Emiliana huxleyi (Haptophyta)’nin Büyümesinde İki Farklı Fe konsantrasyonu (10nM ve 100nM) ve EDTA (Etilendiamintetraasetat)’nın Etkisi.

Year 2021, Volume: 4 Issue: 1, 11 - 18, 30.01.2021

Selin Sayın , Oya Işık

Abstract

Biyolojik olaylarda önemli görevleri olan demir (Fe)’in sucul sistemde alınabilir formlarını ve alımını etkileyen faktörler halen tam olarak bilinmemektedir. Çalışmada, iki farklı demir (10-100 nM) içeren F/2 besi ortamında, suni ligand EDTA (Ethylenediaminetetraacetate) (100µM)’nın, Emiliana huxleyi’nin büyümesine etkileri araştırılmıştır. Denemede ortam sıcaklığı 18 ± 2 °C ve aydınlanma şiddeti ise 80µmol m-2 s-1 olacak şekilde ayarlanmıştır. Denemede kullanılan deniz suyu, makro besleyiciler ve EDTA, Chelex-100 reçinesi ile eser metallerinden arındırılmıştır. Elde edilen veriler değerlendirildiğinde, en yüksek hücre yoğunluğu, klorofil-a miktarı ve hücresel Fe oranının 100nM Fe ve EDTA içeren E.huxleyi kültürlerine ait olduğu, en yüksek spesifik büyüme hızının ise 10nM Fe+EDTA içeren kültürlerden elde edildiği saptanmıştır.

Keywords

E.huxleyi, büyüme, alınabilir demir, EDTA

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