Salvia candidissima Vahl.’ın uçucu yağ ve ekstraktının kimyasal bileşenlerinin, antioksidan ve antifungal aktivitesinin belirlenmesi

Abstract

Bu çalışmanın amacı, ada çayı (Salvia candidissima Vahl.) bitki uçucu yağının bileşenlerinin ve bitki patojeni fungal etmenlere karşı antifungal etkinlikleri, bitki metanolik ekstraktının ise antioksidan aktivitelerinin araştırılmasıdır. S. candidissima uçucu yağında GC/MS kullanılarak 54 bileşen belirlenmiştir. Tanımlanan bu bileşenler uçucu yağın % 99.98’ini oluşturduğu saptanmıştır. Uçucu yağın temel bileşenleri Spathulenol (% 12,75), Caryophyllene oxide (% 8.67), Ledene oxide (% 6.98) ve o-Cymene (% 6.03) olarak belirlenmiştir. Uçucu yağın, antifungal etkinliği fungal hastalık etmenlerinden Rhizoctonia solani ve Aternaria solani’ye karşı araştırılmıştır. Çalışma sonucunda 10 μl petri^-1 uçucu yağ uygulaması sonucunda A. solani’nin miselyum gelişimini % 57.92 oranında R. solani’nin miselyum gelişimini ise % 51.87 oranında engellediği belirlenmiştir. Bitki ekstraktları ile yapılan antioksidan çalışması sonucunda, Serbest Radikal Giderme DPPH (lC₅₀ 22.96 ± 0.45), Demir İndirgeme Gücü (FRAP 1.20 ± 0.16 mmol TE g^-1 eksrakt), Bakır İndirgeme Gücü (CUPRAC 3.30 ± 0.12mmol TE g^-1 ekstrakt) ve Serbest Katyon Radikali Giderme TEAC ise (lC₅₀ 9.25 ± 0.40 (µg ml^-1)) olarak belirlenmiştir. Toplam Fenolik (TP) ve Toplam Flavonoid (TF) içeriği sırasıyla 83.53 ± 5.92 mg GAE g^-1 ekstrakt ve 59.02 ± 3.59 mg QE g^-1 ekstrakt olarak tespit edilmiştir. Bu sonuçlar; S. candidissima uçucu yağın bitki patojeni funguslara karşı dikkate değer bir antifungal aktiviteye sahip olmasının yanısıra bitki metanolik ekstraktları güçlü bir antioksidant kapasitesine sahip olduğunuda göstermiştir.

Keywords

• Antioksidant,Antifungal,Uçucu yağ,Kimyasal bileşen,Salvia candidissima Vahl.

Determination of the chemical components, antioxidant and antifungal activities of essential oil and plant extract of Salvia candidissima Vahl.

Abstract

The aim of this study is to investigate the chemical components, antioxidant and antifungal activities of the essential oil and plant methanolic extract of Salvia candidissima Vahl. plant, which is one of the sage species. By using Gas Chromatography (GC) and Gas Chromatography Mass Spectrometry (GC-MS) method, it was determined that there are 54 components in the essential oil of S. candidissima. These identified components were found to constitute 99.98% of the essential oil. The main components of the essential oil were determined as Spathulenol (12.75%), Caryophyllene oxide (8.67%), Ledene oxide (6.98%) and o-Cymene (6.03%). A antifungal activity of the essential oil against Rhizoctonia solani and Aternaria solani was found. As a result of the study, it was determined that the mycelial growth of A. solani was inhibited by 57.92% as a result of application of 10 μL petri-¹ essential oil, while that of R. solani was inhibited by 51.87%. As a result of the antioxidant study conducted with plant extract, the values of Free Radical Scavenging DPPH (lC₅₀ 22.96 ± 0.45), Iron Reduction Power (FRAP 1.20 ± 0.16 mmol TE g^-1 extract), Copper Reduction Power (CUPRAC 3.30 ± 0.12 mmol TE g^-1 extract) and Free Cation Radical Scavenging TEAC (lC₅₀ 9.25 ± 0.40 (µg ml^-1)) were determined. The Total Phenolic (TP) and Total Flavonoid (TF) contents were found as 83.53 ± 5.92 mg GAE g^-1 extract and 59.02 ± 3.59 mg QE g^-1 extract, respectively. These results showed that the essential oil and plant methanolic extract of S.candidissima has a significant antifungal activity against plant pathogenic fungi and strong antioxidant activity.

Keywords

• Antioxidant,Antifungal,Essential oil,Chemical component,Salvia candidissima Vahl.

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