Evaluation of iron, iron binding capacity, transferrin, some oxidative stress markers and hematological parameters in foot and mouth disease in cattle

Year 2024, Volume: 9 Issue: 1, 224 - 229, 12.06.2024

Mert Sezer Tahir Gezer Oğuz Merhan Enes Akyüz Kadir Bozukluhan Gürbüz Gökce

Abstract

This study aimed to investigate the changes in serum iron, iron binding capacity, transferrin, some oxidative stress markers, and hematological and biochemical parameters in cattle infected with foot-and-mouth disease and to reveal their importance. The animal material of the study was composed of 20 Simmental cattle between 6 and 12 months of age, which were diagnosed with foot-and-mouth disease based on the results of the clinical and laboratory examinations (patient group), and the control group was composed of 10 animals selected from a different herd, with the same age group and breed characteristics and fed with the same ration. Among the hematological parameters examined in the study, the total leukocyte count (WBC (x103/μL)) was found to be higher in the patient group with statistical significance compared to the control group (P<0.05). Among the biochemical parameters, iron (Fe (μg/dL)), total iron binding capacity (TIBC (μg/dL)), phosphorus (P (mg/dl)), magnesium (Mg (mg/dl)), and glucose (mg/dl) ) levels were found to be significantly lower (P<0.001); the transferrin saturation (TS (%)), reduced glutathione (GSH (mg/dL)), and alkaline phosphatase (ALP (U/L)) were found to be significantly lower (P<0.05); and Malondialdehyde (MDA (μmol/L)), creatine kinase (CK ((U/L)), and creatinine (Crea (mg/dl)) were found to be significantly higher (P<0.05) in the patient group compared to the control group. Consequently among the biochemical parameters examined in the study, the changes in the Fe (μg/dL), TIBC (μg/dL), TS (%), GSH (mg/dL), and MDA (μmol/L) levels were observed.

Keywords

cattle, foot and mouth disease, oxidative stress, transferrin

Ethical Statement

Kafkas University Local Ethics Committee of Animal Experiments (KAU-HADYEK/2022-032)

