Farklı Su Sıcaklıklarında Gül Ağacı (Aniba rosaeodora) Esansiyel Yağının ve 2-fenoksietanolün Japon Balığı (Carassius auratus) Üzerindeki Anestezik Etkileri

Year 2023, Volume: 9 Issue: 2, 15 - 26, 28.12.2023

Barış Şahin , Volkan Kızak

https://doi.org/10.58626/menba.1372338

Abstract

Bu çalışmada, gül ağacı esansiyel yağının ve 2-fenoksietanolün farklı su sıcaklıklarında Japon balıkları üzerinde anestezik etkilerinin incelenmesi amaçlanmıştır. 14°C ve 20°C su sıcaklıklarında anestezik denemeler gerçekleştirilmiştir. Gül ağacı esansiyel yağı (ArEO) için 250, 300 ve 350 µl/lt, 2-fenoksietanol (2-PE) için ise 600, 700 ve 800 µl/lt konsantrasyonlar denenmiştir. 14°C’de ideal ArEO konsantrasyonu 300 µl/lt, 20°C’de ise 250 µl/lt olarak belirlenmiştir. 2-PE için her iki su sıcaklığında da ideal konsantrasyon 800 µl/lt olarak tespit edilmiştir. ArEO ve 2-PE’ün yüksek konsantrasyonları, daha kısa indüksiyon sürelerine ve daha uzun iyileşme sürelerine neden olmuştur. Anestezik ajanların konsantrasyonları indüksiyon süreleri ile negatif, iyileşme süreleri ile pozitif korelasyon göstermiştir. ArEO için 14ºC’de AD ve RF süreleri arasında zayıf negatif bir ilişki, 20ºC’de ise negatif bir ilişki bulunmuştur. 14ºC ve 20ºC’lerde 2-PE anesteziğinde AD ve RF süreleri arasında negatif ilişki tespit edilmiştir. ArEO AD süreleri ve ArEO konsantrasyonları arasında 14ºC ve 20ºC’lerde kuvvetli negatif bir ilişki vardır ve benzer bir ilişki 2-PE için de bulunmuştur. Bununla birlikte, farklı su sıcaklıklarında her iki anestezik ajanda RF süreleri ve konsantrasyonlar arasında kuvvetli pozitif ilişki tespit edilmiştir. Artan su sıcaklığının bir sonucu olarak her iki anestezik maddede anestezi ve iyileşme sürelerinin kısaldığı görülmüştür. ArEO ve 2-PE için indüksiyon ve iyileşme sürelerinde su sıcaklığı ve anestezik konsantrasyon interaksiyonu önemli bulunmuştur.

Keywords

Anestezi, su sıcaklığı, Japon balığı, Aniba rosaeodora

Ethical Statement

T.C. Munzur Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’nun E-63614754-050.01.04-2300006839 sayılı kararı (Toplantı sayısı 2022-21 / Karar no. 21-01) ile etik kurallar çerçevesinde bu çalışma gerçekleştirilmiştir.

Thanks

Bu araştırma makalesi, Barış Şahin’in yüksek lisans tezinden üretilmiştir.

