Türkiye’de yetiştirilen beş sığır ırkında MYF5 gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi

Abstract

Bu çalışmada Türkiye’de yetiştirilen Simental (SİM, n= 75), Holstein (HL, n=100), İsviçre Esmeri (ES, n=75), Doğu Anadolu Kırmızısı (DAK, n= 40) ve Boz Irk (BI, n=40) sığırlarının MYF5 gen polimorfizminin restiriksiyon parçacık uzunluk polimorfizmi (RFLP) metoduyla belirlenmesi amaçlanmıştır. MYF5 allelle ve genotipleri elde edilen polimeraz zincir reaksiyonu (PCR) ürünlerine TaqI kesim enzimi ile muamele edilmesiyle belirlenmiştir. Çalışmada incelenen ırklar içinde en yüksek AA genotip frekansı DAK (0.20), en yüksek GG genotip frekansı BI (0.53) ve en yüksek AG genotip frekansı ise ES (0.53) ırkında görülmüştür. MYF5 geni yönünden incelenen ırklar arasında en yüksek A allel frekansı DAK ırkında (0.43), en yüksek G allelinin frekansı ise BI’ta (0.73) bulunmuştur. Çalışma sonunda incelenen beş sığır ırkının da MYF5 geni yönünden Hardy-Weinberg dengesinde olduğu görülmüştür.

Keywords

• Sığır,Genetik belirteç,MYF5,Polimorfizm

Detection of MYF5 gene polymorphism with PCR-RFLP method in five cattle breeds breeding in Turkey

Abstract

The aim of this study was to detection of the MYF5 gene polymorphism in Simmental (SIM, n=75), Holstein (HL, n=100), Brown Swiss (ES, n=75), East Anatolian Red (EAR, n=40) and Turkish Grey (TG, n=40) cattle breeds raised in Turkey through restriction fragment length polymorphism (RFLP) method. The MYF5 alleles and genotypes were detected by digesting the polymerase chain reaction (PCR) products with TaqI endonuclease enzyme. Among the breeds examined for MYF5 gene, the highest AA genotype frequency was found in the EAR cattle breed (0.20); the highest GG genotype frequency was found in the TG cattle breed (0.53) and the highest AG genotype frequency was found in the ES cattle breed (0.53). Among the breeds examined, the highest A allele frequency was found in the EAR cattle breed (0.43) whereas the highest G allele frequency was found in the TG cattle breed (0.73). At the end of the study, it was found that examined five cattle breeds were in Hardy-Weinberg equilibrium (HWE) in terms of MYF5 gene.

Keywords

• Cattle,Genetic marker,MYF5,Polymorphism

References

1. Bhuiyan MSA, Kim NK, Cho YM, Yoon D, Kim KS, Jeon JT, Lee JH (2009) Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle. Livestock Science 126: 292-297.
2. Chung ER, Kim WT (2005) Association of SNP marker in IGF-I and MYF5 candidate genes with growth traits in Korean cattle. Asian-Australasian Journal of Animal Sciences 18: 1061-1065.
3. Ertuğrul M, Dellal G, Elmacı C, Akın AO, Pehlivan E, Soysal Mİ, Arat S (2010) Çiftlik hayvanları genetik kaynaklarının korunması ve sürdürülebilir kullanımı. VII. Türkiye Ziraat Mühendisliği Kongresi Ankara, s. 179-198.
4. Kišacová J, Kúbek A, Meluš V, Čanakyová Z, Řehout V (2009) Genetic polymorphism of MYF-5 and myostatin in Charolais breed. Journal of Agrobiology 26 (1): 7-11.
5. Kovács K, Völgyi Csík J, Zsolnai A, Györkös I, Fésüs L (2006) Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Archives Animal Breeding 49(3): 236-249.
6. Li C, Basarab J, Snelling WM, Benkel B, Murdoch B, Moore SS (2002) The identification of common haplotypes on bovine chromosome 5 within commercial lines of Bos taurus and their associations with growth traits. Journal of Animal Science 80: 1187-1194.
7. Li C, Basarab J, Snelling WM, Benkel B, Murdoch B, Hansen C, Moore SS (2004) Assessment of positional candidate genes MYF5 and IGF-I for growth on bovine chromosome 5 in commercial lines of Bos taurus. Journal of Animal Science 82: 1-7.
8. Maak S, Neumann K, Swalve H (2006) Identification and analysis of putative regulatory sequences for the MYF5/MYF6 locus in different vertebrate species. Gene 379: 141-147.

9. Nei M (1987) Molecular Evolutionary Genetics. Columbia University Press, New York.
10. Robakowska-Hyzorek D, Oprzadek J, Zelazowska B, Olbromski R, Zwierzchowski L (2010) Effect of the g.–723G → T polymorphism in the bovine myogenic factor 5 (Myf5) gene promoter region on gene transcript level in the longissimus dorsi muscle and on meat traits of polish Holstein-Friesian cattle. Biochemical Genetics 48: 450-464.
11. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Vol. 3, 2nd Edition., Cold Spring Harbor Laboratory, Cold-Spring Harbor, New York.
12. Seong J, Don Oh J, Cheong C, Lee KW, Lee HK, Suh DS, Gwang JJ, Do Park K, Kong HS (2011) Association between polymorphisms of Myf5 and POU1F1 genes with growth and carcass traits in Hanwoo (Korean cattle). Genes & Genomics 33: 425-430.
13. Ujan JA, Zan LS, Ujan SA, Wang HB (2011a) Association between polymorphism of MyF-5 gene with meat quality traits in indigenous Chinese cattle breeds. International Conference on Asia Agriculture and Animal, Hong Kong, p. 50-55.
14. Ujan JJ, Zan LS, Wang HB, Ujan SA (2011b) The effect of myogenic factor 5 polymorphism on the meat quality in Chinese Bos taurus. Agriculturae Conspectus Scientificus 76(4): 373-377.
15. Unanian MM, Barreto CC, Cordeiro CMT, Freitas AR, Josahkian LA (2002) Possible associations between bovine growth hormone gene polymorphism and reproductive traits. Brazilian Archives of Biology and Technology 45(3): 293-299.
16. Yeh F, Yang RC, Boyle T (2015) Popgene (v.1.32), Microsoft Windows-based freeware for population genetic analysis, 2000. http://www.ualberta.ca/~fyeh/Pop32.exe, Accessed: 31 August 2015.