Bazı maddelerin Heterorhabditis bacteriophora HBH ırkının in vitro kitle üretimi üzerine etkileri

Year 2018, Volume: 31 Issue: 3, 209 - 212, 03.12.2018

Tufan Can Ulu Alper Susurluk

https://doi.org/10.29136/mediterranean.437211

Cited By: 1

Abstract

Biyolojik
mücadele, tarımda kimyasal pestisitlere karşı önemli bir alternatif yöntemdir.
Entomopatojen nematodlar (EPN) çoğunlukla toprak kaynaklı zararlı böceklere
karşı kullanılan doğa dostu biyolojik mücadele ajanlarıdır. Uzun yıllardan beri
EPN’ler kitle halinde ticari olarak üretilmektedirler. Kitle üretimi genellik in vitro katı ve sıvı kültürler
içerisinde yapılmaktadır. Kitle üretiminden sonra EPN’ler çeşitli dolgu
maddeleri ile formüle edilmekte ve ticari ürün olarak satılmaktadır. Kitle
üretiminde kullanılan en yaygın maddeler arasında protein ve yağ kaynağı olarak
Nutrient Broth, Yeast ekstraktı, soya unu ve çeşitli bitkisel yağlar
bulunmaktadır. Kitle üretiminin verimini artırmak için bu üretim ortamı
içerisinde farklı maddeler eklenebilmektedir. Bu çalışmada, lesitin ve yumurta
sarısı ve ikisinin birleşiminin Heterorhabditis
bacteriophora HBH ırkının kitle üretiminin verimi üzerindeki etkileri
incelenmiştir. Çalışmada H. bacteriophora
türünün Türkiye’de farklı iklime sahip bölgelere ait izolatlarının
hibritlenmesi ile elde edilmiş HBH ırkı kullanılmıştır. Kitle üretiminin
veriminin hesaplanmasında infektif jüvenil (IJ) sayısı ölçüt olarak
belirlenmiştir. Sonuçlar incelendiğinde tüm uygulamaların kontrole göre
istatistiksel olarak daha etkili olduğu, en iyi uygulamanın ise lesitin
kullanılan ortam ile elde edildiği belirlenmiştir. Bu sonuç ile lesitinin in vitro katı kitle üretiminde ek madde
olarak kullanılabileceği tespit edilmiştir.

Keywords

Entomopatojen nematod, in vitro, Katı ortam, Biyolojik mücadele

Effects of several ingredients on in vitro mass production of Heterorhabditis bacteriophora HBH strain

Abstract

Biological control is a great alternative to chemical pesticides in agriculture. Entomopathogenic nematodes (EPNs) are environmentally safe biocontrol agents that are used mostly against soil-dwelling insect pests. EPNs have been commercially mass produced for several decades. Mass production is generally done in vitro using solid and liquid cultures. After mass production, they are formulated with filler compounds and sold as commercial products. Most common substances for in vitro production are nutrient broth, yeast extract, soyflour and different plant oils for protein and lipid source. To increase the production yield of the mass culture, several compounds can be added to the media. In this study; lecithin, egg yolk and the both together were used in solid medium and effects of these additional compounds were examined on mass production of hybrid Heterorhabditis bacteriophora HBH strain, which was obtained from hybridization of Turkish isolates. Infective juveniles (IJs) of the strain were targeted as mass production yield criteria. According to the results, all treatments showed statistically better yields compared to control, and lecithin was statistically the best treatment among others. Thus, lecithin could be used as an additional compound in in vitro solid mass production media.

Keywords

Entomopathogenic nematode, in vitro, Solid media, Biological control

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