Investigation of Exopolysaccharide Production Capacities of Cyanobacterial Strains Isolated from Lake Uluabat, Turkey

Abstract

Exopolysaccharides (EPS), important metabolites of microalgae and cyanobacteria, can be used as food additives, drug active substances, and in detergents, adhesives, and waste water treatment processes. Cyanobacterial EPSs are divided into two groups: the first group includes EPSs which are associated with the cell membrane (capsular, sheath), while the second group EPSs are released into the culture medium. The capsular polysaccharides can be found in the form of a sheath, capsule or mucilage. Cyanobacterial EPSs are heteropolymer structures consisting of monosaccharides, lipids, proteins and DNA. In this study, chemical characterization and metal binding properties of 3 different cyanobacterial (Cyanobium sp., Anabaena sp. and Limnothrix sp.) EPSs were investigated. Total protein concentrations ranged between 98,8 mg g-1 and 171,6 mg g-1 and total carbohydrate concentrations ranged between 252,1 mg g-1 and 320,5 mg g-1 per dry weight of the cyanobacterial EPS prepared in the study. Elemental compositions and metal (Cu+2, Cr+6 and Ni+2) adsorption properties of EPSs were determined with SEM-EDS analysis. According to elemental analysis EPS molecules mainly contained carbon (35,63-65,12%) and oxygen (21,39-40,27%).

Keywords

• Lake Uluabat,cyanobacterial exopolysaccharide,protein,carbohydrate,metal adsorption

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