In vitro and in vivo assessment of the sensitivity of some tangerine mutants to Alternaria alternata pv. citri

Abstract

Alternaria brown spot disease caused by the Alternaria alternata pv. citri factor affects the leaves, branches and fruits of many types of tangerines and their hybrids. This disease has caused serious declines in the plantation areas of Minneola tangelo which is widely produced in the Mediterranean Region while at the same time it has hindered the expansion of the area of cultivation of late maturing tangerines like Fortune. The isolate used in this study was obtained from a commercial orchard of ‘Minneola tangelo’ in the province of Adana, Turkey. The sequence analysis of the application of this isolates shows that a matchup of 99% was achieved with isolate AHS-467.6 on the scale of the IFS primer. The aim of the study was to identify tolerant genotypes from the mutants bred form the ‘Fortune’ tangerine which is known to be sensitive to the brown spot disease of citrus. The sensitivity of individual mutants was tested by using leaves under in vitro conditions. The disease was inoculated to tolerant mutant varieties under in vivo conditions and the results registered. The results of the study showed that 9 mutant varieties among the 110 individuals under study were found to be tolerant against the disease.

Keywords

• Citrus,Mutation,Alternaria brown spot disease,Fortune,Mandarin

In vitro ve in vivo koşullarında bazı mandarin mutantlarının Alternaria alternata pv. citri etmenine duyarlılıklarının değerlendirilmesi

Abstract

Alternaria alternata pv. citri etmeninin neden olduğu Alternaria kahverengi leke hastalığı birçok mandarin ve melezlerinin yaprak, dal ve meyvelerini etkiler. Söz konusu hastalık Akdeniz bölgesinde yetiştiriciliği yapılan Minneola tanjelonun dikim alanlarında ciddi azalmalara neden olmuş ve ayrıca yetiştiricilik açısından geççi bir çeşit olan Fortune gibi mandarin çeşitlerinin gelişimini engellemiştir. Çalışmada kullanılan izolat Adana ilinde bulunan ticari ‘Minneola tanjelo’ bahçelerinden elde edilmiştir. Bu izolat sekans analizi sonucunda ITS1 primerine göre %99 oranında AHS-467-6 izolatı ile eşleşme sağlamıştır. Mutasyon ıslahı ile turunçgillerde kahverengi leke hastalığına hassas olduğu bilinen ‘Fortune’ mandarini mutantları arasından tolerant genotiplerin elde edilmesi hedeflenmiştir. In vitro koşullarda koparılmış yapraklar kullanılarak mutant bireylerin hastalığa karşı hassasiyetleri belirlenmiştir. Ayrıca, tolerant olarak bulunan mutantların fidanlarına in vivo koşullarda hastalık inokulasyonu yapılarak sonuçlar kontrol edilmiştir. Çalışma sonucunda 111 mutant birey arasından 9 adet mutant birey hastalığa karşı tolerant olarak bulunmuştur.

Keywords

• Turunçgil,Fortune,Mutasyon,Alternaria kahverengi leke hastalığı,Mandarin

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