Effect of Abrupt Salinity Change in the Survival of Asian Green Mussel Perna viridis (Linnaeus, 1758) Spats

Abstract

Salinity is one of the key environmental factors that affects the growth and survival of marine organisms including mussels. Five different salinity levels (40, 30, 20, 10 and 5 ppt) were used to test the effect of abrupt salinity change in the survival of hatchery-produced spats of Asian green mussel Perna viridis (Linnaeus, 1758). Spats were stocked with a density of 30 individuals per 6-L tank. Salinity manipulation was conducted after 2 days from the date of stocking. Based on the results, abrupt change and prolonged exposure to lower salinities particularly of 5 and 10 ppt (up to 5 days) are detrimental to P. viridis spats. The critical time for P. viridis spats wherein they could seclude themselves from the persistent lower salinities is 28 hours from its exposure. After which, mortality could be high at about 50% and will continue in the succeeding days if low salinity persists. Critically, no single spat can survive until the 4th day of continuous exposure to very low salinity of 5 ppt. Nevertheless, surviving individuals could still recover if salinity will return to optimum levels. Additionally, spats can readily adjust to abrupt change up to 10 ppt from the optimum salinity level as seen in the high survival in 20 and 40 ppt.

Keywords

• Perna viridis
• Salinity
• Spats
• Survival rate
• Gaping percentage

References

1. Aypa, S. M. (1990). Mussel culture. In UNDP/FAO (RAS/90/002), Regional Seafarming Development and Demonstration Project (RAS/90/002), Selected Papers on Mollusc Culture. FAO. https://www.fao.org/3/ab737e/AB737E04.htm#ch4
2. Ballesta-Artero, I., Witbaard, R., Carroll, M. L., & van der Meer, J. (2017). Environmental factors regulating gaping activity of the bivalve Arctica islandica in Northern Norway. Marine Biology, 164(5), 116. https://doi.org/10.1007/s00227-017-3144-7
3. Binzer, S. B., Lundgreen, R. B. C., Berge, T., Hansen, P. J., & Vismann, B. (2018). The blue mussel Mytilus edulis is vulnerable to the toxic dinoflagellate Karlodinium armiger- Adult filtration is inhibited and several life stages killed. PLOS ONE, 13(6), e0199306. https://doi.org/10.1371/journal.pone.0199306
4. Casila, J. C., Azhikodan, G., Yokoyama, K., Fukushima, K., & Terajima, R. (2017). Effect of rainfall on saltwater and suspended sediment dynamics in multi-branched urban tidal estuaries. JSCE Proceedings G (Environment), 73(5), 347–352.
5. Cheung, S. G. (1991). Energetics of transplanted populations of the green lipped mussel Perna viridis (Linnaeus) (Bivalvia: Mytilacea) in Hong Kong ii: growth, condition and reproduction. Asian Marine Biology, 8, 117–131.
6. Comeau, L. A., Babarro, J. M. F., Longa, A., & Padin, X. A. (2018). Valve-gaping behavior of raft-cultivated mussels in the Ría de Arousa, Spain. Aquaculture Report, 9, 68–73. https://doi.org/10.1016/j.aqrep.2017.12.005
7. de la Peña, M. R., & Franco, A. V. (2013). Culture of marine phytoplankton for aquaculture seed production. Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
8. Duncan, P. F., Andalecio, M. N., Peralta, E. M., Laureta, L. V., Hidalgo, A. N., & Napata, R. (2009). Evaluation of Production Technology, Product Quality and Market Potential for the Development of Bivalve Mollusk Aquaculture in the Philippines. Final Report FR 2009-41. Canberra, Australia: Australian Center for International Agricultural Research (ACIAR). 193p.

