Türkiye’den toplanan yerel soğan çeşitlerinin soğan sak nematodu Ditylenchus dipsaci’ye dayanıklılık reaksiyonları

Abstract

Türkiye’ den toplanmış olan toplam olarak 27 farklı yerel soğan çeşidinin soğan sak nematoduna dayanıklılıkları taranmıştır. Çalışma 20°C’ de %70 nem koşullarında 16/8 saat (aydınlık/ karanlık) periyodunda büyütme dolabında gerçekleştirilmiştir. Bitkiler 7 X 8 cm boyutlarında plastik saksılarda %45 kum, %45 killi milli toprak, %10 oranında organik madde karışımında yetiştirilmiştir. İki yüz nematod içeren 10µl nematod süspansiyonu 3-4 yaprak aşamasında bitkilere bulaştırılmıştır. Bitkiler nematod inokulasyonundan 6 hafta sonra hasat edilmiş ve bitkilerdeki nematodlar sayılmıştır. Düşük nematod üremesi görülen bitki çeşitleri ikinci kez Ditylenchus dipsaci ile inokule edilmiştir. Bitki çeşitlerinde nematod üremesine göre hassas standart çeşit Betopanko ile karşılaştırmalı olarak orta dayanıklıdan yüksek oranda hassas reaksiyona kadar gruplandırılmıştır. Otuz numaralı çeşit iki denemede de en düşük nematod üremesi göstermiştir ve birinci denemede orta dayanıklı, ikinci denemede ise orta hassas olarak gruplandırılmıştır. Yirmi üç ve 25 numaralı çeşitler hassas kontrole göre düşük sayıda nematod üremesine sahip olmuş ve nihai olarak orta hassas olarak gruplandırılmıştır.

Keywords

• nematod üremesi
• Allium cepa
• Bitki paraziti nematod
• Büyütme dolabı testlemesi
• Dayanıklılık

Project Number

Proje No: 111O222

Resistance reactions of onion landraces collected from Turkey to the stem and bulb nematode Ditylenchus dipsaci

Abstract

A total of 27 accessions of different onion landraces from Turkey were screened for their resistance to stem and bulb nematode. The study was carried out in a growth chamber at 20°C, with a 16/8 h (light/dark) photoperiod and at 70% relative humidity. The plants were grown in 7 X 8 cm diameter plastic pots filled with a mixture of 45% sand, 45% clay loam soil, 10% organic matter. Two hundred nematodes in 10 µl nematode suspension were inoculated to each plant at the 3-4 leaves stage. Plants were harvested six weeks after inoculation and number of nematodes was counted. Onion landraces that had low nematode reproduction were subjected to a second screening test. The landraces were classified from moderately resistant to highly susceptible according to their nematode reproduction in comparison to susceptible standard cultivar Betapanko. Accession 30 had the lowest number of nematodes in the both experiments and was classified moderately resistant and moderately susceptible in the first and second experiments, respectively. The accessions 23 and 25 had lower number of nematodes relative to standard cultivar and were classified as moderately susceptible in conclusion.

Keywords

• Allium cepa
• Growth chamber testing
• Nematode reproduction
• Plant parasitic nematode
• Resistance

Supporting Institution

TÜBİTAK

Project Number

Proje No: 111O222

Thanks

This study was supported by Turkish Scientific and Technical Research Council (Project number: 111O222). Onion materials for the study provided by Pamukkale University Plant Genetics and Agricultural Biotechnology Application and Research Center (Denizli, Turkey), the Turkish Ministry of Agriculture and Forestry, Aegean Agricultural Research Institute, National Gene Bank (İzmir, Turkey) and Atatürk Horticultural Central Research Institute (Yalova, Turkey).

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