/kg body weight along with normal

diet. Various physiological and biochemical parameters were

assessed to study the toxic effect of tartrazine. Our study

revealed a highly noticeable decrease in the body weight gain

of mice at 400mg/kg dose compared with the control group. A

significant variation in the average weight of the major organs

(heart, kidney and liver) was also observed. The average

weights of the heart and kidney were increased whereas the

average weight of liver was decreased significantly. Serum

triglyceride, creatinine and bilirubin levels were significantly

increased, in contrast cholesterol level was decreased in the

groups of mice treated with tartrazine

Keywords

Azo-Dye, tartrazine, Swiss Albino Mice, Physiological changes, Serum biochemistry

Swiss albino farelerde-Mus musculus, tartrazin’in neden olduğu fizyolojik ve biyokimyasal parametrelerindeki değişiklikler

Abstract

Günümüzde sentetik gıda boyaları, şekerlemelerde, ilaçlarda ve

kozmetiklerde renklendirici olarak sıklıkla kullanılmaktadır.

Çalışmamızda, geniş kullanım alanı olan bir azo boyar maddetartrazin’in

Swiss albino farelerdeki toksik etkilerinin tespit

edilmesi hedeflendi. Normal diyetle beslenen laboratuvar

hayvanlarına tartrazin 200mg/kg ve 400mg/kg dozlarda

uygulandı. Tartrazin’in toksik etkisini incelemek için çeşitli

fizyolojik ve biyokimyasal parametreler değerlendirildi.

Çalışmamızda tartrazin’in 400 mg / kg dozunda uygulandığı

grupta yer alan farelerde kontrol grubuna kıyasla vücut ağırlığı

artışında belirgin bir düşüş olduğu tespit edildi.

Kalp, böbrek ve karaciğer gibi hayatî organların ortalama

ağırlığında önemli farklılıklar gözlendi. Kalbin ve böbreklerin

ortalama ağırlıkları artarken, karaciğerin ortalama ağırlığı

önemli derecede azaldığı tespit edildi. Tartrazin’in uygulandığı

grupta yer alan farelerde serum trigliserid, kreatinin ve bilirubin

düzeyleri anlamlı olarak artarken kolesterol düzeyinin azaldığı

tespit edildi.

Keywords

Azo boyaları, Tartrazin, Swiss Albino fare, Fizyolojik değişiklikler, Serum biyokimyası

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