References

• Aydın, İ., Akbulut, B., Küçük, E. & Kumlu, M. (2015). Effects of temperature, fish size and dosage of clove oil on anaesthesia in turbot (Psetta maxima Linnaeus, 1758). Turkish Journal of Fisheries and Aquatic Sciences, 15:899-904. https://doi.org/10.4194/1303-2712-v15_4_13
• Aydın, A., Akhan, S., Gümüş, E. & Özbaş, M. (2019). Anesthetic efficacy of clove oil and 2-fhenoxyethanol on Doctor fish, Garra rufa (Heckel, 1843). Boletim Do Instituto De Pesca, 45(4):e506. https://doi.org/10.20950/1678-2305.2019.45.4.506
• Can, E., Kızak, V., Özçiçek, E. & Can, S. S. (2017). The efficacy of chamomile (Matricaria chamomilla) oil as a promising anesthetic agent for two freshwater aquarium fish species. The Israeli Journal of Aquaculture-Bamidgeh, 1437(69):1-8.
• Can, E., Kızak, V., Özçiçek, E. & Can S. S. (2018). Anesthetic potential of geranium (Pelargonium graveolens) oil for two cichlid species, Sciaenochromis fryeri and Labidochromis caeruleus. Aquaculture, 491:59-64. https://doi.org/10.1016/j.aquaculture.2018.03.013
• Can, E., Kızak, V., Özçiçek, E. & Can, S. S. (2019). Anesthetic Efficiency of Three Medicinal Plant Oils for Aquatic Species: Coriander Coriandrum sativum, Linaloe Tree Bursera delpechiana, and Lavender Lavandula hybrida. Journal of Aquatic Animal Health, 31:266-273. https://doi.org/10.1002/aah.10081
• Clarke, A. & Johnston, N. M. (1999). Scaling of metabolic rate with body mass and temperature in teleost fish. Journal of Americal Ecology, 68:893-905.
• Correia, A. M., Pedrazzani, A. S., Mendonca, R. C., Massucatto, A., Ozorio, R. A. & Tsuzuki, M. Y. (2018). Basil, tea tree and clove essential oils as analgesics and anaesthetics in Amphiprion clarkii (Bennett, 1830). Brazilian Journal of Biology, 78(3):436-442. https://doi.org/10.1590/1519-6984.166695
• Cunha, M. A., Barros, F. M. C., Oliveira Garcia, L., Lima Veeck, A. P., Heinzmann, B. M., Loro, V. L., & Baldisserotto, B. (2010). Essential oil of Lippia alba: A new anesthetic for silver catfish, Rhamdia quelen. Aquaculture, 306:403-406. https://doi.org/10.1016/j.aquaculture.2010.06.014
• Fernandez-Parra, R., Donnelly, T. M., Pignon, C., Noirault, A. & Zilberstein, L. (2017). Immersion anesthesia with alfaxalone in a goldfish (Carassius auratus). Journal of Exotic Pet Medicine, 26:276-282. https://doi.org/10.1053/j.jepm.2017.08.003
• Hamackova, J., Lepicova, A., Kozak, P., Stupka, Z., Kouril, J. & Lepic, P. (2004). The efficacy of various anaesthetics in tench (Tinca tinca L.) related to water temperature. Veterinarni Medicina, 49:467-472.
• Heldwein, C. G., Silva, L. L., Gai, E. Z., Roman, C., Parodi, T. V., Bürger, M. E., Baldisserotto, B., Flores, E. M. & Heinzmann, B. M. (2014). S-(+)-Linalool from Lippia alba: sedative and anesthetic for silver catfish (Rhamdia quelen). Veterinary Anaesthesia and Analgesia, 41:621-629. https://doi.org/10.1111/vaa.12146
• Hoseini, S. M., Rajabiesterabadi, H. & Tarkhani, R. (2015). Anaesthetic efficacy of eugenol on iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) in different size classes. Aquaculture Research, 46:405-412. https://doi.org/10.1111/are.12188
• Hoskonen, P. & Pirhonen, J. (2004). Temperature effects on anaesthesia with clove oil in six temperate-zone fishes. Journal of Fish Biology, 64:1136-1142.
• Kızak, V., Can, E., Danabaş, D. & Seyhaneyıldız Can, S. (2018a). Evaluation of anesthetic potential of rosewood (Aniba rosaeodora) oil as a new anesthetic agent for goldfish (Carassius auratus). Aquaculture, 493:296-301. https://doi.org/10.1016/j.aquaculture.2018.05.013
