Şeker sorgumda [Sorgum bicolor (L.) Moench] bitki boyunun SSR marker analizi

Abstract

Sorgum (Sorghum bicolor (L.) Moench), düşük girdi ile yüksek biyokütle üretebilme yeteneğine sahip umut verici biyo-enerji bitkilerinden biridir. Biyoetanol üretiminde kayda değer katkıları olan bitki boyu, biyokütle verimini oluşturan en önemli bileşenler arasındadır. Bu çalışmada, sorgum genotipleri bitki boyu QTL'leri ile ilişkili dört SSR marker ile taranmıştır. Moleküler analizler, ardışık 2 yıl ve 2 farklı ortamda doğrulanmıştır. Çalışmanın ilk yılında 551 genotipten oluşan sorgum koleksiyonunda moleküler analizler ve tarla koşullarında bitki boyu ölçümleri yapılmıştır. İkinci yılda ise 551 genotipten 53'ü seçilerek, dokuz kontrol çeşit kullanılarak moleküler analizler ile birlikte Antalya (ova) ve Konya (yayla) lokasyonlarında ileri testler gerçekleştirildi. Sonuçlara göre; dört SSR markerin verimliliği ovada %38 ve yaylada %39’dur. 40-9187 ve 37-1740 markerleri, iki ortamda da diğer iki marköre göre bitki yüksekliği ile ilişkili QTL'lerin açıklanmasında daha güçlü olarak belirlenmiştir. Bu çalışma, şeker sorgumda biyo-enerji üretimi için değerli genetik kaynakları belirlemek adına iki ortamda da markerler ve bitki boyu arasındaki ilişkinin belirlenmesinde başarılı bir şekilde uygulandığının bildirilmesidir.

Keywords

• Bio-energy
• Biomass
• SSR
• QTL

SSR marker analysis of plant height in sweet sorghum [Sorghum bicolor (L.) Moench]

Abstract

Sorghum (Sorghum bicolor (L.) Moench) is one of the most promising bio-energy crops with the ability to produce high biomass with low input. Plant height that has a significant contribution to gain in bio-ethanol production is among the most important biomass yield components. In the present study, sorghum genotypes were screened with four SSR markers which are associated with plant height QTLs. The molecular assays were confirmed with two different environments in two consecutive years. In the first year of the study, molecular analyses were performed with a sorghum collection consisting of 551 accessions as well as plant height measurements were performed under field condition. In the second year, 53 out of 551 accessions were selected and further tests with nine controls were performed in Antalya (a lowland province) and Konya (a highland province) locations along with molecular marker analyses. The results indicated that four SSR markers efficiency were assessed as 38% at lowland and 39% at highland. Markers 40-9187 and 37-1740 were of more powerful to explain plant height QTLs than the other two markers at two environments. This study reported the successful application of the association between markers and plant height in two environments to identify valuable genetic resources for bio-energy production in sweet sorghum.

Keywords

• Bio-energy
• Biomass
• SSR
• QTL

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