THE EFFECT OF DIFFERENT HORMONE CONCENTRATIONS ON CALLUS FORMATION IN COTTON ANTHERS

Year 2017, Volume: 3 Issue: 2, 93 - 97, 29.12.2017

MEDET Korkunç , Adem Bardak Remzi Ekinci

https://doi.org/10.23884/mejs.2017.3.2.03

Abstract

Cotton
is a hot climatic industrial plant commonly planted on both the tropical and
subtropical regions of the world. Four different genotypes of cotton, Aşkabat-100 (G.
barbadense ), Coker-312 and Stoneville-468 (G. hirsutum ), were studied for
callus induction. The cotton anthers extracted from immature flower drafts
(cotton gins) were used as explants. Cotton anthers taken from different length
immature cotton combs  were used as
explants. After samples taken from cotton anthers of different lengths (2, 3,
4, 5 mm) were subjected to sterilization with different NaOCl concentrations (%
10, % 20 ve % 30) prepared in sterilized glass containers for surface
sterilization, the immature anthers found in the obtained were extracted and
placed in feeding media with various amounts of different hormones to induce
callus formation.  After the seeding is
done, lids of the petri dishes have been closed and to prevent the air inflow
and outflow were covered with  parafilms,
then the petri dishes were left for dark in the climate room for about 30-60
days. The experiments were performed with repetitions of 3. Seeding was done
every three days and the callus size and the regeneration rates that resulted
from 5 week dark environment incubation were determinedOnce the anthers were
transferred to the induction media, one-hour cold (4°C) shock and one-hour hot
shock (40°C) were applied to them, they were kept in dark for a while and were
left for collaganase in climate room at 16/24 light regime. All experiments in
this study were performed in triplicates. 
As the result of the experiments, the highest rate of callus formation was
observed in Cooker 312 supplemented with 2mg/mL of NAA and 2 mg/L of BA
hormones. Callus formation was also higher in the condition where NAA was used
than the media supplemented with 2,4-D. Additionally, callus formation showed
better results in cold and hot shock applied anthers compared to the ones that
were not shocked. 

Keywords

Cotton, Comb, anther

References

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