• Kizak, V., Can, E., Can, Ş. S. & Özçiçek, E. (2018b). Anesthetic efficacy of Cymbopogon citratus essential oil as a herbal agent in two ornamental fish species. The Israeli Journal of Aquaculture-Bamidgeh, 70:1-8.
• Kızak, V., Can, E. & Seyhaneyıldız Can, S. (2020). Potential anesthetic properties of Bay Leaf (Laurus nobilis) essential oil compared with 2-phenoxyethanol on Blue Dolphin Cichlid, Cyrtocara moorii. The Israeli Journal of Aquaculture-Bamidgeh, 72:1-9. http://doi.org/10.46989/IJA.72.2020.961461
• Kucuk, S. (2010). Efficacy of tricaine on Peocilia latipinna at different temperatures and concentrations. African Journal of Biotechnology, 9(5):755-759. https://doi.org/10.5897/AJB09.1353
• Limma-Netto, J. D., Sena, A. C. & Copatti, C. E. (2016). Essential oils of Ocimum basilicum and Cymbopogon flexuosus in the sedation, anesthesia and recovery of tambacu (Piaractus mesopotamicus male x Colossoma macropomum Female). Boletim do Instituto de Pesca, 42(3):727-733. https://doi.org/10.20950/1678-2305.2016v42n3p727
• Mazandarani, M., Hoseini, S. M. & Ghomshani, M. D. (2017). Effects of linalool on physiological responses of Cyprinus carpio (Linnaeus, 1758) and water physico-chemical parameters during transportation. Aquaculture Research, 48:5775-5781. https://doi.org/10.1111/are.13400
• Mazandarani, M. & Hoseini, S. M. (2017). Menthol and 1,8-cineole as new anaesthetics in common carp, Cyprinus carpio (Linnaeus, 1758). Aquaculture Research, 48:3041-3051. https://doi.org/10.1111/are.13136
• Mirghaed, A. T., Ghelichpour, M. & Hoseini, S. M. (2016). Myrcene and linalool as new anesthetic and sedative agents in common carp, Cyprinus carpio- comparison with eugenol. Aquaculture, 464:165-170. https://doi.org/10.1016/j.aquaculture.2016.06.028
• Mirghaed, A. T., Yasari, M., Mirzargar, S. S. & Hoseini, S. M. (2018). Rainbow trout (Oncorhynchus mykiss) anesthesia with myrcene: efficacy and physiological responses in comparison with eugenol. Fish Physiology and Biochemistry, 44:919-926. https://doi.org/10.1007/s10695-018-0481-5
• Mitjana, O., Bonastre, C., Tejedor, M. T., Garza, L., Esteban, J. & Falceto, M. V. (2018). Simultaneous effect of sex and dose on efficacy of clove oil, tricaine methanesulfonate, 2-phenoxyethanol and propofol as anaesthetics in guppies, Poecilia reticulata (Peters). Aquaculture Research, 49:2140-2146. https://doi.org/10.1111/are.13668
• Mylonas, C. C., Cardinaletti, G., Sigelaki, I. & Polzonetti-Magni, A. (2005). Comperative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus auratus) at different temperature. Aquaculture, 246:467-481. https://doi.org/10.1016/j.aquaculture.2005.02.046
• Park, I. S., Lee, T. H. & Lim, S. G. (2018). Anesthetic efficacy and physiological responses of clove oil on juvenile and adult red spotted grouper, Epinephelus akarra. Fisheries and Aquatic Sciences, 21:1-6. https://doi.org/10.1186/s41240-018-0100-5
• Rezende, F. P., Pascoal, L. M., Vianna, R. A. & Lanna, E. A. T. (2017). Sedation of Nile tilapia with essential oils: Tea tree, clove, eucalyptus, and mint oils. Revista Caatinga, 30(2):479-486. https://doi.org/10.1590/1983-21252017v30n223rc
• Ross, L. G. & Ross, B. (2008). Anaesthetic and sedative techniques for aquatic animals, 3rd edition. Wiley-Blackwell Publishing, Oxford, İngiltere, 240s.
• Santos, S., Ghanawi, J. & Saoud, I. P. (2015). Effects of water temperature and body weight on anaesthetic efficiency in marbled rabbitfish (Siganus rivulatus). Aquaculture Research, 46:928-936. https://doi.org/10.1111/are.12249