References

• Akyuz, E., & Gokce, G. (2021). Neopterin, procalcitonin, clinical biochemistry, and hematology in calves with neonatal sepsis. Tropical Animal Health Production, 53, 354. https://doi.org/10.1007/s11250-021-02779-z
• Akyuz, E., Makav, M., Kuru, M., & Bayram, P. (2021). The effect of Tarantula cubensis extract on gentamicin-induced acute kidney injury in ovariectomized rats. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27, 559-566. https://doi.org/10.9775/kvfd.2021.25390
• Akyuz, E., & Aydin, U. (2022). Prognostic value of haptoglobin and ceruloplasmin levels determined in cows with adhesive and non-adhesive traumatic reticuloperitonitis. Kafkas Universitesi Veteriner Fakultesi Dergisi, 28, 337-344. https://doi.org/10.9775/kvfd.2021.27040
• Akyuz, E., Merhan, O., Aydın, U., Sezer, M., Kuru, M., Karakurt, E., Yıldız, U., Bozukluhan, K., Batı, Y.U., Yıldız, A., & Gökce, G. (2022a). Neopterin, procalcitonin, total sialic acid, paraoxonase-1 and selected haematological indices in calves with aspiration pneumonia. Acta Veterinaria Brno, 91, 115–124. https://doi.org/10.2754/avb202291020115
• Akyuz, E., Sezer, M., Kuru, M., & Naseri, A. (2022b). Changes in hematology, some clinical biochemical parameters and mineral levels in neonatal calves with sepsis due to diarrhea. Van Veterinary Journal, 33(1), 26-30. https://doi.org/10.36483/vanvetj.1066050
• Asif, N., Ijaz, A., Rafi, T., Haroon, Z.H., Bashir, S., & Ayyub, M. (2016). Diagnostic accuracy of serum iron and total iron binding capacity (tibc) in iron deficiency. State Journal of the College of Physicians and Surgeons Pakistan, 26(12), 958-961.
• Baydar, E., & Dabak, M. (2014). Serum iron as an indicator of acute inflammation in cattle. International Journal of Dairy Science, 97, 222-228. https://doi.org/10.3168/jds.2013-6939
• Beutler, E., Duron, O., & Kelly, B.M. (1963). Improved method for the determination of blood glutathione. Journal of Clinical Medicine, 61, 882-888.
• Bozukluhan, K., Merhan, O., Kiziltepe, S., Ergin Egritag, H., Akyuz, E., & Gokce H.I. (2021). Determination of haptoglobin, some biochemical and oxidative stress parameters in calves with pneumonia. Fresenius Environmental Bulletin, 30, 9485-9489.
• Coskun, A., & Sen, I. (2012). Haematological, biochemical and coagulation changes in calves with endotoxemia. Journal of Agricultural Science and Technology, 7, 37-41.
• Cenesiz, S. (2020). The role of oxidant and antioxidant parameters in the infectious diseases: a systematic literature review. Kafkas Universitesi Veteriner Fakultesi Dergisi ,26, 849-858.
• Elsayed, M.E., Sharif, M.U., & Stack, A.G. (2016). Transferrin saturation: a body iron biomarker. Advances in Clinical Chemistry, 75, 71-97. https://doi.org/10.1016/bs.acc.2016.03.002
• Gakuya, D.W., Mulei, C.M., & Wekesa, S.B. (2011). Use of ethnoveterınary remedıes ın the management of foot and mouth disease lesions in a diary herd. Trends and Challenges of Traditional Medicine in Africa, 8, 165-169. https://doi.org/10.4314/ajtcam.v8i2.63204
• Ganz, T., & Nemeth, E. (2009). Iron sequestration and anemia of inflammation. Seminars in Hematology, 46, 387-393. https://doi.org/10.1053/j.seminhematol.2009.06.001
• Gomme, P.T., McCann, K.B., & Bertolini, J. (2005). Transferrin: structure, function and potential therapeutic actions. Drug Discovery Today Technologies, 10, 267-273. https://doi.org/10.1016/S1359-6446(04)03333-1
• Gozzelino, R., & Arosio, P. (2016). Iron homeostasis in health and disease. International Journal of Molecular Sciences, 17, 130. https://doi.org/10.3390/ijms17010130
• Gokce, G., Gokce, H.İ., Güneş, V., Erdoğan, H.M., & Çitil, M. (2004). Alterations in some haematological and biochemical parameters in cattle suffering from foot and mouth disease. Turkish Journal of Veterinary and Animal Sciences, 28, 723-727.
• Kar, J., Ahad, A., Nath, S.K., Islam, Z., & Sarker, M.S. (2015). Haemato-biochemical aspects of Foot and Mouth disease in cattle in Chittagong, Bangladesh. İnternational Journal of Molecular Biology, 3, 62-65. http://dx.doi.org/10.14737/journal.jimb/2015/3.3.62.65
• Khoshvaghti, A., Askari, A., Nazifi, S., & Ghane, M. (2014). Evaluation of some antioxidant enzymes in cattle ınfected with foot and mouth virüs. Journal of Faculty of Veterinary Medicine İstanbul University, 40, 70-75.
