Vitamin D reseptör geni fokI polimorfizminin temporomandibular eklem dejenerasyonu ile ilişkisi

Yıl 2019, Cilt: 12 Sayı: 1, 72 - 79, 28.04.2019

Ayça Dilara Yılmaz

https://doi.org/10.26559/mersinsbd.423103

Cited By: 3

Öz

Amaç: Temporomandibular Eklem
Düzensizliği (TME-İD) ve eklem osteoartritine (TME-OA) yatkınlık genetik
varyasyonlarla ilişkili olabilir. Vitamin D Reseptörü (VDR) gen polimorfizmleri buna adaydırlar.
Bu çalışmanın
amacı, VDR FokI polimorfizminin
temporomandibular
eklem dejenerasyonu ile ilişkili olup olmadığını
cinsiyete göre değerlendirmekti. Yöntem: 58 osteoartritik
TME-İD hastası (32.07.±8.1) ve kontrol olarak 71 TME-İD olmayan birey
(28.28±5.95)
çalışmaya dahil edildi. Kan örneklerinden
DNA izolasyonu standart proteinaz
K/fenol-kloroform
metodu ile yapıldı. VDR geni FokI polimorfizmi, polimeraz zincir reaksiyonunu (PZR) takiben restriksiyon
fragment uzunluk
polimorfizmi
(RFLP) yöntemi ile
araştırıldı. Bulgular:
FokI genotip
dağılımları
(rs2228570,  C>T) grupları arasında istatistiksel olarak
farklı idi (p=0.026, χ²=7.2). Heterozigot Ff genotipi, FF genotipine göre istatistiksel olarak farklı idi (OR=0.43, %95 GA:0.2-0.92, p=0.028). TME-İD’li kadınlarda heterozigot Ff
genotipi FF genotipine göre farklılık sınırında idi (OR=0.43, %95 CI:0.16-1.10, p=0.07). Yine TME-İD’li kadınlarda ff genotipi
ile FF genotipi arasında fark olmamasına rağmen (%95 GA: 0.29-26.03, p=0.37) risk faktörü 2.77 kat fazla idi. TME-İD/kontrol grubu ve kadın kontrollerde de F ve f alellerinin dağılımları farklı değildi. Sonuç: FokI polimorfizminin
TME-İD/TMEOA gelişmesi üzerinde önemli bir etkisi olduğu düşünülebilir. ff
genotipi, TME-İD hastalarında ve TME-İD’li kadınlarda temporomandibular eklem
dejenerasyonu ile ilgili olabilir.

Anahtar Kelimeler

temporomandibular eklem osteoartriti, Temporomandibular eklem internal düzensizliği, D Vitamini reseptör geni, FokI polimorfizm

The relation between Vitamin D receptor gene fokI polymorphism and degeneration of the temporomandibular joint

Öz

Objective: Predisposition to temporomandibular joint
internal
derangement (TMJ-ID) and osteoarthritis
of the joint
(TMJOA)
might be related to genetic variations.
Vitamin D receptor (VDR) gene
polymorphisms
are the candidates for
association with the disorder. The aim of
this study was to evaluate
whether VDR
FokI polymorphism is associated
with the degeneration of the temporomandibular joint
by
gender. 

Methods: The study included 58 unrelated TMJ-ID patients
(32.07
±8.1) and 71 healthy controls (28.28 ±5.95) without TMJ-ID. DNA extraction
was achieved by standard
proteinase
K/phenol-chloroform
method
from the blood samples.
 VDR gene FokI polymorphism was investigated
by a polymerase
chain reaction
(PCR) based restriction
fragment length polymorphism
(RFLP) assay.
Results: The genotype distributions
of FokI (rs2228570, C>T) showed statistically
significant results (p=0.026,
χ²=7.2). Ff heterozygote genotype was statistically different as compared to FF genotype (OR=0.43, 95% CI: 0.2-0.92,
p=0.028).
Heterozygote
Ff genotype
was different
comparing to the FF
genotype in TMJ-ID women (OR=0.43, 95% CI: 0.16-1.10,
p=0.07).
Although the difference between ff and FF genotype

were not different, ff genotype was a 2.77 fold
risk factor
(95% CI:0.29-26.03,  p=0.37).
The distributions of F and f alleles were not different between the groups of
overall TMJ-ID vs. controls and women TMJ-ID vs. controls. Conclusion: The results of this study suggested that FokI polymorphism might
present susceptibility to TMJ-ID/TMJOA. ff genotype might
be associated
with the degeneration of temporomandibular joint in TMJ-ID patients and TMJ-ID women.

Anahtar Kelimeler

Temporomandibular joint internal derangement, temporomandibular joint osteoarthritis, vitamin D receptor gene, FokI polymorphism

Kaynakça

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