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Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Year 2022, Volume: 35 Issue: 2, 83 - 90, 02.08.2022
https://doi.org/10.29136/mediterranean.1083021

Abstract

Pepper mild mottle virus (PMMoV) is a plant virus belonging to the Virgaviridae family; it significantly reduces pepper yield production worldwide. The PMMoV is spread by contaminated seeds and there is no chemical treatment available. Therefore, resistant pepper varieties containing the L4 gene are recommended for the management of PMMoV. A considerable amount of evidence suggests that the L4 gene confers resistance to PMMoV in pepper. The aim of the project is to confirm the status of the L4 gene for resistance to PMMoV in pepper varieties, several inoculations were performed on pepper plants containing L3, L4 resistant genes and susceptible pepper plants without the resistance genes. The L4 resistant plants produced mottling, mosaic, leaf curl, stem necrosis symptoms in the tested pepper plants but there was no amplicon observed with specific primers of PMMoV in RT-PCR analyses. To determine if the L3 and L4 genes are controlling resistance to PMMoV, RT-PCR analyzes were conducted using PMMoV and Tomato brown rugose fruit virus (ToBRFV) where both viruses belong to the same family. The molecular studies revealed that the L4 gene controls resistance mechanisms to PMMoV but it is not able to govern Tobamovirus, ToBRFV. We showed that pepper plants harboring the L3 and L4 gene have the ability to precisely control the mechanism of resistance to PMMoV compared to pepper plants carrying only the L3 gene. A complete genome sequence of PMMoV was obtained and submitted to Genbank with MW523006 accessıon number in the NCBI system.

Supporting Institution

AKDENİZ ÜNİVERSİTESİ

Project Number

FBA-2019-4867

References

  • Afaf A, Eman K, Azza F (2017) Purification and production of antiserum against Pepper mild mottle virus isolated from Saudi Arabia. Research Journal of Biotechnology 12(12): 14-19.
  • Antignus Y, Lachman O, Pearlsman M, Maslenin L, Rosner A (2008) A new pathotype of Pepper mild mottle virus (PMMoV) overcomes the L4 resistance genotype of pepper cultivars. Plant Disease 92: 1033-1037.
  • Buzkan N, Yuzer D (2009) Molecular detection of seed-borne viruses in Kahramanmaras red peppers. Alatarım 8(1): 1-7.
  • Caglar K, Fidan H, Elbeaino T (2013) Detection and Molecular Characterization of Pepper Mild Mottle Virus from Turkey. Journal of Phytopathology 161(6): 434-438.
  • Davino S, Caruso A, Bertacca S, Barone S, Panno S (2020) Tomato brown rugose fruit virus: Seed transmission rate and efficacy of different seed disinfection treatments. Plants 9(11): 1615.
  • Fidan H, Sarı N (2019) Molecular characterization of resistance-breaking Tomato spotted wilt virus (TSWV) isolate medium segment in tomato. Applied Ecology and Environmental Research 17(2): 5321-5339.
  • Fidan H, Sarıkaya P, Yıldız K, Topkaya B, Erkis G, Calis O (2021) Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plant from Turkey. Journal of Integrative Agriculture 20: 2170-2179.
  • Genda Y, Kanda A, Hamada H, Sato K, Ohnishi J, Tsuda S (2007) Two amino acid substitutions in the coat protein of Pepper mild mottle virus are responsible for overcoming the L4 gene-mediated resistance in Capsicum spp. Phytopathology 97: 787-793.
  • Guldur ME, Ozaslan M, Baloglu S, Yilmaz MA (1994) Pepper mild mottle virus in pepper in Turkey. Proceedings of the 9th Congress of the Mediterranean Phytopathological Union, Kusadası, pp: 465-467.
  • Hamada H, Takeuchi A, Kiba S, Tsuda Y, Hikichi T, Okuno T (2002) Amino acid changes in Pepper mild mottle virus coat protein that affect L3 gene-mediated resistance in pepper. Journal of General Plant Pathology 68(2): 155-162.
  • Kabas A, Fidan H, Demirelli M. B (2021) Identification of new sources of resistance to resistance-breaking isolates of Tomato spotted wilt virus. Saudi Journal of Biological Sciences 28(5): 3094-3099.
  • Kabas A, Fidan H, Kucukaydin H, Atan H. N (2022) Screening of wild tomato species and interspecific hybrids for resistance/tolerance to Tomato brown rugose fruit virus (ToBRFV). Chilean Journal of Agricultural Research 82: 1.
  • Kim HJ, Han JH, Yoo JH, Cho HJ, Kim BD (2008) Development of a sequence characteristic amplified region marker linked to the L4 locus conferring broad spectrum resistance to tobamoviruses in pepper plants. Molecules and Cells 25: 205-210.
  • King A.M, Lefkowitz E, Adams M.J, Carstens E.B (2011) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (Vol. 9). Elsevier.
  • Moury B, Verdin E (2012) Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. In Advances in virus research (Vol. 84). Academic Press.
  • Oladokun J.O, Halabi M.H, Barua P, Nath P.D (2019) Tomato brown rugose fruit disease: Current distribution, knowledge and future prospects. Plant Pathology 68: 1579-1586.
  • Petrovic D, Bulajic A, Stankovic I, Ignjatov M, Vujakovic M, Krstic B (2010) Presence and distribution of pepper viruses in Serbia. Ratarstvo i povrtarstvo 47(2): 567-576.
  • Rialch N, Sharma V, Sharma A, Sharma PN (2015) Characterization and complete nucleotide sequencing of Pepper mild mottle virus infecting bell pepper in India. Phytoparasitica 43(3): 327-337.
  • Secrist K, Ali A (2018) First complete genome sequence of Pepper mild mottle virus from chili pepper in the United States. Genome Announcements 6(18): e00331-18.
  • Stecher G, Tamura K, Kumar S (2020) Molecular evolutionary genetics analysis (MEGA) for macOS. Molecular Biology and Evolution 37(4): 1237-1239.
  • Tsuda S, Kubota K, Kanda A, Ohki T, Meshi T (2007) Pathogenicity of Pepper mild mottle virus is controlled by the RNA silencing suppressionactivity of its replication protein but not the viral accumulation. Phytopathology 97: 412-420.

Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Year 2022, Volume: 35 Issue: 2, 83 - 90, 02.08.2022
https://doi.org/10.29136/mediterranean.1083021

Abstract

Pepper mild mottle virus (PMMoV) is a plant virus belonging to the Virgaviridae family; it significantly reduces pepper yield production worldwide. The PMMoV is spread by contaminated seeds and there is no chemical treatment available. Therefore, resistant pepper varieties containing the L4 gene are recommended for the management of PMMoV. A considerable amount of evidence suggests that the L4 gene confers resistance to PMMoV in pepper. The aim of the project is to confirm the status of the L4 gene for resistance to PMMoV in pepper varieties, several inoculations were performed on pepper plants containing L3, L4 resistant genes and susceptible pepper plants without the resistance genes. The L4 resistant plants produced mottling, mosaic, leaf curl, stem necrosis symptoms in the tested pepper plants but there was no amplicon observed with specific primers of PMMoV in RT-PCR analyses. To determine if the L3 and L4 genes are controlling resistance to PMMoV, RT-PCR analyzes were conducted using PMMoV and Tomato brown rugose fruit virus (ToBRFV) where both viruses belong to the same family. The molecular studies revealed that the L4 gene controls resistance mechanisms to PMMoV but it is not able to govern Tobamovirus, ToBRFV. We showed that pepper plants harboring the L3 and L4 gene have the ability to precisely control the mechanism of resistance to PMMoV compared to pepper plants carrying only the L3 gene. A complete genome sequence of PMMoV was obtained and submitted to Genbank with MW523006 accessıon number in the NCBI system.

