AbstractThe Effect of Radiation Damage to Malonyldialdehyde Levels in Testes and Kidney Tissue and to Prevent with Melatonin Objective: The humans are frequently to be expose with radiation for therapy. Radiation is to applied for spesific localisation but whoole body is attacted with radiation. In this study we investigated the effect of radiotherapy to urogenital system and whether melatonin prevents the possible effect of radioterapy or not. Method: We formed four groups of Wistar rat and each group involves seven rats. The rats weigh 200-250 gram. In radiation process, SSD: 90 cm, 90% isodose curve was used in Picker C 9 Cobalt 60 machine. At each seance, 32 cGy/minute and 11 hour 30 minute, totally 360 cGy, in 40x40 cm space gamma rays were given. The seances were done at 12 hours intervals. Without giving radiation to the first group, at 12 hours intervals, serum physiological was applied to intraperitoneum (RK). To the second group, after 2x360 cGy radiation was applied; serum physiological was applied to intraperitoneum at 12 hours intervals (RS). To the third group, after 2x360 cGy radiation was applied, at 12 hours interval, melatonin was applied in peritoneum 2x5 mg/kg/per days for five days (RM). To the fourth group was neither radiation, nor melatonin was applied (SH). On the fifth day, by decapitating all the groups, testes and kidney tissues was to be extirpation. The tissues was to be homogenization and looking for tissue malonyldialdehyde levels. Malonyldialdehyde is a parameter which to expose the effect of free radicals. Results: There was not much difference between the SH group which took neither radiation nor serum physiologic to the RK group which took no radiation but intreperitoneal serum physiologic (p>0.05). We found remarkable difference between the RK group which took no radiation but intreperitoneal serum physiologic and the RS group which took both radiation and intraperitoneal serum physiologic (p<0.05). There was difference between the RS group which took both radiation and intraperitoneal serum physiologic and RM group which took both radiation and melatonin (p<0.05). Conclusion: Melatonin, to be decrease of radiation damage for testes and kidney tissue.
Türkçe Özet:Amaç: İnsanlar tedavi amaçlı olarak sıklıkla radyasyonla karşılaşmaktadır. Radyasyon belirli bölgelere uygulanır, fakat tüm vücut radyasyondan etkilenmektedir. Bu çalışmada radyoterapinin ürogenital sistem üzerine etkileri ve bu etkilerin melatonin ile önlenip önlenemeyeceğinin araştırılması amaçlandı. Yöntem: 200-250 gr arasında değişen Wistar tipi sıçanlardan her biri yedi sıçan içeren dört grup oluşturuldu. Radyasyon uygulamasında Picker C 9 Cobalt 60 cihazında, SSD: 90 cm, %90 izodoz eğrisi kullanıldı. Her seansta 32 Gy/dk\'lık 11dk 30sn süreyle toplam 360 cGy olmak üzere 40x40 cm\'lik alanda gamma ışını verildi. Seanslar 12 saat arayla uygulandı. Birinci gruba radyasyon uygulamaksızın 12 saat arayla periton içine serum fizyolojik uygulandı (RK). İkinci gruba 2x360 cGy radyasyon uyguladıktan sonra 12 saat arayla periton içine serum fizyolojik uygulandı (RS). Üçüncü gruba 2x360 cGy radyasyon uygulandıktan sonra 12 saat arayla periton içine 2x5 mg/kg/gün beş gün süreyle melatonin uygulandı (RM). Dördüncü gruba radyasyon ya da intraperitoneal hiçbir ilaç uygulaması yapılmadı (SH). Beşinci günün sonunda bütün gruplar dekapite edilerek testisler ve böbrekler alındı. Dokular homojenize edilerek lipit peroksidasyonunun sekonder bir ürünü olarak oluşan malonyldialdehit (MDA) düzeylerine bakıldı. Bulgular: Radyasyon ya da intraperitoneal hiçbir ilaç uygulamasının yapılmadığı SH grubuyla radyasyon almaksızın intraperitoneal serum fizyolojik uygulanan RK grubu arasında MDA düzeyleri bakımından anlamlı bir fark bulunamadı p>0.05). Radyasyon almaksızın intraperitoneal serum fizyolojik alan RK grubu ile radyasyon+intraperitoneal serum fizyolojik alan RS grubu arasında MDA düzeyleri bakımından istatistiksel olarak anlamlı fark bulundu (p<0.05). RS grubu ile radyasyon+melatonin alan RM grubu arasında MDA düzeyleri bakımından anlamlı fark bulundu (p<0.05). Radyasyon hasarının melatonin ile azaltılabileceği gözlendi. Sonuç: Melatonin, böbrek ve testislerde oluşan radyasyon hasarını azaltmakta yararlı olabilir.
Primary Language | Turkish |
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Journal Section | Articles |
Authors | |
Publication Date | March 1, 2009 |
Submission Date | June 13, 2014 |
Published in Issue | Year 2009 Volume: 2 Issue: 1 |
MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.
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