9. Helm, M. M., & Bourne, N. (2004). Hatchery Culture of Bivalves: A Practical Manual. In Lovatelli, A. (Ed.), FAO Fisheries Technical Paper 471. Food and Agriculture Organization of the United Nations.
10. Ingrao, D. A., Mikkelsen, P. M., & Hicks, D. W. (2001). Another introduced marine mollusk in the Gulf of Mexico: the Indo-Pacific green mussel, Perna viridis, in Tampa Bay, Florida. Journal of Shellfish Research, 20(1), 13–19.
11. Iqbal, T. H., Hajisamae, S., & Khongpuang, S. (2018). Feeding habits of Asian green mussel (Perna viridis): a case study from Andaman Sea and Gulf of Thailand. Proceedings of the Asian Academic Society International Conference, Thailand, pp. 480+484.
12. McFarland, K., Baker, S., Baker, P., Rybovich, M., &Volety, A. K. (2014). Temperature, salinity, and aerial exposure tolerance of the invasive mussel, Perna viridis, in estuarine habitats: Implications for spread and competition with native oysters, Crassostrea virginica. Estuaries and Coasts, 38(5), 1619–1628.
13. McFarland, K., Donaghy, L., & Volety, A. K. (2013). Effect of acute salinity changes on hemolymph osmolality and clearance rate of the non-native mussel, Perna viridis, and the native oyster, Crassostrea virginica, in Southwest Florida. Aquatic Invasions, 8(3), 299–310. https://doi.org/10.3391/ai.2013.8.3.06
14. Mero, F. F. C., Pedroso, F. L., Apines-Amar, M. J. S., Cadangin, J. F., Rendaje, D. C., Verde, C. S., Maquirang, J. R. H., & Piňosa, L. A. G. (2019). Influence of water management, photoperiod and aeration on growth, survival, and early spat settlement of the hatchery-reared green mussel, Perna viridis. International Aquatic Research. 11, 159–172. https://doi.org/10.1007/s40071-019-0226-9
15. Nicastro, K. R., Zardi, G. I., McQuaid, C. D., Stephens, L., Radloff, S., & Blatch, G. L. (2010). The role of gaping behaviour in habitat partitioning between coexisting intertidal mussels. BioMed Central Ecology, 10, 17. https://doi.org/10.1186/1472-6785-10-17
16. Philippines Statistics Authority (PSA). (2019). Fisheries Statistics of the Philippines 2016–2018, Volume 27. Quezon City: Philippines, Philippine Statistics Authority. 302p. https://psa.gov.ph/content/fisheries-statistics-philippines
17. Ponce-Palafox, J., Martinez-Palacios, C. A., & Ross, L. G. (1997). The effects of salinity and temperature on the growth and survival rates of juvenile white shrimp, Penaeus vannamei, Boone, 1931. Aquaculture, 157, 107–115. https://doi.org/10.1016/S0044-8486%2897%2900148-8
18. Rajagopal, S., Venugopalan, V. P., van der Velde, G., & Jenner, H. A. (2006). Greening of the coasts: a review of the Perna viridis success story. Aquatic Ecology, 40(3), 273–297. https://doi.org/10.1007/s10452-006-9032-8 Rowchai, S. (2004 abstract only). Effects of low salinity on mortality of green-lipped mussel (Perna viridis Linneaus). https://agris.fao.org/agris-search/search.do?recordID=TH2005001692
19. Segnini de Bravo, M. I. (2003). Influence of salinity on the physiological conditions in mussels, Perna perna and Perna viridis (Bivalvia: Mytilidae). Revista de Biologia Tropical, 51(4), 153–158.
20. Segnini de Bravo, M. I., Chung, K. S., & Pérez, J. E. (1998). Salinity and temperature tolerances of the green and brown mussels, Perna viridis and Perna perna (Bivalvia: Mytilidae). Revista de Biologica Tropical, 46(5), 121-125.
21. Soon, T. K., & Ransangan, J. (2016). Feasibility of green mussel, Perna viridis farming in Marudu Bay, Malaysia. Aquaculture Reports, 4, 130–135. https://doi.org/10.1016/j.aqrep.2016.06.006
22. Su, Y., Ma, S., & Feng, C. (2010). Effects of salinity fluctuation on the growth and energy budget of juvenile Litopenaeus vannamei at different temperatures. Journal of Crustacean Biology, 30(3), 430–434. https://doi.org/10.1651/09-3269.1
23. Taib, A. M., Madin, J., & Ransangan, J. (2016). Density, recruitment and growth performance of Asian green mussel (Perna viridis) in Marudu Bay, Northeast Malaysian Borneo, three years after a massive mortality event. Songklanakarin Journal of Science and Technology, 38(6), 631–639.
24. Vu, N., & Huynh, T. (2020). Optimized live feed regime significantly improves growth performance and survival rate for early life history stages of Pangasius Catfish (Pangasianodon hypophthalmus). Fishes, 5(3), 20. https://doi.org/10.3390/fishes5030020
25. Wang, Y., Hu, H., Cheung, S. G., Shin, P. K. S., Lu, W., & Li, J. (2012). Immune parameter changes of hemocytes in green-lipped mussel Perna viridis exposure to hypoxia and hyposalinity. Aquaculture, 356-357, 22-29. https://doi.org/10.1016/j.aquaculture.2012.06.001
26. Wang, Y., Hu, M., Wong, W. H., Cheung, S. G., & Shin, P. K. S. (2011). Combined effects of dissolved oxygen and salinity on growth and body composition of juvenile green-lipped mussel Perna viridis. Journal of Shellfish Research, 30(3), 851-857. https://doi.org/10.2983/035.030.0326
27. Wu, F., Xie, Z., Lan, Y., Dupont, S., Sun, M., Cui, S., Huang, X., Huang, W., Liu, L., Hu, M., Lu, W., & Wang, Y. (2018). Short-term exposure of Mytilus coruscus to decreased pH and salinity change impacts immune parameters of their haemocytes. Frontiers in Physiology, 9, 166. https://doi.org/10.3389/fphys.2018.00166
28. Yuan, W. S., Walters, L. J., Brodsky, S. A., Schneider, K. R., & Hoffman, E. A. (2016). Synergistic effects of salinity and temperature on the survival of two nonnative bivalve molluscs, Perna viridis (Linnaeus 1758) and Mytella charruana (d’Orbigny 1846). Journal of Marine Sciences, 2016, 9261309. https://doi.org/10.1155/2016/9261309