Project Number

FBA-2019-4867

References

  • Afaf A, Eman K, Azza F (2017) Purification and production of antiserum against Pepper mild mottle virus isolated from Saudi Arabia. Research Journal of Biotechnology 12(12): 14-19.
  • Antignus Y, Lachman O, Pearlsman M, Maslenin L, Rosner A (2008) A new pathotype of Pepper mild mottle virus (PMMoV) overcomes the L4 resistance genotype of pepper cultivars. Plant Disease 92: 1033-1037.
  • Buzkan N, Yuzer D (2009) Molecular detection of seed-borne viruses in Kahramanmaras red peppers. Alatarım 8(1): 1-7.
  • Caglar K, Fidan H, Elbeaino T (2013) Detection and Molecular Characterization of Pepper Mild Mottle Virus from Turkey. Journal of Phytopathology 161(6): 434-438.
  • Davino S, Caruso A, Bertacca S, Barone S, Panno S (2020) Tomato brown rugose fruit virus: Seed transmission rate and efficacy of different seed disinfection treatments. Plants 9(11): 1615.
  • Fidan H, Sarı N (2019) Molecular characterization of resistance-breaking Tomato spotted wilt virus (TSWV) isolate medium segment in tomato. Applied Ecology and Environmental Research 17(2): 5321-5339.
  • Fidan H, Sarıkaya P, Yıldız K, Topkaya B, Erkis G, Calis O (2021) Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plant from Turkey. Journal of Integrative Agriculture 20: 2170-2179.
  • Genda Y, Kanda A, Hamada H, Sato K, Ohnishi J, Tsuda S (2007) Two amino acid substitutions in the coat protein of Pepper mild mottle virus are responsible for overcoming the L4 gene-mediated resistance in Capsicum spp. Phytopathology 97: 787-793.
  • Guldur ME, Ozaslan M, Baloglu S, Yilmaz MA (1994) Pepper mild mottle virus in pepper in Turkey. Proceedings of the 9th Congress of the Mediterranean Phytopathological Union, Kusadası, pp: 465-467.
  • Hamada H, Takeuchi A, Kiba S, Tsuda Y, Hikichi T, Okuno T (2002) Amino acid changes in Pepper mild mottle virus coat protein that affect L3 gene-mediated resistance in pepper. Journal of General Plant Pathology 68(2): 155-162.
  • Kabas A, Fidan H, Demirelli M. B (2021) Identification of new sources of resistance to resistance-breaking isolates of Tomato spotted wilt virus. Saudi Journal of Biological Sciences 28(5): 3094-3099.
  • Kabas A, Fidan H, Kucukaydin H, Atan H. N (2022) Screening of wild tomato species and interspecific hybrids for resistance/tolerance to Tomato brown rugose fruit virus (ToBRFV). Chilean Journal of Agricultural Research 82: 1.
  • Kim HJ, Han JH, Yoo JH, Cho HJ, Kim BD (2008) Development of a sequence characteristic amplified region marker linked to the L4 locus conferring broad spectrum resistance to tobamoviruses in pepper plants. Molecules and Cells 25: 205-210.
  • King A.M, Lefkowitz E, Adams M.J, Carstens E.B (2011) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (Vol. 9). Elsevier.
  • Moury B, Verdin E (2012) Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. In Advances in virus research (Vol. 84). Academic Press.
  • Oladokun J.O, Halabi M.H, Barua P, Nath P.D (2019) Tomato brown rugose fruit disease: Current distribution, knowledge and future prospects. Plant Pathology 68: 1579-1586.
  • Petrovic D, Bulajic A, Stankovic I, Ignjatov M, Vujakovic M, Krstic B (2010) Presence and distribution of pepper viruses in Serbia. Ratarstvo i povrtarstvo 47(2): 567-576.
  • Rialch N, Sharma V, Sharma A, Sharma PN (2015) Characterization and complete nucleotide sequencing of Pepper mild mottle virus infecting bell pepper in India. Phytoparasitica 43(3): 327-337.
  • Secrist K, Ali A (2018) First complete genome sequence of Pepper mild mottle virus from chili pepper in the United States. Genome Announcements 6(18): e00331-18.
  • Stecher G, Tamura K, Kumar S (2020) Molecular evolutionary genetics analysis (MEGA) for macOS. Molecular Biology and Evolution 37(4): 1237-1239.
  • Tsuda S, Kubota K, Kanda A, Ohki T, Meshi T (2007) Pathogenicity of Pepper mild mottle virus is controlled by the RNA silencing suppressionactivity of its replication protein but not the viral accumulation. Phytopathology 97: 412-420.
There are 21 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Makaleler
Authors

Hakan Fidan 0000-0002-0384-9486

Ailar Gonbadi 0000-0003-4621-4082

Pelin Sarıkaya 0000-0002-6133-6128

Özer Çalış 0000-0002-7219-1219

Project Number FBA-2019-4867
Publication Date August 2, 2022
Submission Date March 4, 2022
Published in Issue Year 2022 Volume: 35 Issue: 2

Cite

APA Fidan, H., Gonbadi, A., Sarıkaya, P., Çalış, Ö. (2022). Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene. Mediterranean Agricultural Sciences, 35(2), 83-90. https://doi.org/10.29136/mediterranean.1083021
AMA Fidan H, Gonbadi A, Sarıkaya P, Çalış Ö. Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene. Mediterranean Agricultural Sciences. August 2022;35(2):83-90. doi:10.29136/mediterranean.1083021
Chicago Fidan, Hakan, Ailar Gonbadi, Pelin Sarıkaya, and Özer Çalış. “Investigation of Activity of Tobamovirus in Pepper Plants Containing L4 Resistance Gene”. Mediterranean Agricultural Sciences 35, no. 2 (August 2022): 83-90. https://doi.org/10.29136/mediterranean.1083021.
EndNote Fidan H, Gonbadi A, Sarıkaya P, Çalış Ö (August 1, 2022) Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene. Mediterranean Agricultural Sciences 35 2 83–90.
IEEE H. Fidan, A. Gonbadi, P. Sarıkaya, and Ö. Çalış, “Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene”, Mediterranean Agricultural Sciences, vol. 35, no. 2, pp. 83–90, 2022, doi: 10.29136/mediterranean.1083021.
ISNAD Fidan, Hakan et al. “Investigation of Activity of Tobamovirus in Pepper Plants Containing L4 Resistance Gene”. Mediterranean Agricultural Sciences 35/2 (August 2022), 83-90. https://doi.org/10.29136/mediterranean.1083021.
JAMA Fidan H, Gonbadi A, Sarıkaya P, Çalış Ö. Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene. Mediterranean Agricultural Sciences. 2022;35:83–90.
MLA Fidan, Hakan et al. “Investigation of Activity of Tobamovirus in Pepper Plants Containing L4 Resistance Gene”. Mediterranean Agricultural Sciences, vol. 35, no. 2, 2022, pp. 83-90, doi:10.29136/mediterranean.1083021.
Vancouver Fidan H, Gonbadi A, Sarıkaya P, Çalış Ö. Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene. Mediterranean Agricultural Sciences. 2022;35(2):83-90.

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