• Krupaa, R.J., Hariharan, R., Babu, N.A., & Masthan, K.M.K. (2020). Alkaline phosphatase and its clinical importance a review. European Journal of Molecular & Clinical Medicine, 7, 1409-1413.
• Lepper, C., Partridge, T.A., & Fan, C.M. (2011). An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. Development, 138, 3639-3646. https://doi.org/10.1242/dev.067595
• Marreiro, D.N., Cruz, K.J.C., Morais, J.B.S., Beserra, J.B., Severo, J.S. & Oliveira, A.R.S. (2017). Zinc and oxidative stress: Current mechanisms. Antioxidants, 6, 24. https://doi.org/10.3390/antiox6020024
• Mates, J.M. (2000). Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153, 83-104. https://doi.org/10.1016/s0300-483x(00)00306-1
• Matsufuji, H., Ochi, H., & Shibamoto, T. (2006). Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food and Chemical Toxicology, 44, 236-241. https://doi.org/ 10.1016/j.fct.2005.07.004
• Merhan, O., & Özcan, A. (2010). Peripartum dönemdeki koyunlarda seruloplazmin, haptoglobin, fibrinojen, albümin ve transferrin düzeylerinin araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 7, 13-20.
• Ozcan, O., Erdal, H., Cakirca, G., & Yonden, Z. (2015). Oxidative stress and its impacts on intracellular lipids, proteins and DNA. Journal of Clinical and Experimental Investigations, 6, 331-336.
• Salim, S.A.S., Talb, A.O.Q., Saad, A.M.S., & Yousif, A.S. (2019). Clinico-pathological and biochemical aspects of foot and mouth disease in calves. Advances in Animal and Veterinary Sciences, 7, 835-843. http://dx.doi.org/10.17582/journal.aavs/2019/7.10.835.843
• Sezer, M., & Gokce, G. (2021). Studies on sTREM-1, Mr-Pro-Adrenomedullin (Mr-Pro-Adm), clinical biochemistry and hematology in calves suspected of sepsis. Kafkas Üniversitesi Sağlık Bilimleri Enstitüsü, PhD Thesis, Kars.
• Sezer, K., & Keskin, M. (2014). Role of the free oxygen radicals on the pathogenesis of the diseases. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 28, 149-156.
• Sen, I., Altunok, V., Ok, M., Coskun, A., & Constable, P.D. (2009). Efficacy of oral rehydration therapy solutions containing sodium bicarbonate or sodium acetate for 109 treatment of calves with naturally acquired diarrhea, moderate dehydration, and strong ion acidosis. Journal of the American Veterinary Medical Association, 234, 926-934. https://doi.org/10.2460/javma.234.7.926
• Talukder, S., Gabai, G., & Celi, P. (2015). The use of digital infrared thermography and measurement of oxidative stress biomarkers as tools to diagnose foot lesions in sheep. Small Ruminant Research, 127, 80-85.
• Tapiero, H., Gate, L., & Tew, K.D. (2001). Iron: deficiencies and requirements. Biomedicine and Pharmacotherapy, 55, 324-332. https://doi.org/10.1016/s0753-3322(01)00067-1
• Tsukano, K., Shimamori, T., & Suzuki, K. (2020). Serum iron concentration in cattle with endotoxaemia. Acta veterinaria Hungarica, 68, 53-58. https://doi.org/10.1556/004.2020.00016
• Uzlu, E., Karapehlivan, M., Erdoğan, H.M., Kızıltepe, Ş., Erkılıç, E.E., Deveci, H.A., Gökçe, E., Kaya, İ., & Çitil, M. (2016). Serum and saliva sialic acid and oxidative stress parameters changes in bulls with foot and mouth disease. Kafkas Üniversitesi Veteteriner Fakültesi Dergisi, 22, 321-325.
• Wong, C.L., Yong, C.Y., Ong, H.K., Ho, K.L., & Tan, W.S. (2020). Advances in the diagnosis of foot and mouth disease. Frontiers in Veterinary Science. 7. https://doi.org/10.3389/fvets.2020.00477
• Yilmaz, O., & Gokce, G. (2017). Investigations on clinic, haematology, biochemistry, oxidative stress, acute phase proteins in infectious respiratory disease complex (BRDC) in cattle. Atatürk Üniversitesi Veteriner Bilimler Dergisi, 12, 34-44
• Yoshioka, T., Kawada, K., Shimada, T., & Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism againist active-oxygen toxicity in the blood. American Journal of Obstetrics & Gynecology, 135, 372-376. https://doi.org/10.1016/0002-9378(79)90708-7
• Yoruk, I.H., Tanritanir, P., Dede, S., Ceylan, E., & Ragbetli, C. (2014). Antioxidant vitamins and microminerals in cows with foot and mouth dısease. Indian Journal of Animal Research, 48, 593-596.
• Yurdakul, G., & Sarıtas, Z.K. (2013). Evaluation of clinic, radiographic and some biochemical blood serum and synovial fluid parameters of arthritis cases in calves. Kocatepe Veterinary Journal, 6, 13-22.