Emiliana huxleyi (Haptophyta)’nin Büyümesinde İki Farklı Fe konsantrasyonu (10nM ve 100nM) ve EDTA (Etilendiamintetraasetat)’nın Etkisi.

Öz

Biyolojik olaylarda önemli görevleri olan demir (Fe)’in sucul sistemde alınabilir formlarını ve alımını etkileyen faktörler halen tam olarak bilinmemektedir. Çalışmada, iki farklı demir (10-100 nM) içeren F/2 besi ortamında, suni ligand EDTA (Ethylenediaminetetraacetate) (100µM)’nın, Emiliana huxleyi’nin büyümesine etkileri araştırılmıştır. Denemede ortam sıcaklığı 18 ± 2 °C ve aydınlanma şiddeti ise 80µmol m-2 s-1 olacak şekilde ayarlanmıştır. Denemede kullanılan deniz suyu, makro besleyiciler ve EDTA, Chelex-100 reçinesi ile eser metallerinden arındırılmıştır. Elde edilen veriler değerlendirildiğinde, en yüksek hücre yoğunluğu, klorofil-a miktarı ve hücresel Fe oranının 100nM Fe ve EDTA içeren E.huxleyi kültürlerine ait olduğu, en yüksek spesifik büyüme hızının ise 10nM Fe+EDTA içeren kültürlerden elde edildiği saptanmıştır.

Anahtar Kelimeler

• E.huxleyi
• büyüme
• alınabilir demir
• EDTA

Kaynakça

1. Brand, L.E., Sunda, W.G., Guillard, R.R.L. (1983). Limitation of marine phytoplankton reproductive rates by zinc, manganese, and iron. Limnol. Oceanogr., 28: 1182-1198.
2. Brand, L.E., Sunda, W.G., Guillard, R.R.L. (1986). Reduction of marine Phytoplankton Reproduction rates by Copper and Cadmium. J.Exp.Mar. Biol. Ecol., 96: 225-250.
3. Boyd, C.E. (1979). Water Quality in Warmwater Fish Ponds. Auburn University Agricultural Experiment Station.
4. Martin, J.H., Fitzwater, S.E. (1988). Iron deficiency limits phytoplankton growth in the north-east Pacific subarctic. Nature, 331:341-343.
5. Archer, D., Winguth, A., Lea, D., Mahowald, N. (2000). What caused the glacial/interglacial atmospheric pCO2 cycles? Reviews of Geophysics, 38:159-189.
6. Braarud, T. (1962). Species distribution in marine phytoplankton. Journal of the Oceanographical Society of Japan 20th Anniversary. Volume:628-649.
7. van den Hoek, C., Mann, D.G., Jahs, H.M. (1995). Algae an Introduction to phycology. Cambridge University Press.133, 219:614.
8. Geringa, L.J.A., de Baar, H.J.W., Timmermans. (2000). A comparison of iron limitation of phytoplankton in natural aceanic waters and laboratory media conditioned with EDTA. Marine Chemistry. 68; 335-346.

9. Fernandez, E., Maranon, E., Harbour, D.S., Kristiansen, S., Heimdal, B.R. (1996). Patterns of carbon and nitrogen uptake during blooms of Emiliania huxleyi in two Norweigian fjords. Journal of Plankton Research, 18: 2349-2366.
10. Guillard, R.R.L. (1973). Division Rates in Handbook of Phycological methods (J. R. ETEIN editör). Culture Methods and Growth Measurements, Chambridge Universty Press, Chambridge, pp.289-311.
11. Martin, J.L., Gordan, R.M., Fitzwater, S.E., Broenkow, W.W. (1989). Deep-Sea Research. Part A. Oceanographic Research, Page:36
12. Öztürk, M., Steinnes, E., Sakshaug, E. (2002). Iron speciation in the Trondheim Fjord from the perspective of iron limitation for phytoplankton. Estuarine, Coastal and Shelf Sciences, 55:197-212
13. Öztürk, M., Vadstein, O., Sakshaug, E. (2003). The effects of enhanced phytoplankton production on iron speciation and removal in mesocosm experiments in a land-locked basin of Hopavagen, Norway. Marine Chemistry, 84(1-2): 3–17.
14. Öztürk, M., Bizsel, N., Steinnes, E. (2003). Iron speciation in eutrophic and oligotrophic Mediterranean coastal waters. Impact of phytoplankton and protozoan blooms on iron distribution. Marine Chemistry 81, 19–36.
15. Paasche, E. (2002). A Review of The Coccolithophorid Emiliania huxleyi (Prymnesiophyceae) with Particular Reference to Growth, Coccolith formation, and Calsification – Photosynthesis İnteractions. Phyqologia (2002) Volume 40(6):503-529.
16. Saydam, A.C., Polat, I. 1999. The impact of Saharan dust on the occurrence of algae bloom. Proceeding of EUROTRAC Symposium 98. Pp.656-663 Eds: Borrell, P. M and Borrell, P. WITpress. Southampton.
17. Schoemann, V., de Baar H.J.W., de Jong, J.T.M., LANCELOT, C. (1998). Effects of phytoplankton blooms on the cycling of manganese and iron in coastal waters. Limnol. Oceanogr. 43:1427–1441.
18. Shkolnik, M.Y. (1984). Trace Elements in Plants. Develop. Crop Sci. V. 6. Elsevier.
19. Sunda, W.G. (1989). Trace Metals Interactins with Marine Phytoplankton. Biol. Oceonogr., 6:411-442.
20. Sunda, W.G., Huntsman, S.A. (1995). Iron uptake and growth limitation in oceanic and coastal phytoplankton. Mar. Chem., 50: 189-206.
21. Wells, M.L ., Zorkin, N.G., Lewis, A.G. (1983). Bioavailability and Bioaccumulation of Iron in the Sea (W. G. SUNDA editör). The Biogeochemistry of Iron in Seawater, John Wiley & Sons Ltd, Baffins Lane, Chichester, West Sussex PO19 1 UD, England, 41-85.