Emiliana huxleyi (Haptophyta)’nin Büyümesinde İki Farklı Fe konsantrasyonu (10nM ve 100nM) ve EDTA (Etilendiamintetraasetat)’nın Etkisi.

Abstract

Biyolojik olaylarda önemli görevleri olan demir (Fe)’in sucul sistemde alınabilir formlarını ve alımını etkileyen faktörler halen tam olarak bilinmemektedir. Çalışmada, iki farklı demir (10-100 nM) içeren F/2 besi ortamında, suni ligand EDTA (Ethylenediaminetetraacetate) (100µM)’nın, Emiliana huxleyi’nin büyümesine etkileri araştırılmıştır. Denemede ortam sıcaklığı 18 ± 2 °C ve aydınlanma şiddeti ise 80µmol m-2 s-1 olacak şekilde ayarlanmıştır. Denemede kullanılan deniz suyu, makro besleyiciler ve EDTA, Chelex-100 reçinesi ile eser metallerinden arındırılmıştır. Elde edilen veriler değerlendirildiğinde, en yüksek hücre yoğunluğu, klorofil-a miktarı ve hücresel Fe oranının 100nM Fe ve EDTA içeren E.huxleyi kültürlerine ait olduğu, en yüksek spesifik büyüme hızının ise 10nM Fe+EDTA içeren kültürlerden elde edildiği saptanmıştır.

Keywords

• E.huxleyi
• büyüme
• alınabilir demir
• EDTA

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