Defne (Laurus nobilis L.) Uçucu Yağının Balık Patojeni Pseudomonas Türlerine Karşı Antibakteriyel ve Antibiyofilm Aktivitelerinin Belirlenmesi

Abstract

Yüksek biyoaktif bileşen konsantrasyonuna sahip olması nedeniyle Defne (Laurus nobilis) esansiyel yağının, çeşitli terapötik özelliklere sahip olduğu bilinmektedir. Bu çalışma ile Laurus nobilis yapraklarından ekstrakte edilen esansiyel yağın kimyasal bileşiminin yanı sıra antipsödomonal ve antibiyofilm etkileri de dahil olmak üzere biyolojik özelliklerinin analiz edilmesi amaçlanmıştır. Minimum İnhibasyon Konsantrasyonu (MİK) değerlerinin, P. fluorescens için 31,25 µg/mL, P. putida için 31,25 µg/mL ve P. aeruginosa için 62,5 µg/mL olduğu tespit edilmiştir. P. fluorescens ve P. putida biyofilmlerinin, MİK altı dozlarda Laurus nobilis esansiyel yağının anti-biyofilm aktivitesi tarafından baskılandığı görülmüştür. Sonuçlar, minimum inhibasyon konsantrasyonlarında esansiyel yağın, Pseudomonas türü biyofilm oluşumunu etkili bir şekilde önlediğini ortaya koymuştur. Sonuç olarak, Laurus nobilis esansiyel yağlarının, antibiyofilm potansiyeline sahip muhtemel antibakteriyel ajan kaynakları olduğu sonucuna varılmıştır.

Keywords

• antibakteriyel aktivite
• antibiyofilm aktivite
• uçucu yağ
• defne

Determination of Antibacterial and Antibiofilm Activities for Laurel (Laurus nobilis L.) Essential Oil Against the Fish Pathogen Pseudomonas Species

Abstract

The essential oil derived from Laurus nobilis exhibits a high concentration of bioactive components, imparting various therapeutic characteristics. This study aimed to analyze the chemical composition of the essential oil extracted from Laurus nobilis leaves as well as its biological properties, including its antipseudomonal and antibiofilm actions. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that 1,8-cineole (%48.43) and α-terpinyl acetate (14.78) were the major compounds present in the essential oil (EO). While, the minimum inhibitory concentration (MIC) values of Laurus nobilis essential oils (LEO) against P. fluorescens and P. putida were determined as 31.25 µg/mL, it was 62.5 µg/mL for P. aeruginosa. LEO, at a MIC level of 31,25µg/mL, exhibited significant inhibition of Pseudomonas species biofilm formation except for P. aeruginosa. Based on its demonstrated antibacterial and antibiofilm potential, LEO holds promise as a prospective source of antibacterial agents.

Keywords

• antibacterial activity
• antibiofilm activity
• essential oil
• laurel
• pseudomonas

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