Nikotin ve Antagonisti Mecamylaminin Kurbağa (Rana ridibunda) İskelet Kası Üzerine Etkileri

Year 2008, Volume: 1 Issue: 3 , 14 - 20 , 01.09.2008

Dilek Kumargal Ülkü Çömelekoğlu Ali Aşkın

https://izlik.org/JA79HS32EP

Abstract

AbstractThe Effects of Nicotine and Its Antagonist Mecamylamine on Skeletal Muscle of Frog (Rana ridibunda) Objective: In this study, we investigated the effects of nicotine which is the active ingredient of smoke and a therapeutic agent mecamylamine which is the nicotinic antagonist on contraction force of isolated frog gastrocnemius muscle depending on application time. Method: The study was composed of three experimental groups; 10-4M nicotine, 10-4M mecamylamine and 10-4M nicotine + 10-4M mecamylamin combination. Ten frog gastrocnemius muscles were investigated in each group (n=30). Measurements were taken at 0th, 30th, 60th, 90th, 120th minutes. Measurements at 0th minute were the control of the preparation of muscles. Contraction recordings were obtained by BIOPAC MP 100 Acqusition System and maximum contraction force was used. Result: Nicotine and its antagonist mecamylamine decreased the contraction force 48% and 81% at 120th minute respectively when compared with control (p<0.05). Nicotine and mecamylamine combination group displayed no changes in contraction force at each threatment time segment. Conclusion: As a result nicotine showed an inhibition effect on contraction force of skeletal muscle in threatment time. This effect of nicotine was reversed by mecamylamine. On the other hand mecamylamine inhibited the contraction force of skeletal muscle alone.

Keywords

nicotine , mecamylamine , gastrocnemius muscle , frog.

Nikotin ve Antagonisti Mecamylaminin Kurbağa (Rana ridibunda) İskelet Kası Üzerine Etkileri

https://izlik.org/JA79HS32EP

Abstract

Türkçe Özet:Amaç: Bu çalışmada, sigaranın etken maddesi olarak kabul edilen nikotin ile birçok alanda tedavi edici ajan olarak da kullanılan nikotin antagonisti mecamylaminin, izole kurbağa gastrokinemius kası kasılma kuvveti üzerine etkisinin, uygulama süresine bağlı olarak değişiminin araştırılması amaçlanmıştır. Yöntem: Çalışmada 10-4M nikotin, 10-4M mecamylamin ve 10-4M nikotin + 10-4M mecamylamin kombinasyon grubu olmak üzere üç ayrı deney grubu oluşturulmuştur. Her bir deney grubu için on gastrokinemius kası çalışılmıştır (n=30). Süreye bağlı etkileri inceleyebilmek için ölçümler 0., 30., 60., 90. ve 120. dakikalarda alınmıştır. Sıfırıncı dakikadaki ölçüm kas preparatın kendi kontrolüne karşılık gelmektedir. Kasılma eğrilerini kaydetmek için BIOPAC MP 100 Acqusition sistemi kullanılmıştır. Elde edilen kayıtlardan maksimum kasılma kuvveti ölçülmüştür. Bulgular: Nikotin ve mecamylaminin ayrı ayrı uygulandığı deney gruplarında her iki madde kasılma kuvvetini kontrole göre uygulamanın 120. dakikasında sırasıyla %48 ve %81 oranında azaltmıştır (p<0.05). Nikotin ve mecamylamin kombinasyonunun uygulandığı deney grubunda ise aynı dozlar kasılma kuvvetinde kontrole göre herhangi bir değişikliğe yol açmamıştır (p>0.05). Sonuç: Bu çalışma sonucunda nikotin zamana bağlı olarak iskelet kası kasılma kuvveti üzerine inhibe edici bir etki göstermiştir. Nikotinin bu etkisi mecamylamin tarafından ortadan kaldırılmıştır. Ancak bir nikotin antagonisti olan mecamylamin tek başına iskelet kası kasılma kuvvetini inhibe edici bir etki sergilemektedir.

Keywords

nikotin , mecamylamin , gastrokinemius kası , kurbağa.

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