Potential of entomopathogenic fungi as biological control agents of Yponomeuta malinellus Zeller, 1838 (Lepidoptera: Yponomeutidae)

Year 2022, Volume: 35 Issue: 3, 121 - 128, 02.12.2022

Hilal Tosun Derya Baki Fedai Erler

https://doi.org/10.29136/mediterranean.1082196

Abstract

The apple ermine moth, Yponomeuta malinellus Zeller, 1838 (Lepidoptera Yponomeutidae), is a common pest of apple trees in Asia and Europe, and it has spread to North America. In apple growing regions of Turkey, the population of this pest may increase from time to time, requiring a separate control measure. In such cases, Turkish apple growers generally rely on synthetic insecticides to control this pest. The present study aimed to evaluate indigenous isolates of some entomopathogenic fungi (EPFs) against the pest as potential biological control agents. In the pathogenicity tests, 14 EPF isolates that belong to 4 fungal species [Beauveria bassiana (Bals.) Vuill. – 7, Clonostachys rosea (Link) Schroers – 3, Isaria farinosa (Holmsk.) Fr. – 2 and Purpureocillium lilacinum (formerly known as Paecilomyces lilacinus (Thom) Samson) (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson – 2] were assayed against the 4th instar larvae of Y. malinellus under laboratory conditions. All the EPF isolates were tested against the pest with three different conidial suspensions (1 × 107, 1 × 108 and 1 × 109 conidia ml-1), using the spray method. The results of pathogenicity assays demonstrated that the effectiveness of the isolates increased with increasing concentration and elapsed time up to 9 days after treatment. Of the 14 isolates tested, 3 B. bassiana isolates (BbDm-1, BbDs-2 and BbKm-3) were the most pathogenic, causing mortalities between 96.7% and 100% at the highest concentration 9 days post treatment. All the results suggest that the most pathogenic above-mentioned 3 isolates of B. bassiana have a significant biocontrol potential against Y. malinellus.

Keywords

Apple ermine moth, Entomopathogenic fungi, Molecular identification, Pathogenicity, Yponomeuta malinellus

