Identification and characterization of lettuce big vein disease (LBVD) in lettuce (Lactuca sativa) crops in Adana and Mersin provinces in Turkey

Year 2019, Volume: 32 Issue: 3, 315 - 321, 01.12.2019

Havva Nur Sağlam Muharrem Arap Kamberoğlu

https://doi.org/10.29136/mediterranean.616046

Cited By: 2

Abstract

This study was carried
out for the purpose of determination of Lettuce big vein disease (LBVD),
identification of the virus or virus complex (MiLBVV and/or LBVaV) causing
disease and characterization of causal agent in the fields where the lettuce
cultivation was made extensively in Adana and Mersin provinces during autumn
and winter months between 2015 and 2017. A total of 160 samples were collected
from lettuce (Lactuca sativa L.) plants
suspected to be infected with LBVD simptomatologically in surveys. In the
result of the ELISA tests, 52 samples were found to be infected with MiLBVV.
RT-PCR studies were performed to identify the causative agents of LBVD by using
specific primer pairs for MiLBVV (MiLBV-F; MiLBVV-R) and LBVaV (VP-248; VP-249)
showed that LBVD is caused by MiLBVV or mixed infection of MiLBVV+LBVaV. LBVaV
infection was not detected alone in lettuce plants with LBVD symptoms. Sequence analyses showed that Adana and Mersin
isolates of MiLBVV formed in separate groups on phylogenetic tree. While
Yakapınar (2-MiLBVV 1 E10), Yumurtalık (6-MiLBVV 1 G10) and Yüreğir (4-MiLBVV 1
F10) isolates from Adana province clustered with Argentina and Iran isolates,
Yenice 1 (11-MiLBVV 2 H10) and Yenice 2 (12-MiLBVV 2 E11) isolates from Mersin
province were in the same group with Netherlands and Egypt isolates in another
group. In addition, the Yüreğir isolate of LBVaV (4-VP 248 A12) clustered with
isolates from Saudi Arabia, United States, United Kingdom, Australia and
Netherlands.

Keywords

Lettuce, LBVD, DAS-ELISA, RT-PCR, Sequence analysis

Supporting Institution

BAP Unit of Cukurova University

Project Number

FYL-2016-7227

Thanks

We would like to thank the Çukurova University Research Fund for their financial support throughout the study

Adana ve Mersin illerinde (Türkiye) yetiştirilen marullarda (Lactuca sativa) marul iri damar hastalığının tanılanması ve karakterizasyonu

Abstract

Bu
çalışma, Marul iri damar hastalığı (Lettuce big-vein disease, LBVD)’nın
saptanması, bu hastalığa neden olan virüs veya virüs kompleksinin (MiLBVV
ve/veya LBVaV) belirlenmesi  ve bu
etmenlerin karakterizasyonu amacıyla, 2015-2017 yılları arasında sonbahar ve
kış aylarında, Adana ve Mersin illerinde yaygın olarak marul yetiştiriciliğinin
yapıldığı alanlarda, yürütülmüştür. Arazi çıkışlarında, simptomatolojik olarak
LBVD ile enfekteli olduğundan şüphelenilen toplam 160 adet marul bitkisinden
örnekleme yapılmıştır. ELISA testleri sonucunda, 52 marul örneği MiLBVV ile
enfekteli bulunmuştur. LBVD’ye sebep olan etmenlerin belirlenmesi amacıyla,
MiLBVV (MiLBVV-F; MiLBVV-R) ve LBVaV (VP-248;VP-249)’ye spesifik primer
çiftleri kullanılarak yapılan RT-PCR çalışmaları sonucunda, MiLBVV veya
MiLBVV+LBVaV karışık enfeksiyonunun varlığı ortaya konmuştur. LBVD simptomu
gösteren marul bitkilerinde tek başına LBVaV enfeksiyonu saptanmamıştır. Sekans
analizlerinde, MiLBVV’nin Adana ve Mersin izolatları filogenetik ağaç üzerinde
kendi aralarında ayrı birer grup oluşturmuşlar ve Adana ilinden, Yakapınar
(2-MilBVV 1 E10), Yumurtalık (6-MiLBVV 1 G10) ve Yüreğir (4-MiLBVV 1 F10)
izolatları, Arjantin ve İran izolatları ile birlikte yer alırken, Mersin
ilinden Yenice 1 (11-MiLBVV 2 H10) ve Yenice 2 (12-MiLBVV 2 E11) izolatları,
Hollanda ve Mısır izolatları ile aynı grupta yer almışlardır. Bunun yanında,
LBVaV’nın Yüreğir izolatı (4-VP 248 A12),
Suudi Arabistan, ABD, Birleşik Krallık, Avustralya ve Hollanda izolatları ile
grup oluşturmuştur.

Keywords

Marul, LBVD, DAS-ELISA, RT-PCR, Sekans analizi

Project Number

FYL-2016-7227

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