AbstractRole of Sarcoplasmic-Endoplasmic Reticulum Ca2+ ATPase Inhibition on Contractions of Central Ear Artery of Rabbit Objective: This study investigated whether the store-operated (capacitative) Ca2+ entry is coupled to contraction in a peripheral blood vessel, central ear artery of the rabbit. Method: Rabbits (male, 2.5-3 kg, n=5) were sacrificed via intracardiac sodium pentobarbital injection. Endothelium-denuded central ear artery rings (3 mm) were isolated and placed in organ baths containing physiological salt solution (Krebs, 37°C) gassed with (95% O2+5% CO2). Following the stabilization period, tissues were stimulated with 60 mM potassium chloride. A selective sarcoplasmic reticulum-Ca2+ ATPase inhibitor, cyclopiazonic acid was applied during the steady-state contraction of each agents. Results: Cyclopiazonic acid at concentration (10 mM) which shown to deplete intracellular Ca2+ stores did not elicit any contraction. Potassium chloride- (30 mM) and norepinephrine- (100 nM) induced contractions were not enhanced by cyclopiazonic acid whereas serotonin contractions were significantly potentiated in the presence of cyclopiazonic acid (p<0.01). Conclusion: The data suggest that increased intracellular Ca2+ concentration resulting from store-operated Ca2+ entry may also be restricted in non-contractile intracellular compartments in peripheral artery smooth muscle cells and coupled to contraction via serotonin receptor activation. In conclusion, unlike adrenoceptors, the data suggest that serotonin receptors could also be located in the plasma membrane over these subsarcolemmal compartments similar to that suggested for endothelin and prostaglandin receptors.
cyclopiazonic acid store-operated Ca2+ entry sarcoplasmic-endoplasmic reticulum Ca2+ ATPase inhibition rabbit central ear artery serotonin
Amaç: Bu çalışmada depo-kontrollü (kapasitatif) Ca+2 girişinin periferal bir damar olan tavşan merkezi kulak arterinde kasılmaya kenetlenip kenetlenmediğinin araştırılması amaçlandı. Yöntem: Tavşanlar (erkek, 2.5-3 kg, n=5) intrakardiyak sodyum pentobarbital enjeksiyonu ile öldürüldüler. Endotel hücreleri uzaklaştırılmış merkezi kulak arter halkaları (3 mm) izole edildi ve gazlandırılan (%95 O2+%5 CO2) fizyolojik tuz çözeltisi (Krebs, 37oC) içeren organ banyolarına alındı. Stabilizasyon sonrası dokular 60 mM potasyum klorür ile uyarıldı. Her bir ajana bağlı kasılabilir yanıtların plato fazında selektif sarkoplazmik-endoplazmik retikulum-Ca+2 ATPaz inhibitörü siklopiazonik asit uygulandı. Bulgular: Hücre içi Ca+2 depolarını tamamen boşalttığı gösterilen derişimde (10 mM) siklopiazonik asit herhangi bir kasılmaya neden olmadı. Potasyum klorür (30 mM) ve noradrenalinin (100 nM) neden olduğu kasılmalar siklopiazonik asit ile artmazken siklopiazonik asit varlığında serotonin (300 nM) kasılmaları belirgin düzeyde potansiyelize oldu (p < 0.01). Sonuç: Bulgular, depo-kontrollü Ca+2 girişi sonucu artan hücre içi Ca+2 derişiminin periferik damarlarda da kasılabilir aparat içermeyen sitozolik kompartmanlarda “hapsedilebileceğini” ve serotonin reseptör etkinleşmesi ile kasılmaya kenetlenebileceğini düşündürmektedir. Sonuç olarak bulgularımız, noradrenalin reseptöründen farklı olarak, serotonin reseptörlerinin de endotelin ve prostaglandin reseptörleri için önerilene benzer şekilde sıkı olarak sınırlandırılmış özelleşmiş kompartmanların plazma membranları üzerinde yerleşmiş olabileceğini düşündürmektedir.
siklopiazonik asit depo-kontrollü Ca+2 girişi sarkoplazmik-endoplazmik retikulum Ca+2 ATPaz inhibisyonu tavşan kulak arteri serotonin
Primary Language | Turkish |
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Journal Section | Articles |
Authors | |
Publication Date | September 1, 2008 |
Submission Date | June 13, 2014 |
Published in Issue | Year 2008 Volume: 1 Issue: 3 |
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