References

• Alramadan Y, Mamay M (2019a) The importance of entomopathogenic fungi in the control of agricultural pests and promising fungal entomopathogens in the field application. 1st International Gobeklitepe Agriculture Congress. Şanlıurfa, Turkey, pp. 266-274.
• Alramadan Y, Mamay M (2019b) The importance of entomopathogenic bacteria in the control of agricultural pests and promising these entomopathogens in the field. 1st International Gobeklitepe Agriculture Congress. Şanlıurfa, Turkey, pp.258-265.
• Alramadan Y, Mamay M (2019c) What is the role of entomopathogenic viruses in the control of agricultural pests and their future in the field application? 1st International Gobeklitepe Agriculture Congress. Şanlıurfa, Turkey, pp. 301-309.
• Alramadan Y, Mamay M (2019d) The importance of entomopathogenic nematode and their role in the control of agricultural pests. 1st International Gobeklitepe Agriculture Congress. Şanlıurfa, Turkey, pp. 301-309.
• Anonymous (2012) Yponomeuta malinellus (Apple Ermine Moth) - Fact Sheet. Canadian Food Inspection Agency pp. 13.
• Baki D, Tosun HS, Erler F (2021) Indigenous entomopathogenic fungi as potential biological control agents of rose sawfly, Arge rosae L. (Hymenoptera: Argidae). Turkish Journal of Zoology 45(7): 517-525.
• Butt TM, Jackson C, Magan N (2001) Introduction fungal biological control agents: progress, problems and potential. In: Fungi as Biocontrol Agents: Progress, Problems and Potential. Butt, T. M., C. Jackson and N. Magan, (Eds), CAB International, Wallingford, pp. 1-8.
• Butt TM (2002) Use of entomogenous fungi for the control of insect pests. In: Mycota. Esser, K. and J.W. Bennett, (Eds), Springer, Berlin, pp. 111-134.
• Castro-Vásquez RM, Molina-Bravo R, Hernández-Villalobos S, Vargas-Martínez A, González-Herrera A, Montero-Astúa M (2021) Identification and phylogenetic analysis of a collection of Beauveria spp. Isolates from Central America and Puerto Rico. Journal of Invertebrate Pathology 184:107642. doi: 10.1016/j.jip.2021.107642.
• Doyle J, Doyle J (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
• Erturk O (2016) Insecticidal effects of some Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) isolates on the larvae of Yponomeuta malinellus Zell. (Lepidoptera: Yponomeutidae) and Cydia pomonella L. (Lepidoptera: Tortricidae). Harran Journal of Agricultural and Food Science 20(3): 183-191.
• Fancelli M, Dias AB, Delalibera IJ, Cerqueira de Jesus S, Souza do Nascimento A, Oliveira e Silva S (2013) Beauveria bassiana strains for biological control of Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae) in Plantain. BioMed Research International. doi.org/10.1155/2013/184756.
• Gencer L (2003) The Parasitoids of Yponomeuta malinellus Zeller (Lepidoptera: Yponomeutidae) in Sivas. Turkish Journal of Zoology 27: 43-46.
• Glare TR, Inwood A (1998) Morphological and genetic characterization of Beauveria spp. from New Zealand. Mycological Research 102: 250-256.
• Glare TR (2004) Molecular characterization in the entomopathogenic fungal genus Beauveria. Laimburg Journal 1: 286-298.
• Glare TR, Reay SD, Nelson TL, Moore R (2008) Beauveria caledonica is a naturally occurring pathogen of forest beetles. Mycological Research 112: 352-360.
• Goettel MS, Inglis GD (1997) Fungi Hyphomycetes: Manual of Techniques in Insect Pathology. Academic Press, San Diego, pp. 213-248.
• Goettel MS, Eilenberg J, Glare TR (2010) Entomopathogenic fungi and their role in regulation of insect populations. In: Insect Control: Biological and Synthetic Agents. Gilbert, L.I. and S. Gill, (Eds), Academic Press, London, pp. 387-432.
• Iren Z (1960) Researches on ermine moth (Yponomeuta) species, their host plants, brief biology and controls in Ankara Region. Ziraat Vekaleti C-4, pp. 141 (in Turkish).
• Kimber I (2021) UK Moths. https://ukmoths.org.uk/species/yponomeuta-malinellus. Accessed 2 November, 2021.
• Kuhlmann U, Carl KP, Mills NJ (1988) Quantifying the impact of insect predators and parasitoids on populations of apple ermine moth, Yponomeuta malinellus (Lepidoptera: Yponomeutidae), in Europe. Bulletin of Entomological Research 88: 165-175.
• Mamay M, Mutlu C (2019) Optimizing container size and rearing density for rapid and economic mass rearing of Oenopia conglobata (Linnaeus, 1758) (Coleoptera: Coccinellidae). Turkish Journal of Entomology 43(4): 395-408.
• McDonough LM, Davis HG, Smithhisler CL, Voerman S, Chapman PS (1990) Apple ermine moth, Yponomeuta malinellus Zeller two components of female sex pheromone gland highly effective in field trapping tests. Journal of Chemical Ecology 16(2): 477-486.
• Narmanlioglu HK, Coruh S (2018) Parasitoids of the apple ermine moth, Yponomeuta malinellus Zeller, 1838 (Lepidoptera: Yponomeutidae), in the Çoruh Valley, Erzurum Province, Turkey. Turkish Journal of Entomology 41: 357-365.
• Rehner S (2001) Primers for elongation factor 1–α (EF1–α). Available at: http://ocid.nacse.org/research/deephyphae/EF1primer.
• Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84-98.
• Serna-Domínguez MG, Gilda Y (2019) Andrade-Michel, Rogelio Rosas-Valdez, Patricia Castro-Félix, Hugo C. Arredondo-Bernal, Adrien Gallou, High genetic diversity of the entomopathogenic fungus Beauveria bassiana in Colima, Mexico, Journal of Invertebrate Pathology 163: 67-74.
• Sönmez C, Mamay M (2018) Biological control in sustainable agriculture. In Proceedings of the International GAP Agriculture & Livestock Congress, Sanliurfa, Turkey, 25-27 April 2018.
• Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731-2739.
• Unruh TR, Congdon BD, LaGassa E (1993) Yponomeuta malinellus Zeller (Lepidoptera: Yponomeutidae), a new immigrant pest of apples in the Northwest: phenology and distribution expansion, with notes on efficacy of natural enemies. Pan-Pacific Entomologist 69: 57-70.
• Vidal C, Fargues J (2007) Climatic constraints for fungal bioinsecticides, In S. Ekesi, and N. K. Maniania (eds.), Use of entomopathogenic fungi in biological pest management. Research Signpost Inc., Kerala, pp. 39-55.
• White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods and Applications. Academic Press, New York, USA, pp. 315-322.
• Wraight SP, Jackson MA, De Kock SL (2001) Production, stabilization, and formulation of fungal biocontrol agents. In: Fungi as Biocontrol Agents: Progress, Problems and Potential. Butt, T.M., C.W. Jackson and N. Magan, (Eds), CAB International, Wallingford, pp. 253-258.