Türkiye’de yetiştirilen beş sığır ırkında MYF5 gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi

Year 2017, Volume: 30 Issue: 1, 35 - 38, 04.04.2017

Cengiz Şahin Bilal Akyüz

Abstract

Bu çalışmada
Türkiye’de yetiştirilen Simental (SİM, n= 75), Holstein (HL, n=100), İsviçre
Esmeri (ES, n=75), Doğu Anadolu Kırmızısı (DAK, n= 40) ve Boz Irk (BI, n=40) sığırlarının MYF5 gen polimorfizminin restiriksiyon parçacık uzunluk
polimorfizmi (RFLP) metoduyla belirlenmesi amaçlanmıştır. MYF5 allelle ve genotipleri elde edilen polimeraz zincir reaksiyonu
(PCR) ürünlerine TaqI kesim enzimi ile muamele edilmesiyle belirlenmiştir. Çalışmada
incelenen ırklar içinde en yüksek AA genotip frekansı DAK (0.20), en yüksek GG
genotip frekansı BI (0.53) ve en yüksek AG genotip frekansı ise ES (0.53)
ırkında görülmüştür. MYF5 geni
yönünden incelenen ırklar arasında en yüksek A allel frekansı DAK ırkında
(0.43), en yüksek G allelinin frekansı ise BI’ta (0.73) bulunmuştur. Çalışma
sonunda incelenen beş sığır ırkının da MYF5
geni yönünden Hardy-Weinberg dengesinde olduğu görülmüştür.

Keywords

Sığır, Genetik belirteç, MYF5, Polimorfizm

Detection of MYF5 gene polymorphism with PCR-RFLP method in five cattle breeds breeding in Turkey

Abstract

The aim of this study was to detection of the MYF5 gene polymorphism in Simmental
(SIM, n=75), Holstein (HL, n=100), Brown Swiss (ES, n=75), East Anatolian Red
(EAR, n=40) and Turkish Grey (TG, n=40) cattle breeds raised in Turkey through
restriction fragment length polymorphism (RFLP) method. The MYF5 alleles and genotypes were detected
by digesting the polymerase chain reaction (PCR) products with TaqI endonuclease enzyme. Among the
breeds examined for MYF5 gene, the
highest AA genotype frequency was found in the EAR cattle breed (0.20); the
highest GG genotype frequency was found in the TG cattle breed (0.53) and the
highest AG genotype frequency was found in the ES cattle breed (0.53). Among
the breeds examined, the highest A allele frequency was found in the EAR cattle
breed (0.43) whereas the highest G allele frequency was found in the TG cattle
breed (0.73). At the end of the study, it was found that examined five cattle
breeds were in Hardy-Weinberg equilibrium (HWE) in terms of MYF5 gene.

Keywords

Cattle, Genetic marker, MYF5, Polymorphism

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