Evaluation Of The Effect Of Autologous Dermis Graft On Rat Achilles Tendon Healing

Year 2025, Volume: 9 Issue: 1, 19 - 28, 01.04.2025

Muhammet Doğan , Mehmet Mesut İnan , İkram Esen , Mehmet Bayram , Serkan Erbatur

Abstract

Aim: The Achilles tendon is one of the most frequently injured tendons. It is more common in young and middle-aged individuals who are particularly active in sports. Re-surgery or permanent disabilities may occur as a result of tendon rupture after tendon repair. Various methods to enhance Achilles tendon healing have been described in the literature. However, there is limited data on the use of dermis grafts for tendon augmentation. Dermis grafts; Since they are core tissue, they have less foreign body reaction, can be obtained in a short time, and can be an alternative due to less donor area morbidity. The aim of our study is to reduce the risk of re-rupture by strengthening tendon healing in Achilles tendon injuries and to determine the effectiveness of autologous dermis grafts on healing for strong healing.

Methods: In our study, 20 male rats were randomly divided into two groups as Control and Experimental groups. All rats underwent tendon repair after an atraumatic, full-thickness, straight incision was applied to the right Achilles tendon. After tendon repair in the rats in the experimental group; dermis grafts taken from the back were wrapped around the tendon repair area and sutured. The other rats constituted the control group. Rats were sacrificed at week 4 and their Achilles tendons were removed and examined histopathologically, immunohistochemically and biomechanically.

Results: Tendons were intact and no rupture was observed in all samples. Positive results were observed in favor of the experimental group in type I collagen and type I/type III collagen density and biomechanical examinations in rats repaired with dermis grafts.

Conclusion: As a result of our study; it was shown that dermis grafts can positively contribute to tendon healing. Tendon repair with dermis grafts can be a good alternative to other methods for tendon augmentation or strengthening.

Keywords

Achilles tendon, Tendon healing, Dermis graft

Project Number

2022-41

Otolog Dermis Greftinin Rat Aşil Tendonu İyileşmesi Üzerindeki Etkisinin Değerledirilmesi

Abstract

Amaç: Aşil tendon sık yaralanan tendonlardan, özellikle aktif spor uğraşı olan genç-orta yaşta bireylerde daha sık görülmektedir. Tendon tamiri sonrası tendonun tekrar kopması sonucunda tekrar ameliyat veya kalıcı sakatlıklar gelişebilmektedir. Literatürde aşil tendon iyileşmesini artrıcı çeşitli yöntemler tarif edilmiştir. Ancak, dermis greftin tendon augmentasyonu amacıyla kullanımına dair kısıtlı veri mevcuttur. Dermis greftleri; öz doku olduğundan yabancı cisim reaksiyonu az, kısa zamanda alınabilen ve daha az donör alan morbiditesi nedeniyle alternatif olabilecek niteliktedir. Çalışmamızdaki amaç, aşil tendon yaralanmalarında tendon iyileştirmesini güçlendirirerek tekrar kopma riskini azaltmak ve güçlü iyileşme için otolog dermis greftinin iyileşme üzerinde etkinliğini belirlemektir.

Yöntem: Çalışmamızda 20 erkek rat Kontrol ve Deney grubu olarak rastgele iki gruba ayrıldı. Tüm ratların sağ aşil tendonuna atravmatik, tam kat, düz kesi uygulandıktan tendon onarımı yapıldı. Deney grubundaki ratlara tendon onarımı sonrasında; sırttan alınan dermis grefti tendon onarım bölgesine sarılarak sütüre edildi. Diğer ratlar kontrol grubunu oluşturdu. Ratlar 4. haftada sakrifiye edilerek aşil tendonları cikartılarak histopatolojik, immünohistokimyasal ve biyomekanik olarak incelendi.

Bulgular: Bütün numunelerde tendon intakt ve rüptür gözlenmedi. Dermis grefti ile onarım yapılan ratlarda tip I kollajen ve tip I/tip III kollajen yoğunluğunda ve biyomekanik incelemelerde deney grubu lehine olumlu sonuçlar izlendi.

Sonuç: Çalışmamız sonucunda; dermis greftinin tendon iyileşmesine olumlu katkı sağlayabileceğini gösterdi. Dermis grefti ile tendon onarımı; tendon augmentasyonu veya güçlendirmesi için diğer yöntemlere göre iyi bir alternatif olabilir.

Keywords

Aşil tendon, Tendon iyileşmesi, Dermis grefti

Project Number

2022-41

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