standart besiyerine alternatif uygun maliyetli besiyeri Spirulinanın pigment üretimini ve antibakteriyel etkisini artıkmaktadır.

Abstract

Spirulina (Arthrospira platensis), sahip olduğu biyoaktif bileşikler nedeniyle yüksek katma değere sahip bir mikro algdir. Spirulina süper gıda ve geleceğin gıdası olarak kabul edilmektedir. Gıda, ilaç ve boya endüstrileri gibi çeşitli sektörlerde birçok uygulama alanına sahiptir. Spirulina için standart büyüme ortamının daha uygun maliyetli bir alternatifle değiştirilmesi, daha sürdürülebilir bir mikroalg üretimi sağlayacaktır. Bu nedenle bu çalışmada, farklı büyüme ortamları [standart Zarrouk ortamı (ZM), Low Medium (LM) ve Modified Low medium (MLM)] 5L biyoreaktörlerde test edilmiş ve Spirulina'daki biyoaktif bileşikler (klorofil a, klorofil b, toplam klorofil, karotenoid ve fikosiyanin) ölçülmüştür. Biyokütlenin element analizi ICP-OES ile gerçekleştirilmiş ve Spirulina ekstraktlarının antibakteriyel aktivitesi analiz edilmiştir. Sonuçlar, Spirulina'daki biyoaktif bileşik içeriklerinin MLM'de yetiştirildiğinde arttığını ve bunun da ekstraktların antibakteriyel aktivitesini artırdığını göstermiştir. MLM'de şelatör bulunmaması ortamın maliyetini önemli ölçüde azaltmıştır. Ayrıca, daha yüksek pigment üretimi, pigment üretimi amacıyla yüksek maliyetli mikro besin çözeltisi kullanılmasına gerek olmadığını göstermiş ve bu da ortam maliyetini ciddi anlamda düşürmüştür. Sonuç olarak, MLM'de ZM ve LM'ye kıyasla daha fazla biyoaktif bileşik üretimi mikroalglerin ekonomik değerini artırmaktadır. Hem azalan maliyetler hem de artan biyoaktif bileşik içerikleri MLM'yi Spirulina üretimi için daha iyi bir seçim haline getirmektedir.

Keywords

• Arthrospira platensis
• growth medium
• phycocyanin
• carotenoid
• chlorophyll
• antibacterial activity

A cost-effective alternative to standard medium enhances the pigment production and antibacterial activity of Spirulina

Abstract

Spirulina (Arthrospira platensis) is a microalga with a high value due to the bioactive compounds it possesses. Spirulina is considered to be the super food and food of the future. It has many applications in various industries such as food, pharmaceuticals and dye industries. Replacing the standard growth medium for Spirulina with a more cost-effective alternative would offer a more sustainable production of microalgae. That is why in this study, there different growth media [standard Zarrouk’s medium (ZM), Low Medium (LM) and Modified Low medium (MLM)] were tested in 5L bioreactors and bioactive compounds (chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and phycocyanin) in Spirulina were measured. Elemental analysis of the biomass was carried out by ICP-OES, and antibacterial activity of Spirulina extracts were analyzed. The results showed that the bioactive compound contents in Spirulina were elevated upon cultivation in MLM, which increased the antibacterial activity of the extracts. Lack of chelator in MLM significantly decreased the cost of the medium. Moreover, higher production of pigments suggested that there was no need to use high-cost micronutrient solution for the aim of pigment production, which definitely lowered the cost of medium. As a result, enhanced bioactive compound production on MLM compared to ZM and LM increases the economic value of the microalgae. Both reduced costs and increased bioactive compound contents make MLM a better choice for Spirulina production

Keywords

• Arthrospira platensis
• growth medium
• phycocyanin
• carotenoid
• chlorophyll
• antibacterial activity

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