• Sarrazin, S. L. F., Oliveira, R. B., Maia, J. G. S. & Mourao, R. H. V. (2016). Antibacterial activity of the rosewood (Aniba rosaeodora and A. parviflora) linalool-rich oils from the Amazon. European Journal of Medicinal Plants, 12:1-9. https://doi.org/10.9734/EJMP/2016/22901
• Schoettger, R. A. & Julin, A. M. (1967). Efficacy of MS-222 as an anesthetic on four salmonids. Investigations in Fish Control 13, Report-Federal Government Series, U.S. Fish and Wildlife Service, A.B.D, 15s.
• Schurmann, H. & Steffensen, J. F. (1997). Effects of temperature, hypoxia and activity on the metabolism of juvenile Atlantic cod. Journal of Fish Biology, 50:1166-80.
• Sena, A. C., Teixeira, R. R., Ferreira, E. L., Heinzmann, B. M., Baldisserotto, B., Caron, B. O., Schmidt, D., Couto, R. D. & Copatti, C.E. (2016). Essential oil from Lippia alba has anaesthetic activity and is effective in reducing handling and transport stress in tambacu (Piaractus mesopotamicus X Colossoma macropomum). Aquaculture, 465:374-379. https://doi.org/10.1016/j.aquaculture.2016.09.033
• Silva, L. L., Balconi, L. S., Gressler, L. T., Garlet, Q. I., Sutili, F. J., Vargas, A. P. C., Baldisserotto, B., Morel, A. F. & Heinzmann, B. M. (2017). S-(+)- and R-(−)-linalool: a comparison of the in vitro anti-Aeromonas hydrophila activity and anesthetic properties in fish. Anais da Academia Brasileira de Ciências, 89:203-212. https://doi.org/10.1590/0001-3765201720150643
• Silva, L. A., Martins, M. A., Santo, F. E., Oliveira, F. C., Chaves, F. C. M., Chagas, E. C., Martins, M. L. & Campos, C. M. (2020). Essential oils of Ocimum gratissimum and Zingiber officinale as anesthetics for the South American catfish Pseudoplatystoma reticulatum. Aquaculture, 528:735595. https://doi.org/10.1016/j.aquaculture.2020.735595
• Skår, M. W., Haugland, G. T., Powell, M. D., Wergeland, H. I. & Samuelsen, O. B. (2017). Development of anaesthetic protocols for lumpfish (Cyclopterus lumpus L.): Effect of anaesthetic concentrations, sea water temperature and body weight. PLoS ONE, 12(7):e0179344. https://doi.org/10.1371/journal.pone.0179344
• Sneddon, L. U. (2012). Clinical anesthesia and analgesia in fish. Journal of Exotic Pet Medicine, 21:32-43. https://doi.org/10.1053/j.jepm.2011.11.009
• Van der Salm, A. L., Pavlidis, M., Flik G. & Wendelaar Bonga, S. E. (2006). The acute stress response of red porgy, Pagrus pagrus, kept on a red or white background. General and Comparative Endocrinology, 145:247-253. https://doi.org/10.1016/j.ygcen.2005.09.010
• Velisek, J., Wlasow, T., Gomulka, P., Svobodova, Z. & Novotny, L. (2007). Effects of 2-phenoxyethanol anaesthesia on sheatfish (Silurus glanis L.). Veterinární medicína, 52:103-110.
• Weber, R. A., Peleteiro, J. B., García Martín, L. O. & Aldegunde, M. (2009). The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup, 1858). Aquaculture, 288:147-150. https://doi.org/10.1016/j.aquaculture.2008.11.024
• Weyl, O., Kaiser, H. & Hecht, T. (1996). On the efficacy and mode of action of 2- phenoxyethanol as an anaesthetic for goldfish (Carassius auratus), at different temperatures and concentrations. Aquaculture Research, 27:757-764.
• Yigit, N. O. & Kocaayan, H. (2023). Efficiency of thyme (Origanum onites) and coriander (Coriandrum sativum) essential oils on anesthesia and histopathology of rainbow trout (Oncorhynchus mykiss). Aquaculture, 562:738813. https://doi.org/10.1016/j.aquaculture.2022.738813
• Zahl, I. H., Kissling, A., Samuelsen, O. B. & Hansen, M.K. (2009). Anaesthesia of Atlantic cod (Gadus morhua) - Effect of pre-anaesthetic sedation, and importance body weight, temperature and stress. Aquaculture, 295:52-59. https://doi.org/10.1016/j.aquaculture.2009.06.019